INFLUENCE OF THE GLUTAMINIC ACID DERIVATIVE ON THE SURVIVAL CAPABILITY OF LABORATORY ANIMALS IN CONDITIONS OF A TOTAL CEREBRAL ISCHEMIA

Abstract

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Purpose of the work was to estimate the efficacy of a preventive effect of glutamic acid derivative with a laboratory code LHT 1-01 at doses of 1, 10, 50 and 100 mg/kg in conditions of total brain ischemia. Materials and methods of the study: the runs were performed using 72 male Wistar rats weighed 200-220 g. Total cerebral ischemia was simulated using gravitational overloads in cranio-caudal position. To simulate hypergravity we used 2 m diameter centrifuge, designed by the Honored Worker of Science Professor M.D. Gaevoy. Awake rats were placed into special containers, cupboards, attached to the end of the centrifuge arm, and a powerful engine for 10 seconds to create a necessary degree of gravity. The magnitude of the gravitational accelerations were adjusted so that the mortality of animals in the control group would amount to not more than 20% (21g over 12 min). The gradient of rise and fall of the exertions amounted to 0.30-0.33 U/sec. All groups of animals 60 min before modeling pathology were injected with an equivalent volume of fluid (physiological solution for the rats in the control group, the object of study at doses 1, 10, 50 and 100 mg/kg in the experimental groups). An increase in the survival of the rats of the experimental group (%) relative to the control animals was the efficacy criterion of the compound. Results: the control group animals survival capability amounted to 12.5%. Preventive injection of LHT 1-01 increased the survival capability of the rats after the hypergravitation at doses 10, 50 and 100 mg/kg respectively up to 50, 25, and 50%. Conclusions: we have established experimentally that the efficacy of the application of the glutaminic acid derivative under study as an anti-ischemic drug in the conditions of a total cerebral ischemia is conditioned by the dose injected. We should note that doses 10 and 100 mg/kg have an similar signified anti-ischemic effect, increasing the animals survival capability up to 50%.