Comprehensive evaluation of the effectiveness of the human placenta hydrolyzate in the correction of the age-associated facial skin changes

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

Background. Age-associated facial skin changes are not only an aesthetic but also a social problem, especially for women. The key markers of skin aging include a decrease in regenerative potential, impaired barrier function, and loss of elasticity. With age, the proliferative and metabolic activity of dermal fibroblasts decreases, and structural and compositional remodeling of skin extracellular matrix proteins, primarily collagen, occurs. Human placenta hydrolyzate (HPH)-based peptide preparations are currently used for therapeutic purposes in various branches of medicine.

Objective. Comprehensive (clinical-instrumental, immunological, microbiological, immunohistochemical) evaluation of the effectiveness of the use of the HPH preparation for the correction of age-associated facial skin modifications.

Methods. The study included 25 women aged 39 to 59 years with signs of age-related facial skin changes. All women received five pharmacopuncture intramuscular injections of the drug Laennec into the projection of biologically active points of the face 2 ml per procedure 1 time in 5 days.

Results. After a course of five intramuscular injections of the HPH preparation into biologically active points of the face, a significant decrease in the depth of wrinkles in the paraorbital and perioral zones, the degree of deformation of the facial oval, an increase in skin hydration and bactericidal activity was noted. In the peripheral blood, a significant increase in the number of regulatory T-lymphocytes and monocytes was observed with a simultaneous increase in the activity and intensity of phagocytosis of the latter; the concentration of pro-inflammatory IL-6 and -8 decreased with a parallel increase in the IL-4 level. According to the mmunohistochemical analysis of the skin in the dermis, the bulk density of collagen-I and -III, laminin, FGF-2, TGF-β, VEGF, IL-1α, -6, -20 increased most significantly with a simultaneous decrease in PDGF and IL-8; there was an increase in the TGF-β, EGF levels and a decrease in IGF level in the epidermis.

Conclusion. Thus, the HPH preparation activates dermal fibroblasts, supports the renewal and trophism of epidermal cells, and restores the biomechanical and bactericidal properties of aging skin.

Full Text

Restricted Access

About the authors

E. K. Kuznetsova

Orenburg State Medical University

Email: alena_mez_75@mail.ru
Russian Federation, Orenburg

Elena A. Mezentseva

South Ural State Medical University

Author for correspondence.
Email: alena_mez_75@mail.ru

Cand. Sci. (Med.), Associate Professor of the Department of Microbiology, Virology and Immunology

