MOLECULAR GENETIC ANALYSIS OF HIV-1 AMONG PRISONERS ON ANTIRETROVIRAL THERAPY IN THE PENITENTIARY FACILITIES OF THE SVERDLOVSK REGION


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Abstract

Objective. To carry out a molecular genetic analysis of HIV-1 among prisoners receiving antiretroviral therapy (ART) in the penitentiary facilities. Materials and methods. Viral load was determined in 89 prisoners; out of them 32 individuals with a viral load of >1000 copies/ml were examined for HIV-1 resistance to reverse transcriptase inhibitors and protease inhibitors by a genotypic method. A survey method was used to estimate the degree of compliance with antiretroviral therapy in 138 prisoners. Results. 20 (62.5%) of the 32 examined HIV-1 nucleotide sequences were interpreted as susceptible to 19 antiretroviral drugs. None of the 32 sequences contained mutations of high-level resistance to the prescribed drugs. Genotyping revealed that subtype AFSU was predominant; five genetic variants were CRF03 AB and two variants were CRF63 02A1. Conclusion. The high or multiple resistance of HIV-1 to reverse transcriptase inhibitors and protease inhibitors is not responsible for the virologic failure of ART in prisoners.

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About the authors

Aleksandr V. Alimov

Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: virus@eniivi.ru
Cand. Med. Sci.; Director, Yekaterinburg Research Institute of Viral Infections

Aleksandr V. Ustyuzhanin

Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: ust103@yandex.ru
Head, Laboratory, Urals Federal District Center for AIDS Prevention and Control

Alexey V. Novoselov

Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: ufoaids@mail.ru
Cand. Med. Sci., Head, Urals Federal District Center for AIDS Prevention and Control

Natalia S. Demchuk

Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: demchuk.natalia.s@yandex.ru
Biologist, Laboratory, Urals Federal District Center for AIDS Prevention and Control

Lyubov B. Sannikova

Yekaterinburg Research Institute of Viral Infections, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: Sannickova.lub@yandex.ru
Biologist, Laboratory, Urals Federal District Center for AIDS Prevention and Control

Olga Yu. Kadyrova

Medical Sanitary Unit № 66, Federal Penitentiary Service

Email: inf_msch66@mail.ru
Lieutenant Colonel, Internal Military Service, Deputy Head for Organizational, Methodological, and Clinical Expert Work

Aleksandr P. Pankov

Medical Sanitary Unit № 66, Federal Penitentiary Service

Email: inf_msch66@mail.ru
Colonel, Internal Military Service; Head

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