DETERMINATION Of AN ANTIOxIDANT ACTIvITY Of A TRANSDERMAL OINTMENT WITH SCHISANDRA CHINENSIS

Abstract

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Purpose of the work was to establish an antioxidant activity of a transdermal ointment with extract of Schisandra chinensis, compared with an ointment which contained Mexidol. Materials and methods of the study: the study was carried out using adult Wistar white rats of both sexes and the same age. The animals were kept in accordance with the rules of good laboratory practice in identical conditions, received a standard diet of vivarium and were involved in an experiment at the same time. To assess the nature of the change in state of the main links of the antioxidant system we reproduced toxic liver damage with carbon tetrachloride. The experiment involved four groups of animals: intact group, the control group, the group of rats that was exposed to transdermal ointment with extracts of lemongrass and groups who receive the comparison drug - ointment with Mexidol. All drugs were applied during 6 days concurrently with the administration of carbon tetrachloride. Then the animals were killed and liver homogenates was determined by the content of malondialdehyde, which is the end product of lipid peroxidation and antiradical activity and antiperoxidant enzymes (catalase and superoxide dismutase) according to standard methods and using spectrophotometry. Results: in the setting of an experimental toxic affection of the liver of animals we noted almost double increase in the level of malondialdehyde compared with an intact group of rats (8.10 ± 0.15 mmol/L compared to 4.80±0.22 mmol/l), and the reduction of catalase activity (10.15±0.10 MAb/l as compared to intact 1.12±17.20 MAb/l) and superoxide dismutase (9.95±0.11 activity units compared with the intact 14.23±1.14 activity units). We have found that the use of a transdermal ointment with the extract of Schisandra chinensis has an impact on the content of malondialdehyde in liver tissues of animals (5.58±016 mmol/l), comparable to the effect of mexidol (5.92±0.12 mmol/L) in the same dosage form. Furthermore, the animals of both experimental groups had increasing activity of a catalase enzyme (12.27±0.12 MAb/l ointment with lemon and 13.05±0.15 MAb/l for a similar formulation with Mexidol) and superoxide dismutase with ointment with lemon grass is only slightly inferior to the transdermal dosage form with Mexiidol (11.28±0.11 activity units and 13.91±0.15 activity units respectively). Conclusion: thus, transdermal ointment with CO2, an extract from Schisandra chinensis exhibits antioxidant activity comparable with the activity of Mexidol making prospective study of the dosage form of Schisandra chinensis vine to correct disadaptation of the organism to physical stress.