Russian Federation, Chelyabinsk

Yu. V. Kudrevich

South Ural State Medical University

Email: alena_mez_75@mail.ru
Russian Federation, Chelyabinsk

I. I. Dolgushin

South Ural State Medical University

Email: alena_mez_75@mail.ru
Russian Federation, Chelyabinsk

O. R. Ziganshin

South Ural State Medical University

Email: alena_mez_75@mail.ru
Russian Federation, Chelyabinsk

T. A. Zayats

Chelyabinsk Regional Pathological Anatomical Bureau

Email: alena_mez_75@mail.ru
Russian Federation, Chelyabinsk

K. V. Nikushkina

South Ural State Medical University

Email: alena_mez_75@mail.ru
Russian Federation, Chelyabinsk

References

  1. Мантурова Н.Е., Городилов Р.В., Кононов А.В. Старение кожи: механизмы формирования и структурные изменения. Анналы пластической, реконструктивной и эстетической хирургии. 2010;1:88–92. [Manturova N.E., Gorodilov R.V., Kononov A.V. Skin aging: formation mechanisms and structural changes. Annaly plasticheskoi, rekonstruktivnoi i esteti-cheskoi khirurgii. 2010;1:88–92. (In Russ.)].
  2. Trojahn C., Dobos G., Lichterfeld A., et al. Characterizing Facial Skin Ageing in Humans: Disentangling Extrinsic from Intrinsic Biological Phenomena. BioMed Res Int. 2015;2015:318586. doi: 10.1155/2015/318586.
  3. Sole-Boldo L., Raddatz G., Schutz S., et al. Single-cell transcriptomes of the human skin reveal age-related loss of fibroblast priming. Communicat Biol. 2020;3(1):188. doi: 10.1038/s42003-020-0922-4.
  4. Lee H., Hong Y., Kim M. Structural and Functional Changes and Possible Molecular Mechanisms in Aged Skin. Int J Mol Sci. 2021;22(22):12489. doi: 10.3390/ijms222212489.
  5. Cao C., Xiao Z., Wu Y., Ge C. Diet and Skin Aging – From the Perspective of Food Nutrition. Nutrients. 2020;12(3):870. doi: 10.3390/nu12030870.
  6. Franceschi C., Bonafe M., Valensin S., et al. Inflamm-aging. An evolutionary perspective on immunosenescence. Ann New York Acad Sci. 2000;908(1):244–54. doi: 10.1111/j.1749-6632.2000.tb06651.x.
  7. Franceschi C., Garagnani P., Parini P., et al. Inflammaging: a new immune-metabolic viewpoint for age-related diseases. Nat Rev Endocrinol. 2018;14:576–90. doi: 10.1038/s41574-018-0059-4.
  8. Артемьева О.В., Ганковская Л.В. Воспа-лительное старение как основа возраст-ассоциированной патологии. Медицинская иммунология. 2020;22(3):419–32. [Artem’eva O.V., Gankovskaya L.V. Inflammatory aging as the basis of age-associated pathology. Meditsinskaya immunologiya. 2020;22(3):419–32. (In Russ.)].
  9. Chambers E.S., Vukmanovic-Stejic M. Skin barrier immunity and ageing. Immunol. 2019;160(2):116–25. doi: 10.1111/imm.13152.
  10. Pan S.Y., Chan M.K.S., Wong M.B.F., et al. Placental therapy: An insight to their biological and therapeutic properties. J Med Ther. 2017;1(3):1–6.
  11. Pogozhykh O., Prokopyuk V., Figueiredo C., Pogozhykh D. Placenta and Placental Derivatives in Regenerative Therapies: Experimental Studies, History, and Prospects. Stem Cell Int. 2018;2018:4837930. doi: 10.1155/2018/4837930.
  12. Phonchai R., Naigowit P., Ubonsaen B., et al. Improvement of Atrophic Acne Scar and Skin Complexity by Combination of Aqueous Human Placenta Extract and Mesenchymal Stem Cell Mesotherapy. J Cosmet Dermatol Sci Applicat. 2020;10(1):1–7.
  13. Громова О.А., Торшин И.Ю., Гилельс А.В. и др. Препараты плаценты человека: фундаментальные и клинические исследования. Врач. 2014;4:67–72. Gromova O.A., Torshin I.Yu., Gilel’s A.V. et al. Human Placenta Preparations: Fundamental and Clinical Research. Vrach. 2014;4:67–72. (In Russ.)].
  14. Торшин И.Ю., Громова О.А. Мировой опыт использования гидролизатов плаценты человека в терапии. Экспериментальная и клиническая гастроэнтерология. 2019;10:79–89. [Torshin I.Yu., Gromova O.A. World experience in the use of human placenta hydrolysates in therapy. Eksperimental’naya i klinicheskaya gastroenterologiya. 2019;10:79–89. (In Russ.)].
  15. Максимов В.А., Каримова И.М. Возможности плацентарной медицины в восстановительном лечении. Вестник восстановительной медицины. 2018;83(1):32–7. [Maksimov V.A., Karimova I.M. Possibilities of placental medicine in rehabilitation treatment. Vestnik vosstanovitel’noi meditsiny. 2018;83(1):32–7. (In Russ.)].
  16. Кошелева И., Каримова И. Плацентарная терапия в anti-age медицине и косметологии. Les Nouvell Esthetiques. 2017;3:2–3. [Kosheleva I., Karimova I. Placental therapy in anti-age medicine and cosmetology. Les Nouvell Esthetiques. 2017;3:2–3. (In Russ.)].
  17. Торшин И.Ю., Згода В.Г., Громова О.А. и др. Анализ легкой пептидной фракции Лаеннека методами современной протеомики. Фармакокинетика и фармакодинамика. 2016;4:31–42. [Torshin I.Yu., Zgoda V.G., Gromova O.A. et al. Analysis of the Laennec light peptide fraction by modern proteomics. Farmakokinetika i farmakodinamika. 2016;4:31–42. (In Russ.)].
  18. Каримова И. Клинические исследования эффективности применения препарата Лаеннек в дерматологии и эстетической медицине. Инъекционные методы в косметологии. 2010;4:38–40. [Karimova I. Clinical studies of the effectiveness of Laennec in dermatology and aesthetic medicine. In»ektsionnye metody v kosmetologii. 2010;4:38–40. (In Russ.)].
  19. Громова О.А., Торшин И.Ю., Диброва Е.А. и др. Мировой опыт применения препаратов из плаценты человека: результаты клинических и экспериментальных исследований. Обзор. Пластическая хирургия и косметология. 2011;3:525–36. [Gromova O.A., Torshin I.Yu., Dibrova E.A. et al. World experience in the use of drugs from human placenta: results of clinical and experimental studies. Review. Plasticheskaya khirurgiya i kosmetologiya. 2011;3:525–36. (In Russ.)].
  20. Гилельс А.В., Демидов В.И., Жидоморов Н.Ю. и др. Эффективность воздействия экстрактов плаценты человека на пигментообразование кожи на примере препаратов Лаеннек и Курасен. Эффективная фармакотерапия. 2013;36:40–7. [Gilel’s A.V., Demidov V.I., Zhidomorov N.Yu. et al. Efficiency of human placenta extracts influence on skin pigment formation by the example of Laennec and Kurasen preparations. Effektivnaya farmakoterapiya. 2013;36:40–7. (In Russ.)].
  21. Лучина Е.Н. Возможности применения препарата Лаеннек в лечении рубцовых изменений кожи. Экспериментальная и клиническая дерматокосметология. 2012;4:35–9. [Luchina E.N. Possibilities of using Laennec in the treatment of cicatricial changes in the skin. Eksperimental’naya i klinicheskaya dermatokosmetologiya. 2012;4:35–9. (In Russ.)].
  22. Стенько А., Гилельс А., Течиева С. и др. Применение плацентарного препарата «Лаеннек» в комплексной терапии рубцовых изменений кожи. Эстетическая медицина. 2014;XIII(3):3–7. [Sten’ko A., Gilel’s A., Techieva S. et al. The use of the placental preparation «Laennec» in the complex therapy of cicatricial skin changes. Esteticheskaya meditsina. 2014;XIII(3):3–7. (In Russ.)].
  23. Круглова Л.С., Талыбова А.П., Стенько А.Г. Комбинированное применение лазеротерапии и фармафореза в лечении атрофических рубцов. Кремлевская медицина. Клинический вестник. 2016;4:93–8. [Kruglova L.S., Talybova A.P., Sten’ko A.G. Combined use of laser therapy and pharmacophoresis in the treatment of atrophic scars. Kremlevskaya meditsina. Klinicheskii vestnik. 2016;4:93–8. (In Russ.)].
  24. Леонов С.В., Марусич Е.И., Громова О.А. и др. Антивозрастной эффект гидролизата плаценты человека. Доказательный стандарт. Терапия. 2017;4(14):130–38. [Leonov S.V., Marusich E.I., Gromova O.A. Anti-aging effect of human placenta hydrolyzate. Evidence standard. Therapy. 2017;4(14):130–38. (In Russ.)].
  25. Долгушин И.И., Андреева Ю.С., Савочкина А.Ю. Нейтрофильные внеклеточные ловушки и методы оценки функционального статуса нейтрофилов. М., 2009. 208 с. [Dolgushin I.I., Andreeva Yu.S., Savochkina A.Yu. Neutrophil extracellular traps and methods for assessing the functional status of neutrophils. M., 2009. 208 p. (In Russ.)].
  26. Новикова Л.В., Лебедева К.М., Яковлева Э.М. и др. Иммунологические методы исследования: учебное пособие. Саранск: Мордовский государственный университет им. Н.П. Огарева. 1981. 92 с. [Novikova L.V., Lebedeva K.M., Yakovleva E.M. and other Immunological research methods: a tutorial. Saransk: Mordovia State University n.a. N.P. Ogaryov. 1981. 92 p. (In Russ.)].
  27. Liu K., Taiichi K., Kobayashi Y., et al. Anti–aging effect of Laennec injection (Human Placental Extract) on normal adults. Clin Pharmacol Ther. 2004;14(3):259–65.
  28. Hibino S. The practice of the placenta medication in anti–aging medical treatment. J Japan Associat Adult Orthodont. 2008;15(2):69.
  29. Торшин И., Громова О., Диброва Е. и др. Влияние препарата Лаеннек на маркеры старения. Эстетическая медицина. 2017;XVI(2):1–11. [Torshin I., Gromova O., Dibrova E. et al. Effect of Laennec on aging markers. Esteticheskaya meditsina. 2017;XVI(2):1–11. (In Russ.)].
  30. Борзых О.Б., Шнайдер Н.А., Карпова Е.И. и др. Синтез коллагена в коже, его функциональные и структурные особенности. Медицинский вестник Северного Кавказа. 2021;16(4):443–50. [Borzykh O.B., Shnaider N.A., Karpova E.I. et al. Synthesis of collagen in the skin, its functional and structural features. Meditsinskii vestnik Severnogo Kavkaza. 2021;16(4):443–50. (In Russ.)].
  31. Shin J., Kwon S., Choi J., et al. Molecular Mechanisms of Dermal Aging and Antiaging Approaches. Int J Mol Sci. 2019;20(9):2126. doi: 10.3390/ijms20092126.
  32. Зорина А., Зорин В., Черкасов В. Дермальные фибробласты: разнообразие фенотипов и физиологических функций, роль в старении кожи. Эстетическая медицина. 2012;XI(1):15–31. [Zorina A., Zorin V., Cherkasov V. Dermal fibroblasts: diversity of phenotypes and physiological functions, role in skin aging. Esteticheskaya meditsina. 2012;XI(1):15–31. (In Russ.)].
  33. Roig-Rosello E., Rousselle P. The Human Epidermal Basement Membrane: A Shaped and Cell Instructive Platform That Aging Slowly Alters. Biomolecul. 2020;10:1607. doi: 10.3390/biom10121607.
  34. Рукша Т.Г., Аксененко М.Б., Климина Г.М., Новикова Л.В. Внеклеточный матрикс кожи: роль в развитии дерматологических заболеваний. Вестник дерматологии и венерологии. 2013;6:32–9. [Ruksha T.G., Aksenenko M.B., Klimina G.M., Novikova L.V. Extracellular matrix of the skin: role in the development of dermatological diseases. Vestnik dermatologii i vene-rologii. 2013;6:32–9. (In Russ.)].
  35. Kim D., Kim S.Y., Mun S.K., et al. Epidermal growth factor improves the migration and contractility of aged fibroblasts cultured on 3D collagen matrices. Int J Mol Med. 2015;35(4):1017–25. doi: 10.3892/ijmm.2015.2088.
  36. Симбирцев А.С. Иммунофармакологические аспекты системы цитокинов. Бюллетень сибирской медицины. 2019;18(1):84–95. [Simbirtsev A.S. Immunopharmacological aspects of the cytokine system. Byulleten’ sibirskoi meditsiny. 2019;18(1):84–95. (In Russ.)].
  37. De Araujo R., Lobo M., Trindade K., et al. Fibroblast Growth Factors: A Controlling Mechanism of Skin Aging. Skin Pharmacol Physiol. 2019;32:275–82. doi: 10.1159/000501145.
  38. Yang L., Zhang D., Wu H., et al. Basic Fibroblast Growth Factor Influences Epidermal Homeostasis of Living Skin Equivalents through Affecting Fibroblast Phenotypes and Functions. Skin Pharmacol Physiol. 2018;31(5):229–37. doi: 10.1159/000488992.
  39. Juhl P., Bondesen S., Hawkins C.L., et al. Dermal fibroblasts have different extracellular matrix profiles induced by TGF-β, PDGF and IL-6 in a model for skin fibrosis. Sci Rep. 2020;1:17300. doi: 10.1038/s41598-020-74179-6.
  40. Болотная Л.А., Сербина И.М., Сариан Е.И. Сосудистый эндотелиальный фактор роста и его патогенетическое значение при заболеваниях кожи. Дерматовенерология. Косметология. Сексопатология. 2011;1–4:88–94. [Bolotnaya L.A., Serbina I.M., Sarian E.I. Vascular endothelial growth factor and its pathogenetic significance in skin diseases. Dermatovenerologiya. Kosmetologiya. Seksopatologiya. 2011;1–4:88–94. (In Russ.)].
  41. Атькова Е.Л., Рейн Д.А., Ярцев В.Д., Суббот А.М. Влияние цитокина TGF-β и других факторов на процесс регенерации. Вестник офтальмологии. 2017;4:89–96. [At’kova E.L., Rein D.A., Yartsev V.D., Subbot A.M. Influence of TGF-β cytokine and other factors on the regeneration process. Vestnik oftal’mologii. 2017;4:89–96. (In Russ.)].
  42. Taniguchi K., Arima K., Masuoka M., et al. Periostin Controls Keratinocyte Proliferation and Differentiation by Interacting with the Paracrine IL-1a/IL-6 Loop. J Invest Dermatol. 2014;134(5):1295–304. doi: 10.1038/jid.2013.500.
  43. Johnson B.Z., Stevenson A.W., Prele C.M., et al. The Role of IL-6 in Skin Fibrosis and Cutaneous Wound Healing. Biomed. 2020;8(5):101. doi: 10.3390/biomedicines8050101.
  44. Dufour A.M., Alvarez M., Russo B., Chizzolini C. Interleukin-6 and Type-I Collagen Production by Systemic Sclerosis Fibroblasts Are Differentially Regulated by Interleukin-17A in the Presence of Transforming Growth Factor-Beta 1. Front Immunol. 2018;9:1865. doi: 10.3389/fimmu.2018.01865.
  45. Пелипенко Л.В., Сергиенко А.В., Ивашев М.Н. Эффекты трансформирующего фактора роста бета-1. Международный журнал экспериментального образования. 2015;3–5:558–59. [Pelipenko L.V., Sergienko A.V., Ivashev M.N. Effects of transforming growth factor beta-1. Mezhdunarodnyi zhurnal eksperimental’nogo obrazovaniya. 2015;3–5:558–59. (In Russ.)].
  46. Сарбаева Н.Н., Пономарева Ю.В., Миляко- ва М.Н. Макрофаги: разнообразие фенотипов и функций, взаимодействие с чужеродными материалами. Гены и клетки. 2016;11(1):9–17. [Sarbaeva N.N., Ponomareva Yu.V., Milyakova M.N. Macrophages: variety of phenotypes and functions, interaction with foreign materials. Geny i kletki. 2016;11(1):9–17. (In Russ.)].
  47. Romano M., Fanelli G., Tan N., et al. Expanded Regulatory T Cells Induce Alternatively Activated Monocytes With a Reduced Capacity to Expand T Helper-17 Cells. Front Immunol. 2018;9:1625. doi: 10.3389/fimmu.2018.01625.
  48. Kanno Y., Shu E., Niwa H., et al. Alternatively activated macrophages are associated with the α2AP production that occurs with the development of dermal fibrosis. Arthr Res Ther. 2020;22:76. doi: 10.1186/s13075-020-02159-2.
  49. Morikawa M., Derynck R., Miyazono K. TGF-β and the TGF-β Family: Context-Dependent Roles in Cell and Tissue Physiology. The Biology of the TGF-β Family. Ed. by Derynck R., Miyazono K. Cold Spring Harbor Laboratory Press, 2017. 1164 p.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies