DEVELOPMENT OF A DIAGNOSTIC REAL-TIME RT-PCR SYSTEM FOR THE DETECTION OF RNA FROM EBOLA ZAIRE VIRUS


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Abstract

In February 2014, an Ebola virus fever outbreak occurred in the Republic of Guinea and then spread to a number of neighboring countries with a total population of about 25 million people. To prevent the spread of this menacing disease to the Russian Federation, a diagnostic real-time RT-PCR system was developed to detect RNA from Ebola Zaire virus in biological samples. The analytical sensitivity of the system was 1-2 x 10 3 GE/ml. Its high specificity was shown using a representative genetic viral and bacterial specimens and samples from clinically healthy individuals.

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About the authors

Vladimir G. DEDKOV

Central Research Institute for Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare; Research Institute of Occupational Medicine, Russian Academy of Sciences

Email: vgdedkov@mail.ru
Moscow

M. V SAFONOVA

Central Research Institute for Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: marina-iris@mail.ru
Moscow

A. A DEVYATKIN

Central Research Institute for Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare; Research Institute of Occupational Medicine, Russian Academy of Sciences

Email: andreideviatkin@gmail.com
Moscow

A. S DOLGOVA

Central Research Institute for Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: annadolgova@inbox.ru
Moscow

O. V PYANKOV

State Vektor Virology and Biotechnology Research Center

Email: pyankov@vector.nsc.ru
Novosibirsk

A. A SERGEEV

State Vektor Virology and Biotechnology Research Center

Email: artsergeev@mail.ru
Novosibirsk

A. P AGAFONOV

State Vektor Virology and Biotechnology Research Center

Email: gafonov@vector.nsc.ru
Novosibirsk

V. V MALEEV

Central Research Institute for Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: maleyev@pcr.ru
Moscow

G. A SHIPULIN

Central Research Institute for Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Welfare

Email: german@pcr.ru
Moscow

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