STUDY FOR ANTIOXIDANT ACTION OF DRY EXTRACT OF SCUTELLARIA BAICALENSIS

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Purpose of the work: study of an antioxidant action of the dry extract of Scutellaria baicalensis in vitro and in vivo. Materials and methods of the study: we used a dry extract of roots of Scutellaria baicalensis (DE SB) with baicaline content 95% and water-soluble form of the dry extract with 2-hydroxypropyl-β-cyclodextrin (DE-2-HP-β-CD). The runs were carried out using 60 male rats weighed 190-200 g. During 14 days experimental animals received DE SB at dose 300 mg/kg perorally, DE-2-HP-β-CD abdominally at dose 15 mg/kg. Animals groups which received an equivolume quantity of water orally (control 1), and abdominally (control 2) and 2-HP-β-CD at dose 15 mg/kg abdominally (control 3) were taken as the control. Intensity of lipids peroxidation (LPO) was evaluated by MDA accumulation and the content of diene conjugates (DC) in homogenates of liver and kidneys. Antioxidant activity of DE SB in vitro was studied on a model scheme of Fe2+ascorbate induced LPO liposomes in comparison with quercetine. Results: we have established that in the kidneys of rats which received DE SB and DE-2-HP-β-CD, Fe2+ascorbate induced LPO intensity was relevantly lower that in the control rats by 36% and 35% respectively. The content of DC in homogenates of kidneys was relevantly lower by 48% in the animals which received DE-2-HP-β-CD in comparison with norm and control. While studying the effect of the DE SB and DE-2-HP-β-CD for an intensity of LPO and antioxidant system of liver in the course injection to healthy animals we established the decrease of Fe2+ascorbate induced LPO intensity in liver comparing with the corresponding control groups by 52% and 37% respectively. The content of DC in homogenates of liver in animals which received DE SB was lowered by 40%, and in the animals which received DE-2-HP-β-CD the level of DC did not differ relevantly from the corresponding control. The decrease of LPO products content can be a consequence of the antioxidant action of flavonoids of baicalin - the principal component of DE SB. Studying for the antioxidant activity in vitro we have revealed that baicalin in its minimum concentration studied (3.1 µmol) lowered the accumulation of peroxide products by 23%, and quercetine at concentration 1 µmol by 21%. Concentration, with which the LPO intensity was almost entirely suppressed, amounted to 100 µmol/l for baicalin and quercetine (88% and 94% respectively). IС50 coefficient was calculated for the comparison of an antioxidant activity (AOA). It amounted to 21.7 µmol/l for baicalin, and 35.8 µmol/l for quercetine. Conclusions: thus, while studying for the effect of a course implementation of DE SB and DE-2-HP-β-CD on the LPO processes we have established that in the animals organism there are effects registered, which give evidence about their antioxidant action in conditions in vivo. It is more likely to be determined with baicalin flavonoid, for which there is rather signified (comparable with quercetine) capability of slowing down the LPO on a model system in vitro was established. Antioxidant activity of baicalin, which is the principal component of DE SB, plays an important role in liver and kidneys protection from the oxidative stress.