EFFICACY OF PHARMACOTHERAPY OF SYSTEM AUTOIMMUNE INFLAMMATION PROCESS OF COMBINATIONS OF IBUPROFEN WITH VINBORON ON THE MODEL OF ADJUVANT ARTHRITIS OF RATS

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Analyzing the literature data about the pharmacological activity of modern non-steroidal anti-inflammatory drugs structure (NSAID), it should be noted that despite the unquestionable clinical effectiveness of NSAID use has certain limitations, which can be explained by severe side effects and compli-cations, the biggest number of which is accounted for gastrotoxicity. Modern therapeutic approaches for prevention and treatment of NSAID-induced gastropathy unfortunately rarely aimed at developing a set of preventive measures and prevent the expansion of side effects of this group of drugs. Given the diversity of the pathogenesis of NSAID-induced gastropathy, and based on the views of other researchers, we consider it reasonable to use the easing the side effects of NSAIDs drugs with polytropic pharmacological effects, in particular vinboron for this purpose. Purpose of the work was to give a com-parative assessment of the specific activity of ibuprofen and its combination with vinboron (resynthesized Рhеnicaberаnum at “Borschagovsky HFZ”) in the adjuvant arthritis model in rats. Materials and methods of the study: the investigation was carried out using 28 adult male rats, divided into 4 groups. I group included intact rats (n=7), II group included rats with simulated adjuvant arthritis (AA) without treat-ment (control), III group included rats with AA (n=7), ibuprofen (218 mg/kg), IV group included rats with AA (n=7) treated with ibuprofen / w (218 mg/kg) in combination with vinboron (11 mg/kg). AA was modeled by the subplantar administration of Freund's complete adjuvant in the right hind paw of 0.1 ml per rat. Treatment with AA was carried out from 14 to 28 days by intragastric administration of the drugs under study. Results: the treatment with AA combina-tion of ibuprofen with vinboron led to a more signified anti-inflammatory and analgesic activity of ibuprofen due to the potentiation of the pharmacological effects of both drugs. This was proved with a statistically significant inhibition of the inflammatory response by 35.9% compared to 14 days of the experiment, which is 6% higher than in the ibuprofen alone. It is also found that the increase in pain threshold in the combined use of ibuprofen and vinboron was significantly higher by 23.3% than similar rates in ibuprofen monotherapy. Also a combination of ibuprofen with vinboron, unlike most of ibuprofen caused a normalization of the erythrocyte sedimentation rate (2.53 mm/h) which is compared with virtually intact animals (2.29 mm/h). We noted a distinct reduction of white blood cells (7.56*109/l), increase of the number of red blood cells by 23.3% and he-moglobin level by 10.5% in relation to the control animals, indicating that the elimination of signs of anemia and more distinct weakening of inflammatory reaction in comparison with a group of ibuprofen alone. According to the degree of influence on the dynamics of the biochemical blood indices in the setting of the adjuvant arthritis combined use of ibuprofen vinboronom unlike the ibuprofen monotherapy, with normalization of malondialdehyde, superoxide dismutase, seromucoid, gamma-glutamyl transpeptidase, restoring the level of lipids, including phospholipids did not cause negative changes in the activity alanine ami-notransferase, aspartate aminotransferase, alka-line phosphatase, and blood concentration in urea. Also, the combination of ibuprofen with vinboron contributed the increase in total protein content that can be interpreted as a lack of them, in contrast to ibuprofen alone, the ability to inhibit protein synthesis, disrupt the functional state of the kidneys and liver. The level of al-kaline phosphatase (0.40 µmol /ml/min.) in the setting of the combined therapy was comparable with virtually intact animals, indicating a weakening of the destructive changes in the connective tissue caused by the development of adjuvant arthritis in rats. Conclusions: evaluating the results of the study conducted we can conclude that the combination of ibuprofen with vinboron has a great anti-inflammatory and analgesic action in the setting of adjuvant arthritis in rats, than with the monotherapy with mentioned antiphlogistic. Besides, this combination conduces the augmentation of ibuprofen safety.