Еvaluation of immunotropic properties of glyproline compounds in experimental hyperthyroidism

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Abstract

Introduction. Thyroid disease is currently the most common among endocrine pathology. Hyperproduction of thyroid hormones is accompanied by stimulation of humoral reactions, contributing to the development of immune-mediated pathology under certain conditions, which actualizes the need to develop means for correcting neuroimmunoendocrine processes. Gliproline compounds analogous to neuropeptide protein molecules, among which are Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro, can be considered as such agents.

The aim of the study. The aim of the study was to study the immunotropic properties of gliproline compounds Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro under experimental hyperthyroidism.

Material and methods. The immunotropic properties of the gliproline compounds Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro were studied in white nonlinear male rats. Hyperthyroid status in animals was modeled by intragastric administration of L-thyroxine sodium salt pentahydrate («Sigma»; USA) at 150 μg/kg for 21 days. Laboratory animals were divided into groups (n=10): control group – intact animals (control); animals treated with L-thyroxine sodium salt pentahydrate (hyperthyroidism); rats receiving Thr-Lys-Pro-Arg-Pro-Gly-Pro (selanc) and rats receiving Pro-Gly-Pro intraperitoneally daily for 21 days at doses of 87 and 33 μg/kg/day.

Results. The obtained results indicate the presence in gliproline compounds Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro of immune correcting activity manifested under the conditions of experimental hyperthyroidism, with the greatest impact on the humoral unit of immunity, which is confirmed by the presence of statistically significant changes in the cellular parameters of the spleen and titer antierythrocytic antibodies in a direct hemagglutination reaction.

Conclusion. Thus, the study of the immunotropic properties of gliproline peptide compounds under experimental hyperthyroidism established the presence of immune-correcting activity in Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro substances.

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About the authors

Mariyam U. Sergalieva

Astrakhan State Medical University

Author for correspondence.
Email: charlina_astr@mail.ru
ORCID iD: 0000-0002-9630-2913

Associate Professor, Department of Pharmacognosy, Pharmaceutical Technology and Biotechnology, Astrakhan State Medical University of the Ministry of Health of Russia. Candidate of biological sciences

Russian Federation, Bakinskaya St., 121, Astrakhan, 414000

Alexandra A. Tsybizova

Astrakhan State Medical University

Email: sasha3633@yandex.ru
ORCID iD: 0000-0002-9994-4751

Associate Professor, Department of Pharmacognosy, Pharmaceutical Technology and Biotechnology Astrakhan State Medical University of the Ministry of Health of Russia. Candidate of pharmaceutical sciences

Russian Federation, Bakinskaya St., 121, Astrakhan, 414000

Veronika Kh. Murtalieva

Astrakhan State Medical University

Email: murtalieva90@mail.ru
ORCID iD: 0000-0003-0860-4952

Assistant of the Department of Pharmacognosy, Pharmaceutical Technology and Biotechnology Astrakhan State Medical University of the Ministry of Health of Russia

Russian Federation, Bakinskaya St., 121, Astrakhan, 414000

Liudmila A. Andreeva

Institute of Molecular Genetics of the SIC «Kurchatov Institute»

Email: landr@img.ras.ru
ORCID iD: 0000-0002-3927-8590

Deputy Head of the Laboratory of Molecular Pharmacology of Peptides Institute of Molecular Genetics of the National Research Centre «Kurchatov Institute»

Russian Federation, akad. Kurchatov Square, 2, Moscow, 123182

Nikolai F. Myasoedov

Institute of Molecular Genetics of the SIC «Kurchatov Institute»

Email: nfm@img.ras.ru
ORCID iD: 0000-0003-1294-102X

Head of the Laboratory of Molecular Pharmacology of Peptides Institute of Molecular Genetics of the National Research Centre «Kurchatov Institute». Doctor of Chemical Sciences, Professor, academician of the Russian Academy of Sciences

Russian Federation, akad. Kurchatov Square, 2, Moscow, 123182

Marina A. Samotrueva

Astrakhan State Medical University

Email: ms1506@mail.ru
ORCID iD: 0000-0001-5336-4455

Head of the Department of Pharmacognosy, Pharmaceutical Technology and Biotechnology Astrakhan State Medical University of the Ministry of Health of Russia. Doctor of medical sciences, Professor

Russian Federation, Bakinskaya St., 121, Astrakhan, 414000

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2. Effect of Thr-Lys-Pro-Arg-Pro-Gly-Pro and Pro-Gly-Pro compounds on delayed-type hypersensitivity response index and antibody titer in direct hemagglutination response under experimental hyperthyroidism

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