Investigation of mechanisms of action of growth factors of autologous platelet-rich plasma used to treat erectile dysfunction
- Authors: Epifanova M.V1, Chalyi M.E2, Krasnov A.O3
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Affiliations:
- Research Institute for Uronephrology and Human Reproductive Health, I.M. Sechenov First MSMU of Minzdrav of Russia (Sechenov University)
- I.M. Sechenov First MSMU
- I.M. Sechenov First MSMU of Minzdrav of Russia (Sechenov University)
- Issue: No 4 (2017)
- Pages: 46-49
- Section: Articles
- URL: https://journals.eco-vector.com/1728-2985/article/view/289342
- DOI: https://doi.org/10.18565/urol.2017.4.46-48
- ID: 289342
Cite item
Open Access
Access granted
Abstract
Aim. To determine the quantitative and qualitative composition of growth factors (PDGF-AA, PDGF-BB, VEGF, VEGF-D, FGF-acid, FGF-basic) and platelets in various modifications of APRP. Materials and methods. Blood of 12malevolunteers(controlgroup)and 12 patients with ED was used to prepare APRP and the subsequently determine the concentration of growth factors. The growth factor concentrations (FGF acid, FGF basic, PDGF-AA, PDGF-BB, VEGF, VEGF-D) was determined using a flow cytometry-based xMAP Fuminex (Gen-Probe) system. Results. Concentration of platelets in APRP obtained by two stage centrifugation, reached 1480 (1120-1644) in the control group and 1232 (956-1502) in patients with ED. The concentration of growth factors in the samples prepared without preliminary freezing was: PDGF-AA 842 (22-3700), PDGF-BB 2837 (1460-4100), FGF-basic 7.9 (0.28-127), FGF-acid 3, 4 (0.14-11), VEGF 19 (4.6-46), VEGF-D 21 (14-38). After thawing, the concentration of all growth factors in the samples increased. Discussion. The study findings suggest that the mechanism of erectile function recovery following the use of APRP is through the active substances detected in APRP, i.e. FGF-basic, PDGF-AA, PDGF-BB, VEGF, VEGF-D and FGF-acid. Also, the study showed that the content of growth factors in APRP after of freezing/thawing is higher than in APRP that has not been frozen. This is due to the cell membrane destruction at extremely low temperatures during freezing.
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About the authors
M. V Epifanova
Research Institute for Uronephrology and Human Reproductive Health, I.M. Sechenov First MSMU of Minzdrav of Russia (Sechenov University)
Email: epifanova_maya@mail.ru
Ph.D., heading Researcher at the Research Institute for Uronephrology and Human Reproductive Health
M. E Chalyi
I.M. Sechenov First MSMU
Email: chalyy@bk.ru
Dr.Med.Sci., Prof., Director of CIEP “Medicine of the Future»
A. O Krasnov
I.M. Sechenov First MSMU of Minzdrav of Russia (Sechenov University)
Email: krasnov_92@inbox.ru
clinical resident at the Department of Urology
References
- Marx R.E., Carlson E.R., Eichstaedt R.M., Schimmele S.R., Strauss J.E., Georgeff K.R. Platelet-rich plasma: Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1998;85(6):638-646.
- Marx RE. Platelet-rich plasma (PRP): what is PRP and what is not PRP? Implant Dent. 2001;10(4):225.
- Chalyj M.E., Grigorjan V.A., Epifanova M.V., Krasnov A.O. The effectiveness of intracavernous autologous platelet-rich plasma in the treatment of erectile dysfunction. Urologiia. 2015;(4):76-79.
- Glybochko P.V., Chaly M.E., Yepifanova M.V., Akhvlediani N.D., Krasnov A.O. Application of autoplasma enriched with platelet derived growth factor in the treatment of erectile dysfunction. Urologiia. 2015;(1):100-103.
- Ehrenfest D., Rasmusson L., Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte and platelet-rich fibrin (L-PRF). Trends Biotechnol. 2009;27(3):158-167.
- Xie D., Pippen A.M., Odronic S.I., Annex B.H., Donatucci C.F. Intracavernosal basic fibroblast growth factor improves vasoreactivity in the hypercholesterolemic rabbit. J Sex Med. 2006;3(2):223-232.
- Dai Q., Silverstein A.D., Davies M.G., Hagen P.O., Donatucci C.F., A nnex B.H. Systemic basic fibroblast growth factor induces favorable histological changes in the corpus cavernosum of hypercholesterolemic rabbits. J Urol. 2003;170(2 Pt 1):664-668.
- Wu Y.N., Wu C.C., Sheu M.T., Chen K.C., Ho H.O., Chiang H.S. Optimization of platelet-rich plasma and its effects on the recovery of erectile function after bilateral cavernous nerve injury in a rat model. J Tissue Eng Regen Med. 2016;10(10):E294-E304.
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