Salivary biomarkers in adolescents with type 1 diabetes mellitus (pilot study)
- Authors: Shcherbakova M.M.1, Morozova N.S.1, Allahverdiev A.F.1, Lizunova I.Y.1, Saushkina A.A.1, Zaharova N.B.2
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Affiliations:
- Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
- Federal State Budgetary Educational Institution of Higher Education “Saratov State Medical University named after V.I.Razumovsky”, Healthcare Ministry of the Russian Federation
- Issue: Vol 23, No 2 (2025)
- Pages: 32-37
- Section: Original research
- URL: https://journals.eco-vector.com/1728-2918/article/view/678673
- DOI: https://doi.org/10.29296/24999490-2025-02-04
- ID: 678673
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Abstract
Introduction. Type 1 diabetes mellitus is a chronic autoimmune disease associated with severe complications of organs and systems, including complications of the course of dental diseases.
Material and methods. Results of 55 patients with type 1 DM became the material of laboratory-diagnostic studies. Patients with type 1 diabetes were divided into 2 groups depending on blood glucose levels: Group 1 (n=28) – patients with glucose levels ≥7 mmol/l; Group 2 (n=27) – patients with glucose levels ≤7 mmol/l; Control group – 28 patients. Periodontal tissues were evaluated by indices: PMA, SBI. OHI-S index reflected the state of oral hygiene. Mucosal immunity of oral fluid was studied using ELISA analysis of pro- and anti-inflammatory cytokines IL-8, IL-10, IL-1β, MCP-1, TNF-α, VEGF-А.
Results. The content in unstimulated oral fluid of IL-1β in patients with type 1 DM was 84.1 (95–74.6) pg/mL in the group with glucose ≤ 7 mmol/L, 80.1 (115.4–64.9) pg/mL in the group with glucose ≥7 mmol/L, 23.1 (28.2–18.6) pg/mL in the comparison group, VEGF-A was 1340 (1545–967.5) pg/mL, 1170 (1517.5–954.8) pg/mL in group 2, and 1655 (1860–1370.5) pg/mL in group 3. TNF-α was higher in group 1 – 9.8 (11.4–7.5) pg/mL than in group 2 – 8.3 (10.3–6.6) pg/mL and in group 3 – 1 (1.1–0.9) pg/mL (p < 0.05).
Conclusion. ROC curve analysis showed that these biomarkers have better sensitivity and specificity. The increase of IL-8, TNF-α, IL-1β content in mixed saliva up to 285.5 (339.8–214.5) pg/ml, 9.8 (11.4–7.5), 84.1 (95–74.6) pg/ml, respectively, was characterized by high medians of OHI-s, PMA and SBI indices, associated with high activity of inflammatory-destructive changes in periodontal tissues and impaired metabolic control of glucose level.
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About the authors
Maria Maksimovna Shcherbakova
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Author for correspondence.
Email: marya.scherbakova@yandex.ru
ORCID iD: 0009-0006-1816-7839
5th year student, Department of propaedeutics of dental diseases, E.V. Borovsky Institute of Dentistry
Russian Federation, Trubetskaya str., 8, build. 2, Moscow, 119991Natalia Sergeevna Morozova
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: morozova_n_s_2@staff.sechenov.ru
ORCID iD: 0000-0002-6453-1615
SPIN-code: 4654-9842
Doctor of Medical Sciences, Professor of the Department of Propaedeutics of the Department of Propaedeutics of Stomatologic Diseases, E.V. Borovsky Institute of Dentistry
Russian Federation, Trubetskaya str., 8, build. 2, Moscow, 119991Arif Fuad ogly Allahverdiev
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: arif_17_03_1998@mail.ru
ORCID iD: 0009-0000-9209-3803
Department Applicant of Propaedeutics of Dental Diseases, E.V. Borovsky Institute of Dentistry
Russian Federation, Trubetskaya str., 8, build. 2, Moscow, 119991Irina Yuryevna Lizunova
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: i.lizun0va@yandex.ru
ORCID iD: 0009-0008-2470-8667
5th year student
Russian Federation, Trubetskaya str., 8, build. 2, Moscow, 119991Alina Alexandrovna Saushkina
Federal State Autonomous Educational Institution of Higher Education I.M.Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Email: saushkina.alina@mail.ru
ORCID iD: 0009-0007-4654-6905
5th year student
Russian Federation, Trubetskaya str., 8, build. 2, Moscow, 119991Natalia Borisovna Zaharova
Federal State Budgetary Educational Institution of Higher Education “Saratov State Medical University named after V.I.Razumovsky”, Healthcare Ministry of the Russian Federation
Email: lipidgormon@mail.ru
ORCID iD: 0000-0001-9410-2240
SPIN-code: 5354-6327
Doctor of Medical Sciences, Professor, Department of Biochemistry and Clinical Laboratory Diagnostics
Russian Federation, Bol'shaya Kazach'ya str., 112, Saratov, 410012References
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