INVESTIGATION FOR THE INFLUENCE OF DIFFERENT MAGNESIUM SULFATE DOSES ON THE CONTENT OF CALCIUM AND CHLORIDE IN BLOOD SERUM OF RATS OF BOTH GENDERS

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Introduction: At present, obstipation conditions are frequently encountered. According to some foreign authors chronic constipation is encountered in 1.9-27% of patients aged under 65 [3]. People after 65 years have this condition in 12.5-30% of cases [5]. But of all patients with this kind of disorders only 34% call for medical help [6]. As the result the frequency of uncontrollable, self-treatment with depletive agents increase, which often leads to undesirable side effects [4]. As the result of some depletive agents administration, particularly magnesium sulfate, there can be a high frequency of electrolytic defects [1], which determined the purpose of this investigation. Purpose of the study is to research the impact of different magnesium sulfate doses on the content of calcium and chloride in blood serum of both gender rats. Materials and methods of the study. The experiment was carried out using outbred reproductive Wistar rats of both gender, weighed 220-240 grams. According to the trials protocol there were 3 experimental animal groups formed (6 male and 6 female rats in each) [2]. The first group rats were taken as control. The drug under study “Magnesium sulfate, powder for solution preparation and internal administration” (“Tula pharmaceutical plant” ltd) was injected in 2 dosages. The first dose was close to the maximum therapeutic, considering calculation factor for these animals, and amounted to 1.7 g/kg (minimum dose). The second dose was to reveal possible toxic action of the drug and amounted to 5 g/kg (maximum dose, triple maximum therapeutic dose). Meanwhile the control group of rats was given a clean water in the same volume. The drug under study was administered per os during 7 days. On the 8th day all experimental animals were sacrificed by decapitation under chloral hydrate anesthesia (350 mg/kg) and took a blood sample for further blood serum extraction (centrifuging), and determination of the content of calcium and chloride. Concentration of chlorides (mmol/l) was calculated by using phorometry with DiaSys reagents set. The method’s principle is based on the capability of chlorides to free the same quantity of thiocyanate from mercury thiocyanate (II). Thiocyanate together with ferrum ions forms a red color complex, light adsorption of which is proportional to the chlorides concentration. To determine calcium concentration we used photometric text with DiaSys reagents set. In neutral medium, calcium responds to arsenazo III, forming a blue-colored complex. Color intensity was proportional to the calcium concentration. Magnesium influence is eliminated by adding 8-hydroxiquinoline-5-sulphonic acid. Calcium concentration was evaluated in mmol/l. All determinations were done using system of automatic biochemical analyzer BS- 380 (Mindray). The experiments results were processed by the method of variation statistics. The average value and standard error of average value (M±m). The data obtained were checked on normality of distribution using Kolmogorov-Smirnov’s test. In case of normal distribution of data parametric Student’s t-criteria was used to compare average values. If distribution of results was abnormal, further statistic processing was done using Mann-Whitney U test. StatPlus 2009 program package was used for statistic processing of the results. Results Administration of minimum dose of sulfate to male rats led to significant reduction of chloride level in blood serum, comparing with control group of rats from 105.72±0.52 mmol/l to 103.27±0.88 mmol/l. The calcium content in animals which were given a minimum dose of magnesium sulfate did not differ significantly from the same index in the control group. A week long peroral administration of magnesium sulfate at minimum dose did not led to significant changes of chlorides and calcium content in blood serum of female rats (table 1). After the administration of maximum dose of the drug under study there was a reduction of chloride content of both indexes under study, in male rats as well as female rats. The content of calcium in a group of male rats was equal to the female indexes, and it was significantly lower the indexes of control group of animals (table 1). Conclusions: Administration of magnesium sulfate at maximum therapeutic dose 1.7 g/kg, during 7 days leads to relevant reduction of chlorides content in blood serum of male rats comparing with control group of animals. After administration of triple dose there is reduction of calcium concentration in rats - male by 9%, and female by 4%. The content of chlorides, concerning the control group decreases equally in males and females. Thus, triple therapeutic dose of magnesium sulfate negatively influence the chlorides and calcium concentration in blood serum of both gender rats.

作者简介

A. Voronkov

Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University

Email: a.v.voronkov@pmedpharm.ru
Pyatigorsk, Russian Federation

A. Terekhov

Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University

Email: tau200@yandex.ru
Pyatigorsk, Russian Federation

N. Dyakova

Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University

Email: irochkadyakova@mail.ru
Pyatigorsk, Russian Federation

N. Avramenko

Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University

Pyatigorsk, Russian Federation

D. Pozdnyakov

Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University

Email: pozdniakow.dmitry@yandex.ru
Pyatigorsk, Russian Federation

S. Kuleshova

Pyatigorsk Medical and Pharmaceutical Institute - branch of Volgograd State Medical University

Pyatigorsk, Russian Federation

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