Characteristics of Brucella melitensis strains isolated in different regions of the Russian Federation

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Objective. Evaluation of the phenotypic and molecular genetic properties of B. melitensis strains isolated in brucellosis-affected territories of the Russian Federation from 1949 to 2019.

Materials and methods. We used 74 strains of the causative agent of brucellosis from the collection of the Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being,and 3 reference strains (63/9, 16M and 706) from the MLVA database http://microbesgenotyping.i2bc.paris-saclay.fr/databases.

Results. The studied strains of B. melitensis were distinguished by significant diversity in 14 VNTR loci. It was revealed that the strains of B. melitensis were divided into two large clusters. One cluster is formed by strains circulating in the Asian part of Russia, and the second cluster is represented by strains from the European part of Russia - the North Caucasian Federal District (NCFD) and the Southern Federal District (SFD).

Conclusion. The obtained data are used for continuous molecular genetic monitoring of the causative agent of brucellosis in the territories of Russia unfavorable for brucellosis, including as an effective tool for retrospective epidemiological analysis, which will allow to qualitatively improve the epidemiological surveillance of this infection. The use of complex data of both phenotypic and genetic nature allows to expand the possibilities of differentiation of strains during the investigation of brucellosis outbreaks in the future.

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作者简介

Natalia Serdyuk

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

编辑信件的主要联系方式.
Email: nata.serdyuk.83@mail.ru
ORCID iD: 0000-0001-5350-0111

Cand. Biol. Sci., Senior Researcher, Laboratory of Collection of Pathogenic Microorganisms

俄罗斯联邦, Stavropol

Elena Zhilchenko

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-7180-1292

Cand. Biol. Sci., Head, Laboratory of Collection of Pathogenic Microorganisms

俄罗斯联邦, Stavropol

Irina Kuznetsova

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-9513-0761

Cand. Med. Sci., Senior Researcher, Biochemistry Laboratory

俄罗斯联邦, Stavropol

Nina Zharinova

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-6718-4877

Cand. Biol. Sci., Senior Researcher, Pathogenic Microorganisms Collection Laboratory

俄罗斯联邦, Stavropol

Dmitry Kovalev

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0000-0002-9366-5647

Cand. Chem. Sci., Head., Biochemistry Laboratory

俄罗斯联邦, Stavropol

Nikolay Shapakov

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0000-0001-9152-4026

Junior Researcher, Biochemistry Laboratory

俄罗斯联邦, Stavropol

Anna Khachaturova

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0000-0002-7459-7204

Researcher, Brucellosis Laboratory

俄罗斯联邦, Stavropol

Olga Belozerova

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0009-0002-5570-557X

Junior Researcher, Laboratory of Collection of Pathogenic Microorganisms

俄罗斯联邦, Stavropol

Manya Karapetyan

Stavropol Anti-Plague Institute, Russian Federal Service for Supervision of Consumer Rights Protection and Human Well-Being

Email: stavnipchi@mail.ru
ORCID iD: 0009-0005-3006-5504

Junior Researcher, Laboratory of Collection of Pathogenic Microorganisms

俄罗斯联邦, Stavropol

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2. Figure. Phylogenetic tree constructed based on MLVA typing at 14 VNTR loci of B. melitensis strains isolated from 1949 to 2019 in Russia

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