The effect of mesenchymal stromal cells on the restoration of the structure of the vocal folds with cicatricial changes. Controlled pilot study


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Background. Cicatricial lesions of the vocal folds are one of the most clinically significant among non-life-threatening diseases of the larynx leading to permanent loss of voice. Currently, there is no method to fully restore the normal structure and vibrational characteristics of the vocal folds and, accordingly, the voice function in cicatricial lesions. Objective. The evaluation of morphology of the vocal folds with cicatricial lesions after the introduction of autologous mesenchymal stromal cells (MSCs). Methods. The mature cicatricial process in the vocal folds was chosen as the basis of the experimental model. As laboratory animals, 10 laboratory rabbits were used. They were divided into two groups, and underwent surgical removal of the middle third of the right vocal fold. 90 days after the first surgery, a second intervention was performed: the scar of the vocal fold within the visible borders was excised and saline was injected into the defect in the first experimental group, and suspensions of autologous MSCs from the bone marrow reserve in the second group. In both groups, the evaluation of the results of the experiment was carried out 90 days after the second intervention (180 days from the start of the experiment). Evaluation of the results of the experiment was carried out using optical microscopy of histological preparations with various staining. Results. The study showed a significant regenerative potential of MSCs when they were introduced into the secondary wound of the vocal fold. The most interesting changes were observed in the collagen structures of scar tissue. After cell therapy, scars had a smaller thickness of collagen fibers, their microarchitectonics was represented by a significantly less dense stacking with a longitudinal and mutually parallel orientation compared to the random arrangement of collagen fibrils in scars without the introduction of MSCs. Conclusion. Implantation of MSCs in the wound of the vocal folds after scar removal contributes to their more complete regeneration. MSCs can be considered as a possible source of cell therapy for patients with cicatricial damage to the vocal folds. The reasonability of further research in this direction is justified.

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作者简介

V. Svistushkin

Sechenov First Moscow State Medical University (Sechenov University)

Department of Ear, Nose and Throat Diseases, N.V. Sklifosovsky Institute of Clinical Medicine Moscow, Russia

S. Starostina

Sechenov First Moscow State Medical University (Sechenov University)

Department of Ear, Nose and Throat Diseases, N.V. Sklifosovsky Institute of Clinical Medicine Moscow, Russia

Mikhail Svistushkin

Sechenov First Moscow State Medical University (Sechenov University)

Teaching Assistant of the Department of Ear, Nose and Throat Diseases, N.V. Sklifosovsky Institute of Clinical Medicine; Teaching Assistant of the Department of Ear, Nose and Throat Diseases Moscow, Russia

S. Timashev

National Research Nuclear University MEPhI

Moscow, Russia

A. Shekhter

Sechenov First Moscow State Medical University (Sechenov University)

Institute for Regenerative Medicine, Scientific and Technological Park of Biomedicine Moscow, Russia

A. Fayzullin

Sechenov First Moscow State Medical University (Sechenov University)

Institute for Regenerative Medicine, Scientific and Technological Park of Biomedicine Moscow, Russia

A. Pobivantseva

Sechenov First Moscow State Medical University (Sechenov University)

Department of Ear, Nose and Throat Diseases, N.V. Sklifosovsky Institute of Clinical Medicine Moscow, Russia

A. Lunicheva

Sechenov First Moscow State Medical University (Sechenov University)

Department of Ear, Nose and Throat Diseases, N.V. Sklifosovsky Institute of Clinical Medicine Moscow, Russia

G. Lebedeva

Sechenov First Moscow State Medical University (Sechenov University)

Department of Ear, Nose and Throat Diseases, N.V. Sklifosovsky Institute of Clinical Medicine Moscow, Russia

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