Pathomorphological analysis of salinomycin and nanodiamonds efficacy on Lewis lung carcinoma in mice

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Abstract

Relevance. The search for new antitumor drugs and their selective delivery directly to the tumor site is an important task of modern oncology. For these purposes, currently, the use of various nanoparticles as carriers of medicinal substances is of great importance. The pathomorphological features of tumor cells under the action of salinomycin and nanodiamonds have not been studied enough.

The aim of the work was to study the pathomorphological features of the tumor in mice with transplanted Lewis lung carcinoma, who were treated with the ionoform antibiotic salinomycin and a combination of salinomycin with nanodiamonds.

Material and methods. 20 mice were divided into 4 groups. 1 – control group; 2 – mice received salinomycin; 3 – salinomycin and nanodiamonds; 4 – nanodiamonds. A morphometric study of histological and immunohistochemical tumor preparations stained for PCNA was carried out.

Results. Salinomycin is established to have an antineoplastic action. The use of nanodiamonds did not significantly affect the morphofunctional characteristics of Lewis lung carcinoma and did not change the antitumor activity of salinomycin.

Conclusion. Salinomycin has an antitumor effect and requires further research.

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About the authors

Viktor Vasilyevich Popuchiev

National Medical Research Radiological Сentre of the Ministry of Health of the Russian Federation

Author for correspondence.
Email: popuchiev@mrrc.obninsk.ru
ORCID iD: 0000-0001-9304-7323

A.F. Tsyb Medical Radiological Research Center, Doctor of Medical Sciences, Senior Researcher

Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249036

Elena Mikhailovna Yatsenko

National Medical Research Radiological Сentre of the Ministry of Health of the Russian Federation

Email: yatsenko@mrrc.obninsk.ru
ORCID iD: 0000-0003-0869-0133

A.F. Tsyb Medical Radiological Research Center, Senior Researcher, Candidate of Biological Sciences

Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249036

Natalia Konstantinovna Fomina

National Medical Research Radiological Сentre of the Ministry of Health of the Russian Federation

Email: nkfomina@rambler.ru
ORCID iD: 0000-0002-1499-1349

A.F. Tsyb Medical Radiological Research Center, Senior Researcher, Candidate of Biological Sciences

Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249036

Lyudmila Nikolaevna Mikhina

National Medical Research Radiological Сentre of the Ministry of Health of the Russian Federation

Email: mikhina1976@mail.ru
ORCID iD: 0000-0001-7600-7901

A.F. Tsyb Medical Radiological Research Center, Senior Researcher

Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249036

Leonid Petrovich Zhavoronkov

National Medical Research Radiological Сentre of the Ministry of Health of the Russian Federation

Email: leonid.petrovich@inbox.ru
ORCID iD: 0000-0001-5100-9118

A.F. Tsyb Medical Radiological Research Center, Doctor of Medical Sciences, Professor, Рrofessor of the Scientific and Educational Department

Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249036

Vadim Vasilyevich Yuzhakov

National Medical Research Radiological Сentre of the Ministry of Health of the Russian Federation

Email: ks.med@mail.ru
ORCID iD: 0000-0002-2854-6289

A.F. Tsyb Medical Radiological Research Center, Candidate of Medical Sciences, head of the Laboratory of Radiation Pathomorphology

Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249036

Anatoly Georgievich Konoplyannikov

National Medical Research Radiological Сentre of the Ministry of Health of the Russian Federation

Email: ks.med@mail.ru
ORCID iD: 0000-0003-2766-9030

A.F. Tsyb Medical Radiological Research Center, Doctor of Biological sciences, Professor, head of the Department of Cellular and Experimental Radiation Therapy

Russian Federation, Koroleva str., 4, Obninsk, Kaluga Region, 249036

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2. Fig. 1. Morphology of CLL in mice of the 1st control group: a – histological pattern of the tumor node on day 21 after implantation (scan), hematoxylin and eosin; b – zone of invasive growth of CLL (×200), hematoxylin and eosin; c – zone of solid structure of CLL (×400), hematoxylin and eosin; g – serial section of the CLL to Fig. 1a (scan), immune-stained with PCNA; d – proliferative activity of tumor cells in the growth zone of CLL (×200), PCNA

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3. Fig. 2. Morphology of CLL in group 2 mice that were injected with salinomycin: a – histological picture of the tumor node (scan), stained with hematoxylin and eosin; b – the growth zone of CLL (×200), hematoxylin and eosin; c – zone of solid structure in the proximal part of CLL (×400), hematoxylin and eosin; d – serial section of CLL, immuno-stained with PCNA (scan); e – proliferative activity of tumor cells in the growth zone of CLL (×200), PCNA

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4. Fig. 3. Morphology of CLL in group 3 mice, with the combined use of salinomycin and nanodiamonds: a – histological picture of the tumor node (scan), stained with hematoxylin and eosin; b – the growth zone of CLL (×200), hematoxylin and eosin; c – zone of solid structure in the proximal part of CLL (×400), hematoxylin and eosin; d – serial section of CLL, immuno-stained with PCNA (scan); e – immuno-staining of tumor cell nuclei on PCNA in the growth zone of CLL (×200), PCNA

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5. Fig. 4. Morphology of CLL in group 4 mice treated with nanodiamonds: a – histological picture of the tumor node (scan), hematoxylin and eosin; b – vascularization of the CLL growth zone (×200), hematoxylin and eosin; c – zone of solid structure in the proximal part of CLL (×400), hematoxylin and eosin; d – serial section of CLL, immuno-stained with PCNA (scan); e – proliferative activity of tumor cells in the growth zone of CLL (×200), PCNA

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