Analysis of expression of MGP, UMOD, SPP1, F2, FN1, HAVCR1 genes associated with the development of urolithiasis

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Abstract

Introduction. Improving molecular genetic methods for diagnosing polygenic forms of urolithiasis is an urgent task in urology.

Aim. To study the relative expression level of the MGP, UMOD, SPP1, F2, FN1, HAVCR1 genes associated with the development of urolithiasis and the number of proteins encoded by them (Gla protein, prothrombin, fibronectin, osteopontin, uromodulin and TIM-1 protein) in patients with urolithiasis.

Material and methods. Forty-nine people were examined, including 45 patients with urolithiasis and 4 people from the control group, with the average age was 67±12 years. Healthy volunteers served as controls. mRNA was isolated from peripheral venous blood. It was reverse-transcribed into cDNA, and the relative expression levels of the target genes were quantified by RT-PCR.

Results. According to the level of expression of the genes MGP, UMOD, SPP1, F2, FN1, HAVCR1 in the blood, clear hiatuses were identified between the values in the control group and in those with urolithiasis, while no differences were found in the quantitative content of their protein products, either in the blood or urine.

Conclusion. In the blood, with a relative level of expression of the gene MGP above 10%, F2 above 0.5%, FN1 above 0.2%, UMOD above 0.04%, SPP1 above 0.001%, HAVCR1 above 0.01%, urolithiasis can be predicted. The specified levels of expression of the genes MGP, UMOD, SPP1, F2, FN1, HAVCR1 in the blood allow reliable identification of patients with urolithiasis in comparison with the quantitative content of protein products of these genes in the blood and urine.

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About the authors

Dmitry N. Khotko

FGBOU VO “Saratov State University named after V.I. Razumovsky” of Ministry of Health of Russia

Author for correspondence.
Email: dnksar@list.ru
ORCID iD: 0000-0002-7966-5181

Ph.D., Head of the Department of Urology, senior researcher at the Research Institute of Fundamental and Clinical Uronephrology 

Russian Federation, 410012, Saratov, Bolshaya Kazachya St., 112

Natalya V. Polukonova

FGBOU VO “Saratov State University named after V.I. Razumovsky” of Ministry of Health of Russia

Email: polukonovanv@yandex.ru
ORCID iD: 0000-0001-9228-6808

Doctor of Biological Sciences, professor, Department of General Biology, Pharmacognosy and Botany

Russian Federation, 410012, Saratov, Bolshaya Kazachya St., 112

Timofey E. Pylaev

FGBOU VO “Saratov State University named after V.I. Razumovsky” of Ministry of Health of Russia

Email: pylaev.te@staff.sgmu.ru
ORCID iD: 0000-0002-2701-3333

Ph.D., Director of the Center for Molecular Genetic and Cellular Technologies

Russian Federation, 410012, Saratov, Bolshaya Kazachya St., 112

Sergey S. Veretennikov

FGBOU VO “Saratov State University named after V.I. Razumovsky” of Ministry of Health of Russia

Email: dnksar@list.ru

junior researcher, Center for Molecular Genetic and Cellular Technologies

Russian Federation, 410012, Saratov, Bolshaya Kazachya St., 112

Alla B. Bucharskaya

FGBOU VO “Saratov State University named after V.I. Razumovsky” of Ministry of Health of Russia

Email: allaalla_72@mail.ru
ORCID iD: 0000-0003-0503-6486

D.Sc., Head of the Center for Collective Use of EO, Deputy Director of the Research Institute for Research

Russian Federation, 410012, Saratov, Bolshaya Kazachya St., 112

Vladimir M. Popkov

FGBOU VO “Saratov State University named after V.I. Razumovsky” of Ministry of Health of Russia

Email: popkov.vm@staff.sgmu.ru
ORCID iD: 0000-0003-2876-9607

Ph.D., MD, Head of the Department of Urology 

Russian Federation, 410012, Saratov, Bolshaya Kazachya St., 112

Anastasia I. Khotko

FGBOU VO “Saratov State University named after V.I. Razumovsky” of Ministry of Health of Russia

Email: pivovarova-nastya@ya.ru
ORCID iD: 0000-0002-4569-9906

Ph.D., assistant of the Department of Urology 

Russian Federation, 410012, Saratov, Bolshaya Kazachya St., 112

Artem I. Tarasenko

I.M. Sechenov First Moscow State Medical University of the Ministry of Health of Russia (Sechenov University)

Email: tar-art@yandex.ru
ORCID iD: 0000-0002-3258-8174

Ph.D., Deputy Director for Innovative Development of the Institute for Urology and Human Reproductive Health

Russian Federation, Bolshaya Pirogovskaya str., 2, building 1. Moscow, 119991

Ramil R. Altynbaev

State Healthcare Institution "Regional Clinical Oncology Dispensary"

Email: r.r.altinbaev@ya.ru
ORCID iD: 0009-0005-8438-368X

Head of the Department of Oncourology

Russian Federation, 110053, Saratov, Smirnovskoe Gorge microdistrict, bldg. 1B

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