Antibiotic resistance of strains of gram-positive cocci isolated from prostate secretion in men with chronic bacterial prostatitis

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access


Objective: to characterize the antibiotic resistance of gram-positive cocci strains isolated from the prostate secretion in men with chronic bacterial prostatitis at the level of phenotype and genotype. Materials and methods. Bacteria were isolated from the prostate secretion of men of reproductive age (20-45 years) with chronic bacterial prostatitis by conventional bacteriological method. The type of microorganisms was determined by MALDI-TOF mass spectrometry. Resistance to 16 antibiotics of 31 strains of E. faecalis and 91 cultures of coagulase-negative staphylococci (CoNS) was determined by the disk diffusion method. Antibiotic resistance genes (mecA; blaZ; aac(6’)-aph (2’’); ant (4’)-Ia; aph (3’)-IIIa; gyrA, grlA) were detected using polymerase chain reaction (PCR ) using selected primers. Results. A high resistance of enterococci to antibacterial drugs was revealed: fluoroquinolones, carbapenens, cephalosporins (with the exception of cefoperazone), gentamicin and oxacillin. It has been established that CoNS are characterized by variable antibiotic resistance, while: isolates of S. epidermidis and S. haemolyticus are resistant to all studied fluoroquinolones and carbapenems; S. warneri to carbapenems and the vast majority of studied cephalosporins; S. saprophyticus - to aminoglycosides. Amoxiclav and cefoperazone are characterized by the highest activity against clinical isolates. Using PCR, the presence of genetic determinants of resistance to aminoglycosides and ß-lactams was established in the isolates, with a predominance of the studied genes in CoNS. Conclusion. For effective antibiotic therapy in chronic bacterial prostatitis, it is necessary to conduct regional monitoring of the resistance of microorganisms to antibacterial drugs.

Full Text

Restricted Access

About the authors

O. L Kartashova

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Dr. Biol. Sci., docent, leading researcher of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

O. A Pashinina

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

PhD in Biol. Sci., senior scientific of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

N. V Morozova

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

PhD in Biol. Sci., researcher of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

T. M Pashkova

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Dr. Biol. Sci., leading researcher of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

M. D Kuzmin

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Dr. Med. Sci., leading researcher laboratories for studying the mechanisms of formation of human microbial biocenoses; urologist of the highest qualification category, consultant of GAUZ «OOKB», Honored Doctor of the Russian Federation and the Republic of Mordovia Orenburg, Russia


