Determination of TA- Toxin by Gas Chromatography after Derivatization with Trifluoroacetic Anhydride
- Authors: Filenko I.A.1
-
Affiliations:
- ФГБУН Всероссийский институт научной и технической информации Российской академии наук (ВИНИТИ РАН)
- Issue: Vol 13, No 3 (2023)
- Pages: 216-219
- Section: Аналитика веществ и материалов
- URL: https://journals.eco-vector.com/2227-572X/article/view/629376
- DOI: https://doi.org/10.22184/2227-572X.2023.13.3.216.219
- ID: 629376
Cite item
Abstract
The content of mycotoxins in food products of grain nature is one of the indicators of its safety. The article describes the determination of T-2 toxin in modern laboratory with domestic equipment. A calculation formula is proposed that does not require comparison of peak areas. A wider range of detectable concentrations of T-2 toxin has been established. Statistical processing of the results was carried out and metrological characteristics were calculated.
Full Text
![Restricted Access](https://journals.eco-vector.com/lib/pkp/templates/images/icons/text_lock.png)
About the authors
I. A. Filenko
ФГБУН Всероссийский институт научной и технической информации Российской академии наук (ВИНИТИ РАН)
Author for correspondence.
Email: technologistf@mail.ru
к. т. н.
Russian Federation, МоскваReferences
- Awuchi C. G., Ondari E. N., Ogbonna C. U., Upadhyay A. K., Baran K., Okpala C. O.R., Korzeniowska M., Guiné R. P.F. Mycotoxins affecting animals, foods, humans, and plants: types, occurrence, toxicities, action mechanisms, prevention, and detoxification strategies – a revisit. Foods. 2021. 10(6): 1279.
- Kalantari H., Mousavi M. Review on T-2 toxin. Jundishapur Journal of Natural Pharmaceutical Products. 2010. 5(1): 26–38.
- ГОСТ 34108-2017. Корма, комбикорма, комбикормовое сырье. Определение содержания микотоксинов прямым твердофазным конкурентным иммуноферментным методом. М.: Стандартинформ, 2020. 20 c. GOST 34108–2017. Feed, compound feed, feed raw materials. Determination of mycotoxin content by direct solid-phase competitive enzyme immunoassay. M.: Standartinform Publ., 2020. 20 p.
- Мишина Н. Н., Семенов Э. И., Папуниди К. Х. Применение конъюгата Т-2 токсина с полилизином для обнаружения Т-2 токсина в конкурентном ИФА. Ветеринарный врач. 2017. 4: 33–40. Mishina N. N., Semyonov E. I., Papunidi K. H. Application of a T-2 toxin conjugate with polylysine for the detection of T-2 toxin in a competitive EIA. Veterinarnyj vrach = Veterinarian. 2017. 4: 33–40.
- Adunphatcharaphon S. et al. The evolution of multiplex detection of mycotoxins using immunoassay platform technologies. Journal of Hazardous Materials. 2022. P. 128706.
- Pascale M., Panzarini G., Visconti A. Determination of HT-2 and T-2 toxins in oats and wheat by ultra-performance liquid chromatography with photodiode array detection. Talanta. 2012. 89: 231–236.
- Pascale M., Haidukowski M., Visconti A. Determination of T-2 toxin in cereal grains by liquid chromatography with fluorescence detection after immunoaffinity column clean-up and derivatization with 1-anthroylnitrile. Journal of Chromatography A. 2003. 989(2): 257–264.
- Гогина Н. Н. Содержание T-2 и HT-2 микотоксинов в кормах и их влияние на переваримость питательных веществ у мясных кур: дис. канд. с.-х. наук: 06.02.08 ФНЦ «ВНИТИП» РАН, Сергиев Посад, 2020. 166 с. Gogina N. N. The content of T-2 and HT-2 mycotoxins in feed and their effect on the digestibility of nutrients in meat chickens: dissertation of the Candidate of Agricultural Sciences 06.02.08 FNC VNITIP RAN, Sergiev Posad. 2020. 166 p.
- ГОСТ 34140-2017. Продукты пищевые, корма, продовольственное сырье. Метод определения микотоксинов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием. М.: Стандартинформ, 2020. 20 c. GOST 34140–2017 Food products, feed, food raw materials. Method for the determination of mycotoxins using high-performance liquid chromatography with mass spectrometric detection. M.: Standartinform Publ., 2020. 20 p.
- Krska R. et al. Determination of T-2 and HT-2 toxins in food and feed: An update. World Mycotoxin Journal. 2014. 7(2): 131–142.
- Kotal F. et al. Determination of trichothecenes in cereals. Journal of Chromatography A. 1999. 830(1): 219–225.
- Majerus P., Hain J., Scheer M. T-2 and HT-2 toxin analysis in cereals and cereal products following IAC cleanup and determination via GC-ECD after derivatization. Mycotoxin Research. 2008. 24(1): 24–30.
- Cohen H., Lapointe M. Capillary gas chromatographic determination of T-2 toxin, HT-2 toxin, and diacetoxyscirpenol in cereal grains. Journal of the Association of Official Analytical Chemists. 1984. 67(6): 1105–1107.
- Kong W. et al. Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization. Food chemistry. 2012. 132(1): 574–581.
- МУ 3184-84. Методические указания по обнаружению, идентификации и определению содержания Т-2 токсина в пищевых продуктах и продовольственном сырье. М., 1984. 12 с. MU 3184-84. Guidelines for the detection, identification and determination of the T-2 toxin content in food products and food raw materials. Moscow, 1984. 12 p.
- ГОСТ 33682-2015. Продукты пищевые. Определение Т-2 токсина хроматографическим методом. Минск, 2015. 8 c. GOST 33682-2015. Food products. Determination of T-2 toxin by chromatographic method. Minsk, 2015. 8 p.
- РМГ 61-2010. Государственная система обеспечения единства измерений. Показатели точности, правильности, прецизионности методик количественного химического анализа. Методы оценки. М.: Стандартинформ, 2012. 62 c. RMG 61-2010. State system for ensuring the uniformity of measurements. Accuracy, trueness and precision measures of the procedures for quantitative chemical analysis. Methods of evaluation. M.: Standartinform Publ., 2012. 62 p.
- ГОСТ 34100.3-2017 / ISO / IEC Guide 98-3:2008 Неопределенность измерения. Часть 3. Руководство по выражению неопределенности измерения. М.: Стандартинформ, 2018. 104 c. GOST 34100.3-2017 / ISO / IEC Guide 98-3:2008 Uncertainty of measurement. Part 3. Guide to the expression of uncertainty in measurement. M.: Standartinform Publ., 2018. 104 p.
- Руководство ЕВРАХИМ / СИТАК CG 4. Количественное описание неопределенности в аналитических измерениях. Третье издание. 2012. 165 с. EURACHEM / CITAC Manual CG 4. Quantitative description of uncertainty in analytical measurements. Third edition. 2012. 165 p.
![](/img/style/loading.gif)