Mitochondria - intracellular targets of geroprotective effect of V007

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Introduction. Oxidative stress and mitochondrial dysfunction are observed in virtually any disease associated with premature aging. Signaling molecules produced by mitochondria can act as specific targets for the action of pharmacological agents in order to regulate the processes of cellular aging, which opens up new possibilities for the search and development of drugs for effective pharmacotherapy of age-associated pathologies conditions. The aim of the study. To study the effect of V007 on the expression of mitochondrial biomarkers in rat liver cells in vivo to elucidate the possible mechanism of its geroprotective action. Methods. Molecular biological methods were used to study the expression of key mitochondrial proteins: Tom70, Tom20, VDAC, DRP1, prohibitin, Parkin, PINK1 in liver cells of young and old rats in normal conditions and with the use of the innovative drug V007. Results. The data obtained indicate that when using the V007preparation, there is a statistically significant increase in the expression of all studied mitochondrial proteins in hepatocytes of old rats to the level of young animals: DRP1 by 1.3 times; Parkin 1.2 times; PINK11.2 times; Prohibitin 1.4 times; Tom 201.3 times; Tom 701.2 times; VDAC 1.2 times. This change in the expression of mitochondrial proteins is due to the activation of their synthesis and increased activity of the mitochondrial transport systems. Conclusion. V007has a general regulatory and geroprotective effect, being a targeted pharmacological agent that regulates the functions of hepatocytes during aging by normalizing the expression of key mitochondrial proteins.

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作者简介

Igor Kvetnoy

Iva Pharm LLC; St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

编辑信件的主要联系方式.
Email: info@spbniif.ru
Head of the Center of Molecular Biomedicine

Ekaterina Mironova

Iva Pharm LLC; St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

Email: katerina.mironova@gerontology.ru
Senior Researcher, Center of Molecular Biomedicine

Yulia Krylova

St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

Email: info@spbniif.ru
Senior Researcher, Center of Molecular Biomedicine

Anastasia Mylnikova

St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

Email: info@spbniif.ru
laboratory assistant researcher, Center of Molecular Biomedicine

Tatiana Zubareva

St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

Email: info@spbniif.ru
Senior Researcher, Center of Molecular Biomedicine

Daria Leont’eva

St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

Email: info@spbniif.ru
Research assistant, Center of Molecular Biomedicine

Victoria Polyakova

St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

Email: info@spbniif.ru
Leading Research Fellow, Center of Molecular Biomedicine

Mariya Petrosyan

St. Petersburg Research Institute of Phthisiopulmonology of the Ministry of Health of the Russian Federation

Email: mariya@labpharm.spb.ru
Senior Researcher, Center of Molecular Biomedicine

参考

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2. Fig. 1. Relative area of expression of mitochondrial proteins in the liver of rats Note. * – p<0.05 compared to the group of young animals (control); ** – p<0.05 compared to the group of old animals.

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3. Fig. 2. Immunofluorescent staining with antibodies to Tom70 of liver cells of rats. A – young animals (control group), ×1000; B – old animals, ×1000; C – application of the drug V007, ×1000.

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