USE OF MOLECULAR BIOLOGICAL TECHNIQUES IN THE ETIOLOGICAL DIAGNOSIS OF OPPORTUNISTIC INFECTIONS OF THE CENTRAL NERVOUS SYSTEM IN HIV-INFECTED PATIENTS


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Аннотация

Objective. To evaluate the efficiency of molecular biological techniques (polymerase chain reaction (PCR)) in the etiological diagnosis of opportunistic infections of the central nervous system (CNS) in patients with HIV infection. Subjects and methods. Ninety-six patients with HIV infection and clinical signs of CNS lesions were examined in the period January 2009 to June 2010. The examination involved a follow-up clinical study, neurological examination, and laboratory tests of peripheral blood and cerebrospinal fluid (SCF). The results of a postmortem study were taken into consideration in case of a fatal outcome. Results. The DNA detection rate for pathogens of HIV-associated CNS infections was found to be related to the degree of immunodeficiency. PCR versus traditionally used laboratory tests was shown to be highly specific in the differential diagnosis of opportunistic CNS infections. Conclusion. The concurrent study of peripheral blood and SCF increases the efficiency of the etiological diagnosis of CNS lesions in HIV-infected patients.

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Авторлар туралы

A. SAFONOVA

Central Research Institute of Epidemiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

Email: anna.safonova@pcr.ms

B. DOMONOVA

Central Research Institute of Epidemiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

T. SKACHKOVA

Central Research Institute of Epidemiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

T. ROMANYUK

Central Research Institute of Epidemiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

O. SHIPULINA

Central Research Institute of Epidemiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

D. KIREYEV

Central Research Institute of Epidemiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

Yu. VENGEROV

Moscow State University of Medicine and Dentistry, Ministry of Health and Social Development of Russia

E. SEREBRYAKOV

Moscow State University of Medicine and Dentistry, Ministry of Health and Social Development of Russia

E. IVANNIKOV

Infectious Diseases Hospital Two, Moscow Healthcare Department

N. MARTYNOVA

Infectious Diseases Hospital Two, Moscow Healthcare Department

G. SHIPULIN

Central Research Institute of Epidemiology, Russian Inspectorate for the Protection of Consumer Rights and Human Welfare

Әдебиет тізімі

  1. Методические рекомендации по вопросам профилактики и лечения вторичных заболеваний у взрослых и подростков, больных ВИЧ-инфекцией (утв. Минздравсоцразвития России 29.12.2006 № 7128-РХ) М., 2006. - 24 с.
  2. Хоффман К., Рокштро Ю.К. Лечение ВИЧ-инфекции 2009. М.: Р. Валент, 2010. — 648 с.
  3. Перегудова А.Б., Шахгильдян В.И., Цветкова О.О. и др. Структура поражения центральной нервной системы у больных ВИЧ-инфекцией специализированного отделения инфекционной больницы. Тер. арх. 2010; 11: 22—27.
  4. Лечение и помощь при ВИЧ/СПИДе. Клинические протоколы для Европейского региона ВОЗ. Копенгаген: ВОЗ, 2007. - 552 с.
  5. Saag M.S., Graybill R.J., Larsen R.A. et al. Practice Guidelines for the Management of Cryptococcal Disease. Clin. Infect. Dis. 2000; 30: 710-718.
  6. Организация работы лабораторий, использующих методы амплификации нуклеиновых кислот при работе с материалом, содержащим микроорганизмы I-IV групп патогенности. Методические указания МУ 1.3.2569-09. М., 2010. - 31 с.
  7. Либман Г., Макадон Х.Д. ВИЧ-инфекция / Под ред. А.И. Мазуса, Т.П. Бессараба: Пер. с англ. М.: ГЭОТАР-Медиа, 2012. - 560 с.
  8. Centers for Disease Control. Investigations of persons treated by HIV-infected health-care workers - United States. MMWR Morb Mortal Wkly Rep. 1993; 42: 329-331.
  9. NCCLS. Clinical Use and Interpretation of Serologic Tests for Toxoplasma gondii; Approved Guideline. NCCLS document M36-A [ISBN 1-56238-523-2]. NCCLS, 940 West Valley Road, Suite 1400, Wayne, PA 19087-1898 USA, 2004.
  10. Шахгильдян В.И. Цитомегаловирусная инфекции: Инфекционные болезни: национальное руководство / Под ред. Н.Д. Ющука, Ю.Я. Венгерова. М.: ГЭОТАР-Медиа, 2009; 784-796.
  11. Nokta M.A., Holland F., De Gruttola V. et al. Cytomegalovirus polymerase chain reaction profiles in individuals with advanced HIV infection: relationship to CMV disease. J. Infect. Dis. 2002; 185: 1717-1722.
  12. Шахгильдян В.И., Шипулина О.Ю., Каражас Н.В. и др. Лабораторная диагностика цитомегаловирусной инфекции у ВИЧ-инфицированных пациентов. Эпидемиол. и инфекц. бол. 2011; 1: 36—40.

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