METHOD OF CORRECTION OF THE ULCER DEFECT OF GIT TISSUES WITH MAGNESIUM-BEARING COMPOSITION IN STRESS RESISTANT RATS IN THE EXPERIMENT

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Purpose of the work was to determine the influence of magnesium-bearing therapeutic bischofite composition to the area of the ulcer and the content of magnesium in biological media in stress resistant rats with experimental acetate gastric ulcer. Materials and methods of the study: three series of experiments were carried out on 21 Wistar rats. Stress resistant rats were pre-determined using an open field test. In the first control series on 7 rats we simulated acetate ulcer modeling without damaging of the stomach mucous membrane of with acetic acid. In the second and third series (7 rats) acetate ulcer was simulated in prepyloric zone with the method of S. Okabe (2005). The rats of the third series were given with suppositories with Magnesium-bearing therapeutic composition (MBTC) based on bischofite polymineral per rectum during 7 days after modeling ulcers. The animals were taken out of the experiment after 7 days from its start. The content of magnesium (Mg2+) were determined in red cell blood from the subclavian and portal veins (Vs, Vp) by V.S. Kamyshnikova (2004), in the blood plasma - a set of reagents produced by "Lachema" company. Results: modeling of an acetate ulcer in stress resistant animals led to the appearance of the ulcer area of 38.72±4.09 mm2. In the setting of the MBTC application the area of ulcers in stress resistant rats with a forming acetate ulcer decreased by 30.3% (P <0.05) and amounted to 27.00±0.92 mm2. Comparative investigation of Mg2+ content in blood of the stress resistant rats of the control group in relation to the initial state showed its higher content in the blood plasma from Vs by 12.7% (P <0.001) and packed red cells from the same regions of 23.8% ( P <0.05). Experimental modeling of acetate ulcers in stress resistant animals was accompanied by various degrees of reduction of the Mg2+ content in blood in relation to the control animals, but statistically significantly decreased its level in blood of the red cell Vs and Vp by 31.1% (P <0.001) and 21.9 % (P <0.05) respectively. Study of Mg2+ content in stress resistant animals with an acetate ulcer in the setting of magnesium-bearing treatment composition showed that its concentration in blood plasma of the Vs and Vp decreased by 11% (P <0.001), 10.1% (P <0.05) respectively. The level of Mg2+ in packed red cells from these regions increased by 52.1% (P<0.001), and 55.9% (P<0.001) respectively. Conclusions: thus, under the influence of magnesium-bearing treatment composition decreases the area of the ulcer in stress resistant animals with experimental ulcerative defects, which is accompanied by an increase in the concentration of magnesium in the setting of endoglobular reduce of its level in a blood plasma.