Alterations in mRNA expression of rat astrocytes CO-cultured with C6 glioma cells and impact of glioma-conditioned astrocytes on dendritic cell maturation
- Authors: Chernysheva A.A.1, Chekhonin I.V.1, Sosnovtseva A.O.1, Cherepanov S.A.1,2, Kardashova K.S.1, Gurina O.I.1, Silantyev A.S.3, Pavlova S.A.4, Pavlova G.V.4,5,6, Savelieva T.A.7,8, Loshhenov V.B.7,8, Chekhonin V.P.1,2
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Affiliations:
- V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Pirogov Russian National Research Medical University
- National Scientific Research Center on Addictions - a branch of the V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
- Institute of Institute of Higher Nervous Activity and Neurophysiology of the Russian Federation
- Sechenov First Moscow State Medical University
- «N.N. Burdenko National Medical Research Center of Neurosurgery» of the Ministry of Health of the Russian Federation
- Prokhorov General Physics Institute of the Russian Academy of Sciences
- National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
- Issue: Vol 19, No 5 (2021)
- Pages: 16-28
- Section: Articles
- URL: https://journals.eco-vector.com/1728-2918/article/view/113476
- DOI: https://doi.org/10.29296/24999490-2021-05-03
- ID: 113476
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Open Access
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Abstract
Introduction. Under the influence of glioma cells, peritumoral zone astrocytes may acquire tumorigenic phenotype, particularly due to the suppression of immunocompetent cells. The aim of the study. Investigate the changes in the gene expression profile of glioma-conditioned rat astrocytes and the impact of conditioned astrocyte cellular extracts (lysates) on dendritic cell maturation. Methods. We compared glioma-conditioned astrocytes with C6 glioma cells or native astrocytes. Astrocyte conditioning was achieved by indirect co-culturing of native astrocytes with C6 glioma cells. The expression profile was assessed with real-time PCR. The phenotype of dendritic cells pulsed with extracts of glioma-conditioned astrocytes, C6 glioma cells and native astrocytes was studied with flow cytometry and ELISA. Dendritic cells were also used for rat immunization. We measured serum interferon-γ dynamics and cytokine production (interferon-γ, interleukins 4 and 10) by mononuclear cells of immunized rats. Results. Conditioned astrocytes showed a tendency to upregulation of interleukin 6 mRNA. They also demonstrated lower expression of PDGFB chain mRNA. Glioma cells expressed higher levels of nestin, GFAP, and CD44 mRNA. CCL2 chemokine mRNA was expressed at lower amounts in glioma cells and glioma-conditioned astrocytes. Dendritic cells pulsed with extracts of conditioned astrocytes yielded a lower increase of serum and mononuclear-derived interferon-γ after rat immunization. These dendritic cells exposed less CD11b/c but produced more interleukin 12 in comparison. Conclusion. The findings suggest that astrocytes may undergo phenotype transformation under the influence of glioma cells and obtain traits different from glioma cells or native astrocytes and similar to both cellular types at the same time
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About the authors
Anastasia Alekseevna Chernysheva
V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: aachernysheva512@gmail.com
junior researcher
Ivan Vladimirovich Chekhonin
V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: chekhonin@yandex.ru
research scientist
Anastasia Olegovna Sosnovtseva
V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: sollomia@yandex.ru
junior researcher
Sergey Aleksandrovich Cherepanov
V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Pirogov Russian National Research Medical University
Email: cherep.serega@gmail.com
research scientist
Karina Shamil'evna Kardashova
V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: kksh08@yandex.ru
senior researcher
Olga Ivanovna Gurina
V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: olga672@yandex.ru
Head of the laboratory of neurochemistry, Dr. of Medical Sciences, professor of RAS, Corresponding Member of RAS.
Artemiy Sergeevich Silantyev
National Scientific Research Center on Addictions - a branch of the V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation
Email: artsilan@gmail.com
junior researcher
Svetlana Andreevna Pavlova
Institute of Institute of Higher Nervous Activity and Neurophysiology of the Russian Federation
Email: pavlova.sweti@yandex.ru
graduate student
Galina Valerievna Pavlova
Institute of Institute of Higher Nervous Activity and Neurophysiology of the Russian Federation; Sechenov First Moscow State Medical University; «N.N. Burdenko National Medical Research Center of Neurosurgery» of the Ministry of Health of the Russian Federation
Email: lkorochkin@mail.ru
Head of the laboratory; professor of RAS.
Tatiana Alexandrovna Savelieva
Prokhorov General Physics Institute of the Russian Academy of Sciences; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
Email: savelevat@gmail.com
research scientist
Victor Borisovich Loshhenov
Prokhorov General Physics Institute of the Russian Academy of Sciences; National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
Email: loschenov@mail.ru
Head of the Laser Biospectroscopy Laboratory
Vladimir Pavlovich Chekhonin
V.P. Serbsky National Medical Research Centre for Psychiatry and Narcology, Ministry of Health of the Russian Federation; Pirogov Russian National Research Medical University
Email: chekhoninnew@yandex.ru
head of the Department of Fundamental and Applied Neurobiology
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