STRUCTURALLY FUNCTIONAL ORGANIZATION OF THE GENOME OF A CLINICALLY SIGNIFICANT STRAIN HELICOBACTER PYLORI HP42K ISOLATED FROM THE PATIENTA HOSPITAL IN THE BELARUS REPUBLIC


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Introduction. The use of genomic approaches allows a detailed studying of the pathogenetic potential of H. pylori bacteria. The aim of the study. To investigate the genome structural and functional organization of the H. pylori isolated in the Republic of Belarus. Methods. Using next-generation sequencing with the Ion PGM System genome analyzer, a genomic analysis of the clinically significant H. pylori isolate was performed. Results. The study of the structural and functional organization of the H. pylori HP42K isolate genome can identify the pathogenetic and antigenic determinants as possible predictors of the clinical properties. The loci: five DNA motifs AATAAAGATA and EPIYA-ABC motifs in the structure of the cagA gene, as well as the oipA gene, and alleles s1 and m1a of the vacA gene were identified to be not related to carcinogenesis. Three circular sequences represented by chromosomal and plasmid DNA of H. pylori were identified and deposited in GenBank NCBI with identification numbers CP034314.1, CP034313.1, and CP034312.1. Conclusion. The analysis of the H. pylori HP42K isolate genome showed its pathogenetic features to be associated with the development of gastroduodenal pathology but not associated with stomach cancer risk.

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作者简介

E. Voropaev

Gomel State Medical University

Email: e.voropaev@gsmu.by
Gomel, Republic of Belarus

O. Baranov

Gomel State Medical University

Gomel, Republic of Belarus

L. Valentovich

Institute of Microbiology of the National Academy of Sciences of Belarus

Minsk, Republic of Belarus

O. Osipkina

Gomel State Medical University

Gomel, Republic of Belarus

A. Zyatkov

Gomel State Medical University

Gomel, Republic of Belarus

N. Bonda

Gomel Regional Center of Hygiene, Epidemiology and Public Health

Gomel, Republic of Belarus

A. Voropaeva

Gomel State Medical University

Gomel, Republic of Belarus

E. Platoshkin

Gomel State Medical University

Gomel, Republic of Belarus

V. Mizura

Gomel State Medical University

Gomel, Republic of Belarus

A. Shaforost

Gomel State Medical University

Gomel, Republic of Belarus

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