The correlation of laser correlation spectroscopy and cytokines in the mixed saliva of patients undergoing orthodontic treatment

The aim was to correlate the content of cytokines/chemokines in the oral fluid of patients undergoing orthodontic treatment with the results of laser correlation (LC) spectroscopy in order to develop approaches to prenosological diagnostics. Methods. Oropharyngeal flushes and oral fluid obtained from patients were used. Subfractional composition of oropharyngeal flushes was analyzed by LC spectroscopy. Cytokines/chemokines content in the oral fluid was determined with MILLIPLEX® MAP kit. Results. Based on the results of LC spectroscopy, all patients were divided into 3 groups: those with normological spectra, with allergic- like and intoxication-like metabolic shifts. Cytokine content analysis showed a decrease in IgM, IgA, IgG1, IgG2, IgG3, IgG4 content, both in groups with allergic-like and intoxication-like shifts. A positive correlation was found between contribution to light scattering of particles larger than 545.06 nm and the concentration of IL-12P70 and MIP-1β in the group with allergic-like shifts. Intoxication-like shifts were characterized by an increase in the contribution of small particles (1.9-11.34 and 20-90 nm), which was correlated with total amount of small cytokines (15-70 kDa). According to discriminant analysis, the maximum difference between the patients with allergic-like shifts and other groups was associated with IP-10 content. Differences in the contribution to light scattering of small particles were related with IL- 12P40 and FGF-2. Conclusion. The combined use of LC spectroscopy and cytokine profile assessment to determine correlations will allow identifying groups of specific biologically active molecules associated with observed metabolic shifts and will aid in the development of prenosological diagnostics and pathogenetic corrective methods

laser correlation spectroscopy, cytokines, chemokines, saliva