Determination of TA- Toxin by Gas Chromatography after Derivatization with Trifluoroacetic Anhydride

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Abstract

The content of mycotoxins in food products of grain nature is one of the indicators of its safety. The article describes the determination of T-2 toxin in modern laboratory with domestic equipment. A calculation formula is proposed that does not require comparison of peak areas. A wider range of detectable concentrations of T-2 toxin has been established. Statistical processing of the results was carried out and metrological characteristics were calculated.

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About the authors

I. A. Filenko

ФГБУН Всероссийский институт научной и технической информации Российской академии наук (ВИНИТИ РАН)

Author for correspondence.
Email: technologistf@mail.ru

к. т. н.

Russian Federation, Москва

References

  1. Awuchi C. G., Ondari E. N., Ogbonna C. U., Upadhyay A. K., Baran K., Okpala C. O.R., Korzeniowska M., Guiné R. P.F. Mycotoxins affecting animals, foods, humans, and plants: types, occurrence, toxicities, action mechanisms, prevention, and detoxification strategies – a revisit. Foods. 2021. 10(6): 1279.
  2. Kalantari H., Mousavi M. Review on T-2 toxin. Jundishapur Journal of Natural Pharmaceutical Products. 2010. 5(1): 26–38.
  3. ГОСТ 34108-2017. Корма, комбикорма, комбикормовое сырье. Определение содержания микотоксинов прямым твердофазным конкурентным иммуноферментным методом. М.: Стандартинформ, 2020. 20 c. GOST 34108–2017. Feed, compound feed, feed raw materials. Determination of mycotoxin content by direct solid-phase competitive enzyme immunoassay. M.: Standartinform Publ., 2020. 20 p.
  4. Мишина Н. Н., Семенов Э. И., Папуниди К. Х. Применение конъюгата Т-2 токсина с полилизином для обнаружения Т-2 токсина в конкурентном ИФА. Ветеринарный врач. 2017. 4: 33–40. Mishina N. N., Semyonov E. I., Papunidi K. H. Application of a T-2 toxin conjugate with polylysine for the detection of T-2 toxin in a competitive EIA. Veterinarnyj vrach = Veterinarian. 2017. 4: 33–40.
  5. Adunphatcharaphon S. et al. The evolution of multiplex detection of mycotoxins using immunoassay platform technologies. Journal of Hazardous Materials. 2022. P. 128706.
  6. Pascale M., Panzarini G., Visconti A. Determination of HT-2 and T-2 toxins in oats and wheat by ultra-performance liquid chromatography with photodiode array detection. Talanta. 2012. 89: 231–236.
  7. Pascale M., Haidukowski M., Visconti A. Determination of T-2 toxin in cereal grains by liquid chromatography with fluorescence detection after immunoaffinity column clean-up and derivatization with 1-anthroylnitrile. Journal of Chromatography A. 2003. 989(2): 257–264.
  8. Гогина Н. Н. Содержание T-2 и HT-2 микотоксинов в кормах и их влияние на переваримость питательных веществ у мясных кур: дис. канд. с.-х. наук: 06.02.08 ФНЦ «ВНИТИП» РАН, Сергиев Посад, 2020. 166 с. Gogina N. N. The content of T-2 and HT-2 mycotoxins in feed and their effect on the digestibility of nutrients in meat chickens: dissertation of the Candidate of Agricultural Sciences 06.02.08 FNC VNITIP RAN, Sergiev Posad. 2020. 166 p.
  9. ГОСТ 34140-2017. Продукты пищевые, корма, продовольственное сырье. Метод определения микотоксинов с помощью высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием. М.: Стандартинформ, 2020. 20 c. GOST 34140–2017 Food products, feed, food raw materials. Method for the determination of mycotoxins using high-performance liquid chromatography with mass spectrometric detection. M.: Standartinform Publ., 2020. 20 p.
  10. Krska R. et al. Determination of T-2 and HT-2 toxins in food and feed: An update. World Mycotoxin Journal. 2014. 7(2): 131–142.
  11. Kotal F. et al. Determination of trichothecenes in cereals. Journal of Chromatography A. 1999. 830(1): 219–225.
  12. Majerus P., Hain J., Scheer M. T-2 and HT-2 toxin analysis in cereals and cereal products following IAC cleanup and determination via GC-ECD after derivatization. Mycotoxin Research. 2008. 24(1): 24–30.
  13. Cohen H., Lapointe M. Capillary gas chromatographic determination of T-2 toxin, HT-2 toxin, and diacetoxyscirpenol in cereal grains. Journal of the Association of Official Analytical Chemists. 1984. 67(6): 1105–1107.
  14. Kong W. et al. Validation of a gas chromatography-electron capture detection of T-2 and HT-2 toxins in Chinese herbal medicines and related products after immunoaffinity column clean-up and pre-column derivatization. Food chemistry. 2012. 132(1): 574–581.
  15. МУ 3184-84. Методические указания по обнаружению, идентификации и определению содержания Т-2 токсина в пищевых продуктах и продовольственном сырье. М., 1984. 12 с. MU 3184-84. Guidelines for the detection, identification and determination of the T-2 toxin content in food products and food raw materials. Moscow, 1984. 12 p.
  16. ГОСТ 33682-2015. Продукты пищевые. Определение Т-2 токсина хроматографическим методом. Минск, 2015. 8 c. GOST 33682-2015. Food products. Determination of T-2 toxin by chromatographic method. Minsk, 2015. 8 p.
  17. РМГ 61-2010. Государственная система обеспечения единства измерений. Показатели точности, правильности, прецизионности методик количественного химического анализа. Методы оценки. М.: Стандартинформ, 2012. 62 c. RMG 61-2010. State system for ensuring the uniformity of measurements. Accuracy, trueness and precision measures of the procedures for quantitative chemical analysis. Methods of evaluation. M.: Standartinform Publ., 2012. 62 p.
  18. ГОСТ 34100.3-2017 / ISO / IEC Guide 98-3:2008 Неопределенность измерения. Часть 3. Руководство по выражению неопределенности измерения. М.: Стандартинформ, 2018. 104 c. GOST 34100.3-2017 / ISO / IEC Guide 98-3:2008 Uncertainty of measurement. Part 3. Guide to the expression of uncertainty in measurement. M.: Standartinform Publ., 2018. 104 p.
  19. Руководство ЕВРАХИМ / СИТАК CG 4. Количественное описание неопределенности в аналитических измерениях. Третье издание. 2012. 165 с. EURACHEM / CITAC Manual CG 4. Quantitative description of uncertainty in analytical measurements. Third edition. 2012. 165 p.

Supplementary files

Supplementary Files
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1. JATS XML
2. Fig.1. T-2-toxin

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3. Fig.2. Chromatographic peak of T-2-toxin (38.93 min)

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Copyright (c) 2024 Filenko I.A.

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