Antibiotic resistance of strains of gram-positive cocci isolated from prostate secretion in men with chronic bacterial prostatitis

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Abstract

Objective: to characterize the antibiotic resistance of gram-positive cocci strains isolated from the prostate secretion in men with chronic bacterial prostatitis at the level of phenotype and genotype. Materials and methods. Bacteria were isolated from the prostate secretion of men of reproductive age (20-45 years) with chronic bacterial prostatitis by conventional bacteriological method. The type of microorganisms was determined by MALDI-TOF mass spectrometry. Resistance to 16 antibiotics of 31 strains of E. faecalis and 91 cultures of coagulase-negative staphylococci (CoNS) was determined by the disk diffusion method. Antibiotic resistance genes (mecA; blaZ; aac(6’)-aph (2’’); ant (4’)-Ia; aph (3’)-IIIa; gyrA, grlA) were detected using polymerase chain reaction (PCR ) using selected primers. Results. A high resistance of enterococci to antibacterial drugs was revealed: fluoroquinolones, carbapenens, cephalosporins (with the exception of cefoperazone), gentamicin and oxacillin. It has been established that CoNS are characterized by variable antibiotic resistance, while: isolates of S. epidermidis and S. haemolyticus are resistant to all studied fluoroquinolones and carbapenems; S. warneri to carbapenems and the vast majority of studied cephalosporins; S. saprophyticus - to aminoglycosides. Amoxiclav and cefoperazone are characterized by the highest activity against clinical isolates. Using PCR, the presence of genetic determinants of resistance to aminoglycosides and ß-lactams was established in the isolates, with a predominance of the studied genes in CoNS. Conclusion. For effective antibiotic therapy in chronic bacterial prostatitis, it is necessary to conduct regional monitoring of the resistance of microorganisms to antibacterial drugs.

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About the authors

O. L Kartashova

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Email: labpersist@mail.ru
Dr. Biol. Sci., docent, leading researcher of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

O. A Pashinina

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Email: olga25mikro@mail.ru
PhD in Biol. Sci., senior scientific of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

N. V Morozova

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Email: natascha210994@mail.ru
PhD in Biol. Sci., researcher of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

T. M Pashkova

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Email: pashkova070782@mail.ru
Dr. Biol. Sci., leading researcher of laboratories of persistence and symbiosis of microorganisms Orenburg, Russia

M. D Kuzmin

Institute of Cellular and Intracellular Symbiosis UB of the Russian Academy of Sciences

Email: mdk-49@mail.ru
Dr. Med. Sci., leading researcher laboratories for studying the mechanisms of formation of human microbial biocenoses; urologist of the highest qualification category, consultant of GAUZ «OOKB», Honored Doctor of the Russian Federation and the Republic of Mordovia Orenburg, Russia

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