Quantitative determination of Epstein–Barr virus DNA in biological materials and EBV-related lung damage in HIV-infected patients
- Authors: Shakhgildyan V.I.1, Yadrikhinskaya M.S.2, Domonova E.A.1, Orlovsky A.A.3, Kanestri V.G.1, Yarovaya E.B.3
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Affiliations:
- Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
- Moscow City Center for AIDS Prevention and Control
- Lomonosov Moscow State University
- Issue: Vol 14, No 4 (2024)
- Pages: 33-40
- Section: Original Investigations
- URL: https://journals.eco-vector.com/2226-6976/article/view/646571
- DOI: https://doi.org/10.18565/epidem.2024.14.4.33-40
- ID: 646571
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Abstract
Objective. Evaluation of the frequency of detection of Epstein–Barr virus (EBV) DNA and its quantitative assessment in biological material samples using real-time PCR; description of the clinical and radiological picture of pulmonary pathology with high concentrations of EBV DNA in respiratory substrates and establishment of the relationship between the concentration of EBV DNA in biological materials and EBV-related lung damage in HIV-infected patients.
Materials and methods. The article presents the results of clinical follow-up and analysis of case histories of 5485 hospitalized HIV-infected patients, 1954 of whom had lung damage. Samples of whole blood (510), sputum (35), bronchoalveolar lavage fluid (BALF) (1026), pleural fluid (PF) (38), bronchial biopsy (BB) (133), lymph nodes (LN) (57) were examined by the RT-PCR method with the determination of EBV DNA, M. tuberculosis, Pn. jirovecii, Candida fungi, cytomegalovirus (CMV), human herpes virus 6a/b in qualitative and quantitative formats. The search for associations between the number of EBV DNA copies in biomaterials and EBV-related organ damage (in particular, lungs) was performed using ROC analysis.
Results. The frequency of EBV DNA detection in biomaterials exceeded 55%, the sensitivity was 84.6–100% with a specificity of 5.3–35.4%. Diagnostically significant concentrations of EBV DNA in blood leukocytes, EBV DNA concentrations in BALF, BB, PZ, LN samples with 95% and 99% probability confirming the EBV nature of pulmonary pathology, as well as low quantitative values of EBV DNA in biosamples with 95–99% probability excluding the EBV etiology of lung damage in patients with HIV infection were determined. EBV-related organ damage was detected in 379 patients. Clinical manifestations and radiographic data of 172 patients with EBV-related pulmonary pathology are described.
Conclusion. Quantitative determination of EBV DNA in biomaterials is of crucial importance for confirming the EBV nature of lung damage. Along with the «classical» pathogens of secondary diseases in HIV-infected patients, EBV can be the cause of severe organ pathology.
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About the authors
Vasily I. Shakhgildyan
Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Author for correspondence.
Email: vishakh@yandex.ru
ORCID iD: 0000-0002-8686-0487
MD, Senior Researcher, Department of Epidemiology and Prevention of AIDS
Russian Federation, MoscowMarina S. Yadrikhinskaya
Moscow City Center for AIDS Prevention and Control
Email: rusalo4ka_88@mail.ru
ORCID iD: 0000-0002-9649-8522
Resident Physician
Russian Federation, MoscowElvira A. Domonova
Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: elvira.domonova@pcr.ms
ORCID iD: 0000-0001-8262-3938
PhD, Head, Opportunistic and Papillomavirus Infections Research Group
Russian Federation, MoscowAleksey A. Orlovsky
Lomonosov Moscow State University
Email: leha-orl@yandex.by
ORCID iD: 0000-0002-0794-4683
Laboratory Researcher, Cardiovascular Disease Mortality Reduction Laboratory, National Medical Research Center of Cardiology, Ministry of Health of Russia
Russian Federation, MoscowVeronika G. Kanestri
Central Research Institute of Epidemiology, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being
Email: kanestri@yandex.ru
ORCID iD: 0000-0002-2234-7094
MD, Senior Researcher, Department of Epidemiology and Prevention of AIDS
Russian Federation, MoscowElena B. Yarovaya
Lomonosov Moscow State University
Email: yarovaya@mech.math.msu.su
ORCID iD: 0000-0002-6615-4315
Ph.D. Phys.-Math., professor, Probability Theory Department, Faculty of Mechanics and Mathematics
Russian Federation, MoscowReferences
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