Comparative analysis of antibiotic therapy and cytokine therapy (the drug “superlymph”) for male infertility associated with male accessory gland infections

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Abstract

Aim. To compare the effects of cytokine and antibacterial therapy on semen parameters and additional functional tests in patients with infertility due to male accessory gland infections (MAGI) who are preparing for assisted reproductive technologies (ART).

Materials and methods. A randomized, prospective, controlled clinical trial without blinding was carried out. A total of 60 men from infertile couples with MAGI who were preparing to ART was included in the study. In the main group (n=30) patients received Superlymph, 1 suppository of 25 units, 2 times a day for 20 days. In the control group (n=30), the antibacterial drug Doxycycline 100 mg 2 times a day for 28 days was given. After the end of therapy on day 33±3, a repeated sperm analysis, MAR test, a test for reactive oxygen species in the ejaculate and sperm DNA fragmentation, and bacteriological examination of sperm was performed. In addition, a survey for adverse events was carried out. The significance of differences in initial and final parameters and differences between groups was assessed using the Student’s t-test, Wilcoxon test and Mann-Whitney U-test depending on the data distribution. The Shapiro-Wilk test was done to investigate the normality of data distribution. Fisher's exact test was used to compare categorical variables.

Results. The final analysis included data from 53 patients (n=28 in the main group and n=25 in the control group). In both groups, a significant decrease in the level of free oxygen radicals in the ejaculate was noted (p=0.031), which was more pronounced in the main group. There were no differences in other semen parameters. Eradication of the microorganism according to bacteriological examination occurred in 57.1% of patients in the main group and in 88% of those in the control group (p=0.016). In patients receiving monotherapy with Superlymph, there was a significant decrease in the sperm DNA fragmentation index and the concentration of leukocytes in the ejaculate. In patients receiving antibacterial therapy, there was a significant increase in ejaculate volume, a decrease in the proportion of IgG-associated sperm and leukocyte concentration.

Conclusion. Many issues of diagnosis and treatment of MAGI have not been thoroughly studied and are poorly standardized. Considering the problems of increasing antibiotic resistance, alternative treatment options are needed. Cytokine therapy (the drug Superlymph) is an effective alternative method of monotherapy for male infertility due to MAGI and is optimal for quickly preparing a couple for ART protocol, given its positive effect on oxidative stress and the index of sperm DNA fragmentation. The prospect of combination therapy with antibiotics and Superlymph seems promising.

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About the authors

S. I. Gamidov

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov of the Ministry of Health of Russia; I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russia (Sechenov University)

Author for correspondence.
Email: safargamidov@yandex.ru
Scopus Author ID: 0000-0002-9128-2714

Ph.D., MD, professor, Chief of the Department of Andrology and Urology, professor at the Department of Obstetrics, Gynecology, Perinatology and Reproductology

Russian Federation, Moscow; Moscow

A. Yu. Popova

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov of the Ministry of Health of Russia

Email: a_popova@oparina4.ru
ORCID iD: 0000-0003-1163-5602

Ph.D., senior researcher at the Department of Andrology and Urology

Russian Federation, Moscow

T. V. Shatylko

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov of the Ministry of Health of Russia

Email: moscowandrology@gmail.com
ORCID iD: 0000-0002-3902-9236

Ph.D., urologist at the Department of Andrology and Urology

Russian Federation, Moscow

K. I. Li

National Medical Research Center of Obstetrics, Gynecology and Perinatology named after V.I. Kulakov of the Ministry of Health of Russia

Email: kostya_lee_1991@mail.ru
ORCID iD: 0000-0001-5925-5647

a candidate for an academic degree at the Department of Andrology and Urology

Russian Federation, Moscow

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