Prognostic significance of CD68+macrophages in autoimmune inflammation in experimental study
- Authors: Dmitrieva M.L.1, Tikhonovskaya O.A.1, Logvinov S.V.1, Mustafina L.R.1, Petrov I.A.1, Bordunova E.G.1, Samoilova I.G.1,2, Kudlay D.A.2,3,4
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Affiliations:
- FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation
- Novosibirsk National Research State University
- Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
- National Research Center – Institute of Immunology Federal Medical-Biological Agency of Russia
- Issue: Vol 23, No 4 (2025)
- Pages: 43-48
- Section: Original research
- URL: https://journals.eco-vector.com/1728-2918/article/view/689166
- DOI: https://doi.org/10.29296/24999490-2025-04-07
- ID: 689166
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Abstract
Introduction. Autoimmune oophoritis is one of the causes of premature ovarian insufficiency in 3–40% of cases. Representative diagnosis is possible only through histological examination of ovarian biopsy material, which complicates the identification of early stages of the pathological process when changes may be reversible.
Objective: to study the number and localization of antigen-presenting cells (macrophages) expressing CD68 and determine their role in the pathogenesis of autoimmune inflammation during the formation of experimental autoimmune oophoritis and experimental bacterial chronic inflammation of uterine appendages.
Material and methods. The study was conducted on 45 mature outbred female rats divided into 3 groups: main group with autoimmune oophoritis model (n=25), comparison group with bacterial chronic inflammation of uterine appendages model (n=10), and control group (n=10). Histological examination of ovaries, immunohistochemical staining with CD68 antibodies, and determination of anti-ovarian antibodies in blood serum were performed.
Results. In autoimmune oophoritis, high CD68 expression was revealed on the surface of M1 phenotype tissue macrophages. Maximum numerical density of CD68+macrophages was reached by day 15 of the experiment (714.62 cells/mm2), while in bacterial inflammation by day 60 (492.84 cells/mm2). CD68+macrophages migrate from medulla to cortex, surrounding growing follicles. By day 30, a decrease in the number of growing follicles and an increase in atretic bodies were noted with elevated anti-ovarian antibody concentrations to 10.3–14.1 ng/ml.
Conclusion. Macrophages with increased CD68+expression may serve as promising early markers of premature ovarian insufficiency of autoimmune etiology, which is of interest for clinical practice.
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About the authors
Margarita Leonidovna Dmitrieva
FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation
Author for correspondence.
Email: dmitrieva.ml@ssmu.ru
ORCID iD: 0000-0002-2958-9424
SPIN-code: 3312-5500
Candidate of Medical Sciences, Associate Professor, Associate Professor of the Department of Obstetrics and Gynecology
Russian Federation, Moskovsky tract, 2, Tomsk, 634050Olga Anatolyevna Tikhonovskaya
FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation
Email: tikhonovskaya.oa@ssmu.ru
ORCID iD: 0000-0003-4309-5831
SPIN-code: 2458-0890
Doctor of Medical Sciences, Professor of the Department of Obstetrics and Gynecology
Russian Federation, Moskovsky tract, 2, Tomsk, 634050Sergey Valentinovich Logvinov
FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation
Email: logvinov.sv@ssmu.ru
ORCID iD: 0000-0002-9876-6957
SPIN-code: 3998-9015
Doctor of Medical Sciences, Professor, Head of the Department of Histology, Embryology and Cytology
Russian Federation, Moskovsky tract, 2, Tomsk, 634050Liliya Ramilyevna Mustafina
FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation
Email: lrmustafina@yandex.ru
ORCID iD: 0000-0003-3526-7875
SPIN-code: 1070-7905
Doctor of Medical Sciences, Professor of the Department of Histology, Embryology and Cytology
Russian Federation, Moskovsky tract, 2, Tomsk, 634050, Russian FederationIlya Alekseevich Petrov
FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation
Email: petrov.ia@ssmu.ru
ORCID iD: 0000-0002-0697-3896
SPIN-code: 8356-5503
Doctor of Medical Sciences, Professor of the Department of Obstetrics and Gynecology, Head of the Assisted Reproductive Technologies Center
Russian Federation, Moskovsky tract, 2, Tomsk, 634050Ekaterina Grigor’evna Bordunova
FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation
Email: katabordunova6@gmail.com
ORCID iD: 0009-0001-6951-9391
3th year student of the pediatric faculty
Russian Federation, Moskovsky tract, 2, Tomsk, 634050Iuliia Gennadievna Samoilova
FSFEI HE “Siberian State Medical University” of the Ministry of Health of the Russian Federation; Novosibirsk National Research State University
Email: samoilova_y@inbox.ru
ORCID iD: 0000-0002-2667-4842
SPIN-code: 8644-8043
Doctor of Medical Sciences, Professor of the Department of Faculty Therapy with Clinical Pharmacology Course, Head of the Department of Pediatrics with Endocrinology Course, Director of the Institute of Medicine and Medical Technologies
Russian Federation, Moskovsky tract, 2, Tomsk, 634050; Pirogova str., 1, Novosibirsk, 630090Dmitry Anatolyevich Kudlay
Novosibirsk National Research State University; Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University); National Research Center – Institute of Immunology Federal Medical-Biological Agency of Russia
Email: dakudlay@generium.ru
ORCID iD: 0000-0003-1878-4467
SPIN-code: 4129-7880
Doctor of Medical Sciences, Corresponding Member of the Russian Academy of Sciences, Director of Innovative Development Programs, Professor, Department of Pharmacology at the Institute of Pharmacy, Leading Researcher, Laboratory of Personalized Medicine and Molecular Immunology No.71
Russian Federation, Pirogova str., 1, Novosibirsk, 630090; Trubetskaya st., 8, build. 2, Moscow, 119991; Kashirskoe shosse, 24, Moscow, 115522References
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