Study of influenza A virus nuclear export protein aggregates using atomic force microscopy

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Abstract

The nuclear export protein (NEP) plays an important role in intracellular processes during infection of host cells. In this paper, aggregates of various morphologies and sizes were identified and characterized for the first time using atomic force microscopy. The results obtained may be used for development of new antiviral drugs and developing of NEP-based drug delivery systems.

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About the authors

Е. V. Dubrovin

Lomonosov Moscow State University; Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency

Author for correspondence.
Email: dubrovin@polly.phys.msu.ru
ORCID iD: 0000-0001-8883-5966

Doct. of Sci. (Physics and Mathematics), Leading Researcher

Russian Federation, Moscow; Moscow

О. N. Koroleva

Lomonosov Moscow State University

Email: dubrovin@polly.phys.msu.ru
ORCID iD: 0009-0001-2359-0723

Cand. of Sci. (Chemistry), Senior Researcher

Russian Federation, Moscow

N. V. Kuzmina

A.N. Frumkin Institute of Physical Chemistry and Electrochemistry

Email: dubrovin@polly.phys.msu.ru
ORCID iD: 0009-0003-3585-8066

Cand. of Sci. (Physics and Mathematics), Researcher

Russian Federation, Moscow

V. L. Drutsa

Moscow State University, A.N.Belozersky Institute of Physico-Chemical Biology

Email: dubrovin@polly.phys.msu.ru
ORCID iD: 0009-0003-7780-9182

Cand. of Sci. (Chemistry), Leading Researcher

Russian Federation, Moscow

References

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  9. Dubrovin E.V., Koroleva O.N., Golovko A.O., Kuzmina N.V., Klinov D.V., Drutsa V.L. Atomic Force Microscopy Investigation of Influenza A Virus Nuclear Export Protein Aggregation, Microscopy and Microanalysis. 2019. Vol. 25. PP. 1342–1343. https://doi.org/10.1017/S143192761900744X
  10. Koroleva O.N., Dubrovin E.V., Tolstova A.P., Kuzmina N.V., Laptinskaya T.V., Yaminsky I.V., Drutsa V.L. A hypothetical hierarchical mechanism of the self-assembly of the Escherichia coli RNA polymerase σ7° subunit, Soft Matter. 2016. Vol. 12. PP. 1974–1982. https://doi.org/10.1039/C5SM02934A

Supplementary files

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2. Fig.1. AFM images of protein NEP-1 protein aggregates deposited from the solution on the surface of freshly cleaved mica

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3. Fig.2. Histograms of distribution: a – heights; b – volumes of NEP-1 protein aggregates

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Copyright (c) 2024 Dubrovin Е.V., Koroleva О.N., Kuzmina N.V., Drutsa V.L.