INFLUENCE OF BIOLOGICAL ACTIVE SUBSTANCES OF FILIPENDULA VULGARIS ON THE PHYSICAL PERFORMANCE IN THE EXPERIMENT

详细

全文:

Purpose of the work was to study an adaptogenic activity of water and water-ethanol extract of the aboveground parts of Filipendula vulgaris by its influence on the working performance of animals. Materials and methods of the study: aboveground part of Filipendula vulgaris Moench was the object of the study. Extracts were obtained by the procession of plant raw materials with water and ethanol of different concentrations (40%, 70%, and 95%) on water bath with a return condenser. Boiling down of the extracts up to dry was carried out in vacuum at temperature higher 60 оС. Water extract contains 2.56% of flavonoids in terms of glycosides of quercetine; dry residue amounts to at least 15%. We carried out the investigations using 100 outbred male mice CD-I (I category, according to the certificate) weighed 20-22 g. We used the animals from the Department of Experimental Biological Models at E.D. Goldberg Scientific and Research Institute of Pharmacology and Regenerative Medicine. We studied the influence of physical performance in a forced swimming test with load of 10% of mouse weigh at water temperature 28 ºС. We made this test two times a day with 1 hour break during 5 days. On the 6th day of the runs we evaluated the depression of working capacity, provoked by the water temperature fall to 16 ºС. Extracts were injected into the animals every day during 5 days through the sound into a stomach in water 1 hour before the experiment manipulations. The animals of control groups were given with an equal quantity of water. Experimental data were processes statistically with the use of Student’s t-criterion and non-parametric Wilcoxon-Mann-Whitney criterion. Results: in the conditions of the forced swimming in the group of control animals we saw the incremental improvement of performance, which characterized the process of adaptation to physical exertions, and its sharp fall after the water cooldown on the 6th day. After the injection of Filipendula vulgaris Moench on 40% ethanol at doses 10 and 50 mg/kg we saw the increase of swimming duration in comparison with the control. Doses 200 and 1000 mg/kg did not influence on the rate under study in relation to the control group. The extract at dose 50 mg/kg showed the maximum activity even with depression on the 6th day of the runs. While conducting experimental studies of the influence of water and water-ethanol extracts of the plant on physical performance in all animal groups, which were given with the extract beginning with the first day of runs we noted the increase of swimming time in comparison with the control. The most durable and statistically significant rate increase took place on the 1st-4th day of the experiment with the injection of a water extract to the animals (in 1.5-1.9 times), a on the 2nd-4th day in case of use of the extract based on 70% ethanol (in 1.6-2.0 times). After the water cooldown to 16 ºС on the 6th day of the runs significant changes on the side of the rate raise (in 1.6-1.8 times) are manifested with the injection of extracts on water, 70% and 95% ethanol in comparison with the control. Conclusions: this, the extracts of the aboveground part of Filipendula vulgaris Moench exhibit adaptogenic activity. The most signified properties belong to the water extract of the plant at dose 50 mg/kg, which is conditioned by the presence of the fullest sum of substances of phenolic (simple phenols, flavonoids, hydroxycoumarins, phenolcarbonic acids, tannins), triterpenic (acids and saponins), and carbohydrate (including water-soluble polysaccharides) nature and amino acids.