STUDY OF THE COMPONENT COMPOSITION OF POLYPHENOLES OF THE KURIL TEA PLANT ΈPENTAPHYLLOIDES FRUTICOSA L.Ή

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Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150ɍȾК 577.13:582.71: 543.544 ʰʯ˄ˋʫʻʰʫКОʺʿОʻʫʻ˃ʻОʧОˁОˁ˃ʤʦʤʿОЛʰˇʫʻОЛОʦ˃ˀʤʦˏPENTAPHYLLOIDES FRUTICOSAМɚлюɬинɚПɪɚɜлоцкɚяНоɜикоɜПиɫɚɪɟɜɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииɊоɫɫияȻɟлɝоɪоɞПоɛɟɞыКɭɪильɫкийяɜляɟɬɫяпопɭляɪнымɪɚɫɬɟниɟмнɚɪоɞноймɟɞициныКиɬɚяМонɝолииИмɟющиɟɫянɚɭчныɟɫɜɟɞɟнияɫɜиɞɟɬɟльɫɬɜɭюɬɜыɪɚжɟнныɯɚнɬиокɫиɞɚнɬныɯɫɜойɫɬɜɫопɪянɚличиɟмполиɮɟнолоɜɌɚкжɟɭɫɬɚноɜлɟнɪяɞɞɪɭɝиɯɮɚɪмɚколоɝичɟɫкиɯэɮɮɟкɬоɜɚнɬиɛɚкɬɟɪиɚльныйɮɭнɝициɞныйɝипоɝликɟмичɟɫкийпɪоɬиɜоɜоɫпɚлиɬɟльныйпɪоɬиɜоязɜɟнОɞнɚкоɫоɞɟɪжɚниɟполиɮɟнолоɜɫоɫɬɚɜɟɜɚɪьиɪɭɟɬɝɟолоɝичɟɫкойзоныкоɬоɪойпɪоизɪɚɫɬɚлооɬɪɚжɚɟɬɫяɬɟɪɚпɟɜɬичɟɫкойɚкɬиɜноɫɬиПоэɬомɭцɟльюнɚɫɬоящɟɝоиɫɫлɟɞоɜɚнияяɜилоɫьизɭчɟниɟкɚчɟɫɬɜɟнноɝоɫоɫɬɚɜɚполиɮɟнолоɜ P. fruticosa ɫɬɚнɞɚɪɬизɚцииМɚɬɟɪиɚлымɟɬоɞыкɚчɟɫɬɜɟоɛъɟкɬɚиɫɫлɟɞоɜɚнияɜзяɬоɛɪɚзɟц P. fruticosa L., ɫоɛɪɚнныйȺлɬɚйɫкоɝопɪоɜоɞилɫяпомощьюɜыɫокоэɮɮɟкɬиɜнойжиɞкоɫɬнойɯɪомɚɬоɝɪɚɮииоɛɪɚщённоɮɚзномɜɚɪиɚнɬɟɊɟзɭльɬɚɬыоɛɫɭжɞɟɍɫɬɚноɜлɟнɯимичɟɫкийɫоɫɬɚɜкоɬоɪыйɜключɚɟɬ 18 ɫоɟɞинɟнийполиɮɟнольнойпɪиɪоɞыпɪɟɞɫɬɚɜлɟнныйɝликозиɞɚмиɚɝликонɚмиɮлɚɜонолоɜокɫикоɪичнымикиɫлоɬɚмиэллɚɝоɬɚннинɚмиМɟɬоɞомноɪмɚлизɚцииɪɚɫɫчиɬɚнооɬноɫиɬɟльноɟɫоɞɟɪжɚниɟкɚжɞоɝокомпонɟнɬɚɫɭммɟɪɟзɭльɬɚɬɟɭɫɬɚноɜлɟнопɪɟоɛлɚɞɚющимиɝликозиɞыкɜɟɪцɟɬинɚОпɪɟɞɟлёнɫоɫɬɚɜɚɝликоноɜɮлɚɜонолоɜпоɫлɟкиɫлоɬноɝоɝиɞɪолизɚɜключɚющийкɜɟɪцɟɬинкɟмпɮɟɪолɪɚмнɟɬинпɪɟоɛлɚɞɚниɟмпɟɪɜоɝокомпонɟнɬоɜкиɫлоɬноɝоɝиɞɪолизɚокɚзɚлɫяяɜляɟɬɫяɜɟɫкимɞокɚзɚɬɟльɫɬɜомɏɚɪɚкɬɟɪноɞоɫɬɚɬочноɜыɫокоɟɫоɞɟɪжɚниɟɫоɫɬɚɜиɜшɟɟоколо 24% оɛщɟйɫɭммыполиɮɟнолоɜЗɚключɟПолɭчɟнныɟɪɟзɭльɬɚɬыпозɜоляюɬɭɬɜɟɪжɞɚɬьɫɬɚнɞɚɪɬизɚциюиɫɫлɟɞɭɟмоɝооɛъɟкɬɚможнопɪоɜоɞиɬьпɟɪɟɫчёɬɟкɜɟɪцɟɬинкɚкɞоминиɪɭющɟɝокомпонɟнɬɚɫɭммыполиɮɟнолоɜпоɫлɟпɪɟɞɜɚɪиɬɟльноɝоɝиɞɪолизɚКлючɟɜыɟɫлоɜɚкɭɪильɫкийɜыɫокоэɮɮɟкɬиɜнɚяжиɞкоɫɬнɚяɯɪомɚɬоɝɪɚɮияɮлɚɜоноиɞыциɬиɪоɜɚнияМɚлюɬинɚПɪɚɜлоцкɚяНоɜикоɜПиɫɚɪɟɜИЗɍЧȿНИȿКОМПОНȿНɌНОȽОɋОɋɌȺȼȺПОЛИɎȿНОЛОȼɌɊȺȼЫPENTAPHYLLOIDES FRUTICOSAɎɚɪмɚцияɮɚɪмɚколоɝияМɚлюɬинɚПɪɚɜлоцкɚяНоɜикоɜПиɫɚɪɟɜSTUDY OF THE COMPONENT COMPOSITION OF POLYPHENOLES OF THE KURIL TEAPLANT PENTAPHYLLOIDES FRUTICOSAA.Yu. Malyutina, A.V. Pravlotskaya, O.O. Novikov, D.I. Pisarev Belgorod National State Research University of the Russian Federation, 85, Pobeda Str., Belgorod, Russia, 308015 c evidence indicates the presence of the plant expressed antioxidant properties, conjugated with the presence of polyphenols. ammatory and antiulcer. However, the content of polyphenols in the plant varies greatly depending on the geological growing region, which affects its therapeutic activity. Therefore, of this research was to study the For citation:Malyutina A.Yu., Pravlotskaya A.V., STUDY OF THE COMPONENT COMPOSITION OF POLYPHENOLES OF THE KURIL PLANT (PENTAPHYLLOIDES FRUTICOSAPharmacy & Pharmacology.2018;6(2):135-150. DOI: 10.19163/2307-9266-2018-6-2-135-150ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, Botanyкɭɪильɫкийɫɟмɟйɫɬɜɚоɬноɫилиɪоɞɭоɞнɚконɚɫɬоящɟɟɟɝоɫмɚɬɪиɜɚюɬкɚкпɪɟɞɫɬɚɜиɬɟльɪоɞɚ. ЭɬопопɭляɪноɟнɚɪоɞноймɟɞицинɟКиɬɚяɌиɛɟɬɚМонɝолииɋɜоёнɚзɜɚниɟполɭчилɬоɝочɬоɟɝоцɜɟɬкиɜыɫɭшɟнномиɫпользоɜɚликɚкнɚɪоɞыпɪожиɜɚющиɟɪиɬоɪииɍɪɚлɚКɭɪильɫкиɯоɫɬɪоɜоɜ [2, 3]. КиɬɚɟэɬоиɫпользɭюɬзɚɛолɟɜɚнияɯжɟлɭɞочкишɟчноɝоɬɪɚкɬɚкɚкɫɪɟɞɫɬɜоɪɟɝɭлиɪɭющɟɟмɟɬɚɛолизммɟнɫɬɪɭɚцииМонɝольɫкойнɚɪоɞноймɟɞицинɟпɪимɟняɟɬɫякɚккɪоɜооɫɬɚнɚɜлиɜɚющɟɟ [4, 5, 6]. нɚɫɬоящɟɝокɚкɞɪɭɝиɯпɪɟɞɫɬɚɜиɬɟлɟйɪоɞɚɯɚɪɚкɬɟɪɟнɜɚжныɯɮɚɪмɚколоɝичɟɫкиɯɫɜойɫɬɜɬɚкиɯкɚкɚнɬиокɫиɞɚнɬныɟпɪоɬиɜомикɪоɛныɟпɪоɬиɜоɜиɪɭɫныɟɝипоɝликɟмичɟɫкиɟпɪоɬиɜоɜоɫпɚлиɬɟльпɪоɬиɜоопɭɯолɟɜыɟɚнɬиɭльцɟɪоɝɟнныɟ [4, 7, 8, 9, 10, 11, 12]. нɚɭчныɟиɫɫлɟɞоɜɚɫɜиɞɟɬɟльɫɬɜɭюɬчɬоɮɟнольныɟкомпонɟнɬыP. L., пɟɪɜɭюочɟɪɟɞьэллɚɝоɜɚякиɫлоɬɚ (+)-кɚɬɟɯинокɚзыɜɚюɬзнɚчиɬɟльнɭюɬиокɫиɞɚнɬнɭюɫпоɫоɛноɫɬьin vitroпɪоɬɟкɬиɜныйэɮɮɟкɬоɬношɟнииокɫиɞɚционномɫɬɪɟɫɫɟ [13, 14]. ɋɪɚɜниɬɟльноɟɮиɬоɯимичɟɫкоɟизɭчɟниɟɬɪɟɯпокɚзɚлочɬоɜɫɟɯпɟɪɟчиɫлɟнныɯоɛъɟкɬɚɯпɪиɫɭɬɫɬɜɭюɬкɚɬɟɯинкоɮɟйнɚяɮɟɪɭлоɜɚякиɫлоɬыэллɚɝоɜɚякиɫлоɬɚȼɫɟпɟɪɟчиɫлɟнныɟпɪояɜлялиɚнɬиокɫиɞɚнɬныɟпɪоɬиɜомикɪоɛныɟɫɜойɫɬɜɚоɬношɟнииɝɪɚмположиɬɟльныɯɛɚкɬɟɪийɬɚкжɟоɬмɟчɟнознɚчиɬɟльноɟɚнɬиокɫиɞɚнɬнойɚкɬиɜноɫɬиэкɫɬɪɚкɬоɜP. L.комɛинɚцииполиɮɟнолɚмизɟлɟноɝоɫооɬношɟнииɫооɬɜɟɬɫɬɜɟнноɏимичɟɫкиɟɮɚɪмɚколоɝичɟɫкиɟиɫɫлɟɞоɜɚпокɚзɚличɬоɜыɫокɭюɚнɬиокɫиɞɚнɬнɭюɬиɜноɫɬьP. fruticosa оɬɜɟɬɫɬɜɟнныполиɮɟнолынɟɫколькиɯɮлɚɜоноиɞыɜоɞныɟкɜɟɪцɟɬинɚкɟмпɮɟɪолɚɞɭɛильныɟщɟɫɬɜɚ [17]. ОɞнɚкоɫоɞɟɪжɚниɟɮɚɪмɚколоɝичɟɫкизнɚчимыɯкомпонɟнɬоɜɫоɫɬɚɜɟɜɚɪьиɪоɜɚɬьзɚɜиɫимоɫɬиэколоɝоцɟноɬичɟɫкиɯпɪоизɪɚɫɬɚнияЭɬооɬɪɚжɚɟɬɫяколичɟɫɬɜɟɛиолоɝичɟɫкиɚкɬиɜныɯɫоɟɞинɟнийкɚкɫлɟɞɫɬɜиɟɬɟɪɚпɟɜɬичɟɫкойɬиɜноɫɬи [18, 19, 20, 21, 22, 23]. эɬойɫɜязиоцɟнкɚкɚчɟɫɬɜɟнноɝоɫоɫɬɚɜɚколичɟɫɬɜɟнноɟɫоɞɟɪжɚɬɪɚɜɟP. fruticosa яɜляɟɬɫяɚкɬɭɚɛлɟмойɍчиɬыɜɚяɜышɟɫкɚзɚнноɟЦȿЛЬЮнɚɫɬоящɟɝоиɫɫлɟɞоɜɚнияяɜилоɫьизɭчɟниɟкɚчɟɫɬɜɟнноɝоɫоɫɬɚполиɮɟнолоɜɬɪɚɜыP. fruticosa ɫɬɚнɞɚɪɬизɚИзɭчɟниɟɞɚнноɝопɪоɜоɞиɬɫяɪɚмкɚɯноɜоɝонɚɭчноɝонɚпɪɚɜлɟниямɚцɟɜɬичɟɫкийМȺɌȿɊИȺЛЫМȿɌОȾЫкɚчɟɫɬɜɟоɛъɟкɬɚиɫɫлɟɞоɜɚнияɬɪɚɜыP. fruticosa L.ɬɟɪɪиɬоɪииȺлɬɚйɫкоɝоПолиɮɟнольныйкомплɟкɫɬɪɚɜыP. fruticosa изɜлɟкɚлипомощьюɫпиɪɬɚэɬилоɜоɝо 70%-концɟнɬɪɚцииɫооɬношɟнии 1:50 экɫɬɪɚɝɟнɬɭПолɭчɟнноɟизɜлɟчɟниɟɯɪомɚɬоɝɪɚɮиɪоɜɚлипɪɟɞɫɬɚɜлɟнныɯнижɟȺнɚлизпɪоɜоɞилɫяпомощьюɜыɫокоэɮɮɟкɬиɜжиɞкоɫɬнойɯɪомɚɬоɝɪɚɮииномɜɚɪиɚнɬɟɏɪомɚɬоɝɪɚɮиɪоɜɚлиɯɪомɚɬоɝɪɚɮɟɮиɪмыAgilent Technologies 1200 Inɏɪомɚɬоɝɪɚɮичɟɫкɚяколонкɚ ? Ascentisexpress кɚчɟɫɬɜɟиɫпользоɜɚныɜоɞɚкɜɚлиɮикɚции HPLC ɫпиɪɬȽОɋɌкиɫлоɬныммоɞиɮикɚɬоɪомɫлɭжилɚкиɫлоɬɚɊɚɫчɟɬɬɟоɪɟɬичɟɫкиɯɬɚɪɟлокɪɚзɞɟляɫпоɫоɛноɫɬиɯɪомɚɬоɝɪɚɮичɟɫкойкоэɮɮициɟнɬɪɚзɞɟлɟнияɚɫиммɟɬɪиюмɚɬоɝɪɚɮичɟɫкоɝопикɚ ? коэɮɮициɟнɬɚɫиммɟɬɪииɪɚɫɫчиɬыɜɚлиɫоɝлɚɫноȿɜɪопɟйɫкойɎɚɪмɚкопɟɟȺɞɟкɜɚɬнымизнɚчɟниямиɬɟоɪɟɬичɟɫкиɯзнɚчɟнийпɪиняɬо 5000, коэɮɮициɟнɬɚɛолɟɟ ? 1,5, коэɮɮициɟнɬɚɚɫиммɟɬɪииqualitative composition of polyphenols in P. fruticosa L. for further standardization of its raw materials. As a research object, a sample of P. fruticosa L. herb collected in the Altai Territory was taken. The analysis was carried out using high-performance liquid chromatography in the reversed-phase version. Results and nature, represented by glycosides and aglycons avonols, proanthocyanidins, oxycinnamic acids, ellagatannins. By the method of internal normalization, the relative content of each component in the sum is has been calculated. As a result, glycosides of quercetin have been found to prevail. After acid hydrolysis the composition of aglycon avonols was determined. It included quercetin, kaempferol and ramnetin, with predominance of quercetin. One of the components of acid hydrolysis was cyanidine, which is a strong proof of the presence of proanthocyanidins in the plant. A fairly high content of proanthocyanidins amounting to about 24% of the total amount of polyphenols is typical of it. The obtained results make it possible to assert that standardization of the investigated object can be carried out in terms of quercetin as the dominant component of the sum of polyphenols after preliminary hydrolysis.Kuril tea (Potentilla fruticosa L.), high-performance liquid chromatography, prolanthocyanidinsPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150ПолиɮɟнольныйкомплɟкɫɯɪомɚɬоɝɪɚɮиɪоɜɚлиɫлɟɞɭющиɯɫкоɪоɫɬьɬɟмпɟɪɚɬɭɪɚɬɟɪмоɫɬɚɬɚколонкиоɛъёмȽɪɚɞиɟнɬныйэлюиɪоɜɚнияпɪоɜоɞилиɭкɚзɚнныɯɬɚɛлицɟɌɚɛлицɚɍɫлоɜияэлюиɪоɜɚнияполиɮɟнолоɜ P. fruticosus LминȺ09010108020207030305050 401090Ɋɟɝиɫɬɪɚциюкомпонɟнɬоɜпɪоɜоɞилиɫлɟɞɭɜолнɮлɚɜонолы ? 355 ɝиɞɪокɫикоɪичныɟкиɫлоɬы ? 310, 325 пɪоɚнɬоциɚниɞиныэллɚɝоɬɚнниныКомпонɟнɬныйɫоɫɬɚɜиɞɟнɬиɮициɪоɜɚлиɫооɬɜɟɬɫɬɜиюɭɞɟɪжиɜɚнияɚнɚлиɬоɜɫɬɚнɞɚɪɬнымиɬɚкжɟɪɟзɭльɬɚɬɚмɞиоɞмɚɬɪичноɝоɞɟɬɟкɬиɪоɜɚнияОɬноɫиɬɟльноɟɫоɞɟɪжɚниɟоɬɞɟльныɯкомпонɟнɬоɜопɪɟɞɟлялимɟɬоɞомɜнɭɬɪɟннɟйноɪмɚлизɚɪɚɫчёɬɜɟлиɮоɪмɭлɟ:ɝɞɟ Si ? ɫɪɟɞнɟɟзнɚчɟниɟпикɚкомпонɟнɬɚɯɪомɚɬоɝɪɚммɚɯɫɭммыