Physical properties of surfactant as a basis for its effectiveness in the experiment

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Abstract

The article presents data on our experimental studies devoted to the effects of different concentrations of poractant alpha (80 mg/ml and 40 mg/ml) on its distribution in a biological lung model. Neutral aqueous ink served as a marker, for which the surfactant was a conductor solution. The homogeneity of the surfactant distribution could be judged by visual observation, as well as pathological examination of macro- and micropreparations. Preliminary, the viscosity of surfactants, the fluidity of poractant alpha (PA) were analyzed in laboratory conditions, and capillary phenomena were studied using PA with different concentrations. In laboratory conditions, the PA solution with a phospholipid concentration of 40 mg/ml demonstrated the lowest viscosity and, as a result, higher fluidity than PA 80 mg/ml. The surfactant with a lower viscosity was more evenly distributed in the biological model of rabbit lungs, which improved their straightening, which required lower mechanical ventilation parameters, and was combined with less severe damage to the alveolar tissue.

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About the authors

Aleksei V. Mostovoi

Vorokhobov City Clinical Hospital No. 67 of the Moscow Healthcare Department; Russian Medical Academy of Continuous Professional Education; Yaroslavl State Medical University

Author for correspondence.
Email: valmost@mail.ru
ORCID iD: 0000-0002-7040-9683

Cand. Sci. (Med.), Head of the Neonatal Intensive Care Unit, Associate Professor, Department of Neonatology named after V.V. Gavryushov, Assistant, Department of Outpatient Therapy, Clinical Laboratory Diagnostics and Medical Biochemistry, Faculty of Postgraduate Education

Russian Federation, Moscow; Moscow; Yaroslavl

Anna L. Karpova

Vorokhobov City Clinical Hospital No. 67 of the Moscow Healthcare Department; Russian Medical Academy of Continuous Professional Education; Yaroslavl State Medical University

Email: anna1409@mail.ru
ORCID iD: 0000-0002-1024-0230

Cand. Sci. (Med.), Head of Neonatal Department, Associate Professor, Department of Neonatology named after V.V. Gavryushov, Assistant, Department of Outpatient Therapy, Clinical Laboratory Diagnostics and Medical Biochemistry, Faculty of Postgraduate Education

Russian Federation, Moscow; Moscow; Yaroslavl

Roman A. Burenkov

Kaluga Regional Children’s Clinical Hospital

Email: burenkov.kodb@gmail.com
ORCID iD: 0000-0002-4102-0644

Head of Department, Pathologist

Russian Federation, Kaluga

Petr B. Ermolinsky

Lomonosov Moscow State University

Email: valmost@mail.ru
ORCID iD: 0000-0002-4688-2307

Researcher, Laboratory of Biomedical Photonics, Faculty of Physics

Russian Federation, Moscow

Andrey E. Lugovtsov

Lomonosov Moscow State University

Email: anlug1@gmail.com
ORCID iD: 0000-0001-5222-8267

Senior Researcher, Laboratory of Biomedical Photonics, Faculty of Physics

Russian Federation, Moscow

Svetlana V. Vorobyeva

Vorokhobov City Clinical Hospital No. 67 of the Moscow Healthcare Department

Email: Svetilnikvv@mail.ru
ORCID iD: 0009-0006-8472-1201

Anesthesiologist-Intensivist

Russian Federation, Moscow

Daria R. Sinitsa

Vorokhobov City Clinical Hospital No. 67 of the Moscow Healthcare Department

Email: sinitsadaria@yandex.ru
ORCID iD: 0009-0004-7083-4171

Anesthesiologist-Intensivist

Russian Federation, Moscow

Irina M. Igina

Vorokhobov City Clinical Hospital No. 67 of the Moscow Healthcare Department

Email: ign.irn@mail.ru
ORCID iD: 0000-0002-7733-4258

Anesthesiologist-Intensivist

Russian Federation, Moscow

Sergei A. Nekhoroshkin

Russian Medical Academy of Continuous Professional Education

Email: nekhoroshkin2000@gmail.com
ORCID iD: 0009-0006-3851-5247

Physician, Clinical Resident, Department of Neonatology named after V.V. Gavryushov

Russian Federation, Moscow

Nikolai N. Volodin

Dmitry Rogachev National Medical Research Center for Pediatric Hematology, Oncology and Immunology

Email: 0209vnn@mail.ru
ORCID iD: 0000-0002-2667-8229

Dr. Sci. (Med.), Professor, Full Member of the Russian Academy of Sciences, President of the Russian Association of Perinatal Medicine Specialists; Head of the Department of Pediatrics

Russian Federation, Moscow

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Supplementary files

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2. Figure 1. Rotational viscometer RM100 CP1000 PLUS (Lamy Rheology Instruments, France)

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3. Figure 2. Evaluation of capillary phenomena of poractant alpha solution with a concentration of 80 mg/mL (left) and with a concentration of 40 mg/mL (right) using a capillary with an internal hole diameter of 1.2 mm and a length of 120 mm

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4. Figure 3. The degree of fluidity of a surfactant solution with different concentrations

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5. Figure 4. Distribution of surfactant viscosity at a shear rate of 1000 s-1

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6. Figure 5. Model №1, on the left - X-ray control of the endotracheal tube position, on the right - the first ink spot appears within the first minutes after the introduction of stained surfactant on the cardiac side of the left lung

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7. Figure 6. Model №2, on the left - X-ray control of the endotracheal tube position, on the right - the first ink spots appear within the first minutes after the introduction of stained surfactant evenly on the surface of both lungs from the diaphragmatic surface

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8. Figure 7. Rabbit lungs after removal from the chest cavity (view from the dorsal side of the lungs). Tracheal sections were made at the level of the end of the endotracheal tube. A more uniform distribution of stained surfactant throughout the lungs is determined in Model №2 (Model №2)

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9. Figure 8. Macroscopic preparations of layered lung tissue sections with a step of 0.5 cm demonstrate complete staining of the lungs from the inside (formalin-fixed lung preparations). L1 - left lung of Model №1, R1 - right lung of Model №1, L2 - left lung of Model №2, R2 - right lung of Model №2

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10. Figure 9. Model №1, on the left in various areas of the lungs (1a, 1b) pronounced dystelectasis and atelectasis are determined. Model №2, on the right are areas (2a, 2b) with uniform straightening of the lungs an hour after the introduction of surfactant and artificial ventilation

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