NATURAL POLYPHENOLIC COMPOUNDS IN PHARMACOLOGICAL CORRECTION OF EXTREME CONDITIONS

Abstract

Full Text

Purpose of the work was to study the effect of polyphenolic compounds on the level of physical working capability and psychoemotional state of experimental animals in the conditions of long-term exhaustive extreme stresses. Materials and methods of the study: we have evaluated the working capacity using a model of daily forced swimming “to refusal”, with 20% exertion of body weight of an animal. We used white outbred male mice in our experiment weighed 20-25 g, previously ran-domized by the swimming time and divided into 3 groups by 10 animals. Polyphenolic compounds under study - catechine hydrate (Sigma-Aldrich) and morin hydrate (Sigma-Aldrich) - were injected per os at dose 100 mg/kg 60 minutes before the swimming. Control group of animals received 0.9% solution of so-dium chloride in equvolume. The runs lasted 15 days, and on the 5th, 10th, 15th day (1st, 2nd, 3rd test respectively) physical and psychoemotional status of experimental animals was evaluated using an “open field” test. The results of runs were processed by using a method of variation statistics. We calculated an average value and standard error of an average value. The data obtained was checked for distribution normality with the use of Kolmogorov-Smirnov criteria. In case of normal distribution of data for the comparison of average values we used a par-ametric Student’s t-criterion. In case of normal distribution of the experiment results further statistic processing of the data was carried out by using a Mann-Whitney U-criterion. Results of the study: after 15 days of the runs we established the decrease of working capacity of control mice by 48.1% (p>0.05) concerning the initial value of this group of animals, which was accompanied by the expansion of a psychoneurological deficit (by the end of an experiment the number of crossed zones of the “open field” lowered 1.14 times, the number of stand lowered by 79.5% (p>0.05), grooming duration increased by 26.8% concerning the results of the 1st test). Implementation of the polyphenolic compounds under study conduced the working capacity conservation of experimental animals in the setting of exhaustive stresses. After the implementation of catechine hydrate maximum working capacity was noted on the 14th day of the runs and was higher than the stamina level in a control mice group on the same day by 199.1% (p>0.05) and by 58.7% considering the initial indices of this group. Peak of physical activity after the morin hydrate injection was manifested on the 12th day of swimming with loads and amounted to 494.62±74.904 sec, which was longer duration of swimming than of control animal group on the same day by 196.2% (p>0.05). Analyzing the psychoemotional and physiological status of animals in the “open field” test we established that there were no statistically significant differences among the control group and ani-mals which were injected with catechine hydrate at all stages of the test. While the implementation of morin hydrate conduced the increase (considering the control) of locomotor and vertical exploratory activities in 1.1 times and by 92% respectively, and 3.1 times decrease of grooming duration after the 3rd test in the “open field”. Conclusions: polyphenolic compounds under study - catechine hydrate and morin hy-drate - equally conduce the working capacity preservation, besides, the implementation of morin hydrate conduced the normalization of psychoneurological status of the experimental animals in the settings of extreme stresses.