Formation of specific t-cell and humoral immunity in unvaccinated and vaccinated children with pertussis

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Abstract

Objective. Evaluation of the relationship between the formation of antigen (AG) – specific T-cell and humoral immunity and its cytokine regulation in the dynamics of pertussis in children.

Materials and methods. 100 children with pertussis were examined at 2–5 weeks of disease and during the period of convalescence. The balance of IFN-γ and IL-4 cytokines in the blood was compared with the dynamics of the specific immune response to B. pertussis antigens. The humoral response was assessed by enzyme immunoassay using Ridascreen test systems, and the T-cell response was assessed by the proliferation of lymphocytes isolated from the blood of patients after incubation on plates from the same test systems.

Results. In 69–92% of unvaccinated children, antigen-specific T-cells are detected from the 2nd to 12th week from the onset of the disease with a temporary sharp decrease in indicators at the 5–6th week. In vaccinated children with pertussis, the immune response develops as a secondary one, but memory T cells are found in no more than 67% of patients.

Conclusion. The nature of cytokine regulation of antibody formation in the dynamics of pertussis is reflected in the process of formation of memory T-cells and depends on the vaccine history.

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About the authors

Irina M. Fedorova

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Author for correspondence.
Email: vestnik-07@mail.ru
ORCID iD: 0000-0002-0335-2752

Cand. Med. Sci., Leading Researcher, Department of Immunology

Russian Federation, Moscow

Olga P. Popova

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being; Russian Medical Academy of Continuous Professional Education, Ministry of Health of Russia

Email: doctorpopova@yandex.ru
ORCID iD: 0000-0002-1772-5978

MD, Professor, Department of Children’s Infectious Diseases, Russian Medical Academy of Continuous Professional Education, Ministry of Health of Russia; Leading Researcher, Department of Immunology, G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Russian Federation, Moscow; Moscow

Maria S. Blyakher

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: maria.s.b@bk.ru
ORCID iD: 0000-0003-3480-6873

MD, Professor, Head, Laboratory of the Immunology Department

Russian Federation, Moscow

Svetlana I. Koteleva

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: felileo@yandex.ru
ORCID iD: 0000-0003-1878-2234

Cand. Med. Sci., Leading Researcher, Department of Immunology

Russian Federation, Moscow

Tatyana A. Skirda

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: t.skirda@mail.ru
ORCID iD: 0000-0003-4140-1014

Cand. Med. Sci., Leading Researcher, Laboratory of Coccal Infections of the Epidemiological Department

Russian Federation, Moscow

Ivan V. Kapustin

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: vano-kapusta@mail.ru
ORCID iD: 0000-0001-6191-260X

Cand. Med. Sci., Leading Researcher, Laboratory of Coccal Infections of the Epidemiological Department

Russian Federation, Moscow

Zarema K. Ramazanova

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: rzarema@mail.ru
ORCID iD: 0000-0002-9314-3312

Cand. Med. Sci, Leading Researcher, Department of Immunology

Russian Federation, Moscow

Elena A. Tulskaya

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: etul@mail.ru
ORCID iD: 0000-0003-1969-4009

Cand. Med. Sci., Leading Researcher, Laboratory of Coccal Infections of the Epidemiological Department

Russian Federation, Moscow

Svetlana V. Sandalova

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: s.sandalova74@mail.ru
ORCID iD: 0000-0002-4548-9888

Researcher, Department of Immunology

Russian Federation, Moscow

Evgeniy E. Odintsov

G.N. Gabrichevsky Moscow Research Institute of Epidemiology and Microbiology of Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: evgenyodin@yahoo.com
ORCID iD: 0000-0001-5895-2520

Cand. Med. Sci., Senior Researcher, Department of Immunology

Russian Federation, Moscow

Juliya V. Shvetsova

Infectious Clinical Hospital № 1 of the Moscow Healthcare Department

Email: uliashvetsova1983@gmail.com

Infectious Disease Physician, Pediatrician of the 3rd Pediatric Department

Russian Federation, Moscow

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2. Fig. 1. The multidirectional dynamics of T-cell and humoral response formation in vaccinated pertussis patients a - IFN- γ/IL4 ratio; b - proportion of patients whose blood showed memory T-cells, %; c - concentration of IgM antibodies against B. pertussis, U/ml

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3. Fig. 2. Multidirectional dynamics of T-cell and humoral response formation in pertussis in unvaccinated patients a - IFN- γ/IL4 ratio; b - proportion of patients whose blood showed memory T-cells, %; c - concentration of IgM antibodies against B. pertussis, U/ml.

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