PLGA mesh-collagen hybrid scaffold and tissue-engineered product in substitution urethroplasty: experimental validation


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Abstract

Urethral strictures are a pressing issue in modern medicine. Substitution urethroplasty is considered one of the most effective treatment methods. However, despite the surgery showing good results, many problems remain unresolved, one being substitute material deficiency in extensive or recurrent strictures, as well as in cases requiring multistage surgeries, including those used to treat hypospadias. Graft removal also leaves the donor area prone to diseases and increases the length of surgery leading to a higher risk of intra- and postoperative complications. Tissue engineering (namely tissue-engineered products comprised of scaffolds and cells) may be a useful tool in dealing with these issues. The authors assessed the characteristics of a novel hybrid scaffold created from “reconstructed” collagen and a poly(lactic-co-glycolic acid) mesh. The resulting composite product showed good mechanical properties and functional performance. The hybrid scaffold was non-cytotoxic and provided an adequate base for cell adhesion and proliferation. Biodegradation resulted in the scaffold being replaced by urothelium and urethral mucosa. The newly formed tissues possessed adequate structural and functional properties. Only one rabbit out of 12 developed urethral stricture at the site of scaffold implantation. The abovementioned facts suggest that the novel hybrid scaffold is a promising tissue-engineered product with potential implication in substitution urethroplasty.

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About the authors

P. V Glybochko

Sechenov First Moscow State Medical University

Yu. G Alyaev

Sechenov First Moscow State Medical University

Department of Urology, Faculty of Medicine

A. B Shekhter

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

A. Z Vinarov

Sechenov First Moscow State Medical University

Research Institute for Uronephrology and Reproductive Health

L. P Istranov

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

E. V Istranova

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

R. K Aboyants

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

A. V Lyundup

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

M. E Krasheninnikov

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

A. E Guller

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

D. V Butnaru

Sechenov First Moscow State Medical University

Email: butnaru_dv@mail.ru
Deputy Director, Research Institute for Uronephrology

L. V Marisov

Sechenov First Moscow State Medical University

Research Institute for Uronephrology and Reproductive Health

D. F Kantimerov

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

A. V Kurkov

Sechenov First Moscow State Medical University

Research Institute for Uronephrology and Reproductive Health

E. I Safronova

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

E. A Vorob’eva

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

M. M Iritsyan

Sechenov First Moscow State Medical University

Research Institute for Molecular Medicine

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