The efficiency of molecular genetic methods for the diagnosis of infections caused by S. pneumoniae и H. influenzae

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Objective. To develop and validate reagent kits for the quantitative determination of S. pneumoniae and H. influenzae DNA by real-time polymerase chain reaction (RT-PCR). To evaluate its efficiency versus that of other laboratory diagnostic methods.

Subjects and methods. The authors studied the isolates of S. pneumoniae (n = 181) and H. influenzae (n = 101) and the biological materials from patients with severe community-acquired pneumonia (CAP) in 60 adults and from those with moderate CAP in 66 children. They compared the results of RT-PCR, a bacteriological method, and a rapid test for S. pneumoniae antigen.

Results. In the structure of pathogens of severe CAP, there was a preponderance of S. pneumoniae (63%), H. influenzae were found in 32% of patients. S. pneumoniae was detected by RT-PCR 2-3 times more frequently than by other diagnostic methods. RT-PCR revealed H. influenzae DNA in the sputum of 19 patients; the culture was isolated only in 1 patient. The etiology of CAP in children was established in 91% of cases; of which, the viral etiology was found in 18%; S. pneumoniae was detected in 23%.

Conclusion. RT-PCR was shown to be highly effective in diagnosing infections caused by S. pneumoniae and H. influenzae. When CAP was diagnosed by RT-PCR, the material taken from the lower respiratory tract was more informative than that from the upper one.

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作者简介

Maria Elkina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection, and Human Well-Being

编辑信件的主要联系方式.
Email: melkina@cmd.su
ORCID iD: 0000-0003-4769-6781

Junior Researcher, Laboratory for the Molecular Diagnosis and Epidemiology of Respiratory Tract Infections

俄罗斯联邦, Moscow

Svetlana Yatsyshina

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection, and Human Well-Being

Email: svetlana.yatsyshina@pcr.ms
ORCID iD: 0000-0003-4737-941X

Cand. Biol. Sci.; Head, Laboratory for the Molecular Diagnosis and Epidemiology of Respiratory Tract Infections

俄罗斯联邦, Moscow

Tatiana Tronza

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection, and Human Well-Being

Email: tronza@cmd.su
ORCID iD: 0000-0002-0606-0747

Head in the Area of Laboratory Studies, Laboratory for Clinical Microbiology and Human Microbial Ecology

俄罗斯联邦, Moscow

Svetlana Ratchina

I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia

Email: Svetlana.Ratchina@antibiotic.ru
ORCID iD: 0000-0002-3329-7846

MD; Head, Hospital Therapy Department Two

俄罗斯联邦, Moscow

Tatiana Spichak

I.M. Sechenov First Moscow State Medical University (Sechenov University), Ministry of Health of Russia

Email: tv.spichak@mail.ru

MD; Professor, Department of Pediatrics and Public Health, Institute for Medical Personnel Training, National Medical Research Center of Children’s Health, Ministry of Health of Russia

俄罗斯联邦, Moscow

Vasily Akimkin

Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection, and Human Well-Being

Email: akimkin@pcr.ms
ORCID iD: 0000-0003-4228-9044

Academician of the Russian Academy of Sciences, MD, Professor, Director

俄罗斯联邦, Moscow

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2. Fig. 1. The etiological pattern of CAP in children

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3. 2. Comparison S. pneumoniae and H. influenzae DNA concentrations found in the sputum/aspirates and smears (based on the results of 44 patients)

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