Markers for systemic inflammation in HIV-infected patients with different blood HIV RNA levels: diagnostic significance and comparative analysis


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Objective. To determine the diagnostic significance of markers for systemic inf lammation in HIV- infected patients according to the level of HIV replication. Materials and methods. The investigation enrolled 224 HIV-infected patients who were divided into 3 main groups according to the level of HIV replication. The serum content of lipopolysaccharide-binding protein (IBP), procalcitonin, and cytokines (TNF-α, II-lß, II-6, II-8,11-10, INF-γ, and INF-a) was estimated by solid-phase enzyme immunoassay. The plasma concentration of HIV RNA was determined by real-time PCR. Results. The mean concentrations of IBP and cytokines were significantly higher in all the patients than in the healthy individuals. There was a moderate correlation between the blood levels of HIV viral load and IBP in the groups of patients with a definable HIV replication level. The HIV-infected patients with HIV viremia were observed to have signs of more active systemic inflammation accompanied by the enhanced production of anti-endotoxin protein and cytokines (TNF-α, 11-10, INF-α, and INF-γ) compared with the patients with an undetectable level of HIV viral load. Conclusion. Antiretroviral therapy can substantially reduce the severity of systemic inflammation in patients with HIV infection, but there is no normalization in anti-endotoxin protein concentrations and cytokine status indicators even during effective treatment.

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Anna Matuzkova

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: matuzkova@yandex.ru
Infectiologist, Polyclinic Department, Southern District Center for AIDS Prevention and Control

Natalia Pshenichnaya

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being; Rostov State Medical University, Ministry of Health of Russia

Email: natalia-pshenichnaya@yandex.ru
MD, Head, Infectious Diseases Department with Course of Childhood Infectious Diseases, Faculty for Advanced Training and Professional Retraining of Specialists; Infectiologist, Infectious Diseases and Parasitology Clinic

Aleksandr Suladze

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: hivrost@mail.ru
Can. Med. Sсi., Head, Southern District Center for AIDS Prevention and Control

Ludmila Dosyagaeva

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: hivrost@mail.ru
Virologist, Clinical and Diagnostic Laboratory, Southern District Center for AIDS Prevention and Control

Tatiana Tverdokhlebova

Rostov Research Institute of Microbiology and Parasitology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: niimicrodouble@yandex.ru
MD, Director

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