  1. Божедомов В.А. Современные возможности лечения хронического простатита. Обзорная статья. Андрология и генитальная хирургия. 2016;17(3):10-22.
  2. Holt J.D., Garrett W.A., McCurry T.K., Teichman J.M.Common questions about chronic prostatitis: a systematic review. Am. Fam. Physician. 2016;93(4):29-0296 / PMID: 26926816
  3. Коган М.И., Набока Ю.Л., Исмаилов Р.С., Белоусов И.И., Гудима И.А. Бактериальный простатит: эпидемиология и этиология. Урология. 2018;6:144-148). Doi:
  4. Ибишев Х.С., Набока Ю.Л., Мамедов Э.А., Крайний П.А., Манцев А.А., Коган М.И. Резистентность к антибактериальным препаратам у пациентов с хроническим рецидивирующим бактериальным простатитом. Материалы XXI конгресса Российского общества урологов. Урология. 2021; Приложение 5:461-462.
  5. Maslak E., Mista W., Zloch M. et al., A new approach to imaging and rapid microbiome identification for prostate cancer patients. Biomedicines. 2022;10:1806. Doi: 10.339/biomedicines10081806.
  6. Cai T., Mazzoli S., Meacci F. Epidemiological features and resistance pattern in uropathogens isolated from chronic bacterial prostatitis. J. Microbiol.2011;49(3):448-454. doi: 10.1007/s12275-011-0391-z.
  7. Stamatiou K., Magri V., Perletti G. et al. Gram-positive microorganisms isolated during chronic bacterial prostatitis investigation. A retrospective study. Hellenic Urology. 2019;30(4):35-49.
  8. Медведев В.Л., Ефремов М.Е. Эффективность применения препарат аденопросин в комплексном лечении пациентов с хроническим бактериальным простатитом. Инновационная медицина Кубани. 2020;3(19):45-50.
  9. Коган М.И., Набока Ю.Л., Исмаилов Р.С. Микробиота секрета простаты: сравнительный анализ хронического простатита категорий II и IIIA. Урология. 2020;2:16-22). Doi:
  10. Heras-Canas V., Gutierrez-Soto B., Serrato-Garcia M.L. et al. Chronic bacterial prostatitis. Clinical and microbiological study of 331 cases. Med. Clin. 2016.19;147(4):144-147.
  11. Локшин К.Л. Актуальные вопросы лечения бактериальных и абактериальных простатитов: каковы показания для антибактериальной и фитотерапии? Урология и нефрология. 2016;4:6-9.
  12. Alberto Trinchieri, Khalid Mohammed Abdelrahman, Kamran Hassan Bhatti, Jibril O. Bello, Krishanu Das, Ognyan Gatsev, Ivanka Gergova, Vittorio Magri, Nikos Mourmouras, Panagiotis Mourmouris, Soni Murdeshwar. Spectrum of Causative Pathogens and Resistance Rates to Antibacterial Agents in Bacterial Prostatitis. Diagnostics. 2021;11:1333. Doi:
  13. Litwin M.S., McNaughton-Collins M., Fowler FJ Jr, Nickel JC, Calhoun E.A., Pontari M.A., Alexander R.B., Farrar J.T., O’Leary M.P. The National Institutes of Health chronic prostatitis symptom index: development and validation of a new outcome measure. Chronic Prostatitis Collaborative Research Network. J Urol. 1999;162:369-375. doi: 10.1016/s0022-5347(05)68562-х.
  14. Rosen R.C., Cappelleri J.C., Smith M.D., Lipsky J., Pena B.M. Development and evaluation of an abridged, 5-item version of the International Index of Erectile Function (IIEF-5) as a diagnostic tool for erectile dysfunction.Int J Impot Res. 1999;11(6):319-326. doi: 10.1038/sj.ijir.3900472.
  15. ГОСТ Р 52379-2005 Надлежащая клиническая практика. Москва: Стандартинформ, 2006;33 с.
  16. МУК 4.2.1890-04 Определение чувствительности микроорганизмов к антибактериальным препаратам. М.: Минздрав России, 2005;62 c.
  17. Gostev V., Kruglov A., Kalinogorskaya O., Dmitrenko O., Khokhlova O.E., Yamamoto T., Lobzin Y., Ryabchenko I., Sidorenko S. Molecular epidemiology and antibiotic resistance of methicillin-resistant Staphylococcus aureus circulating in the Russian Federation. Infection, Genetics and Evolution. 2017;53:189-194.
  18. Лакин Г.Ф. Биометрия. М.: Высшая школа,1990; 352 с.
  19. Божедомов В.А. Хронический простатит: новая парадигма лечения. Урология. 2016;3:78-90.
  20. Stamatiou K., Pierris N. Mounting resistance of uropathogens to antimicrobial agents: A retrospective study in patients with chronic bacterial prostatitis relapse. Department of Urology, Tzaneio General Hospital of Piraeus, Piraeu Investig Clin Urol 2017;58:271-280. Doi:
  21. Chow J.W. Aminoglycoside resistance in enterococci. Clin Infect Dis. 2000; 31(2):586-589. Doi: 10.1086/313949' target='_blank'>https://doi: 10.1086/313949.
  22. Amini F., Krimpour H.A., Ghaderi M., Vaziri S., Ferdowsi S., Azizi M. Prevalence of aminoglycoside resistance genes in Enterococcus strains in Kermanshah, Iran. Iran J Med Sci. 2018; 43(5):487-493.
  23. Abdelkareem M.Z., Sayed M., Hassuna N.A., Mahmoud M.S., Abdelwahab S.F. Multi-drug-resistant Enterococcus faecalis among Egyptian patients with urinary tract infection. J Chemotherapy. 2017;29(2):74-82. doi: 10.1080/1120009X.2016.182358.
  24. Zhu L.X., Zhang Z.W., Wang C., Yang H.W., Jiang D., Zhang Q., Mitchelson K., Cheng J. Use of a DNA microarray for simultaneous detection of antibiotic resistance genes among staphylococcal clinical isolates. J. Clin. Microbiol. 2007;45:3514-3521. doi: 10.1128/JCM.02340-06.
  25. Шаркова В.А., Лайман Е.Ф., Мазур М.Е. Генетически обусловленная патогенность и антибиотикорезистентность штаммов Staphylococcus spp. Тихоокеанский медицинский журнал. 2014;3:46-49.
  26. Jung-Whan Chon, Un Jung Lee, Ryan Bensen, Stephanie West, Angel Paredes, Jinhee Lim, Saeed Khan, Mark E. Hart, K. Scott Phillips, Kidon Sung. Virulence Characteristics of mecA-Positive Multidrug-Resistant Clinical Coagulase-Negative Staphylococci. Microorganisms. 2020;8:659. doi: 10.3390/microorganisms8050659/.
  27. Зайцев А.А., Карпов О.И., Сидоренко С.В. Стафилококки и ванкомицин: тенденции и противостояния. Антибиотики и химиотерапия. 2003;48(6):20-26.
  28. Miller W.R., Munita J.M., Arias C.A. Mechanisms of antibiotic resistance in enterococci. Expert Rev Anti Infect Ther. 2014;12(10):1221-1236. Doi: 10.1586/ 14787210.2014.956092.
  29. Коменкова Т.С., Зайцева Е.А. Современные представления о механизмах резистентности к антимикробным препаратам Enterococcus faecalis и Enterococcus faecium. Антибиотики и химиотерапия. 2020;65(11-12): 38-48. Doi:
  30. Partridge Sally R., Kwong Stephen M., Firth Neville, Jensen Slade O. Mobile Genetic Elements Associated with Antimicrobial Resistance. Clin Microbiol Rev. 2018;31(4 doi: 10.1128/CMR.00088-17.

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies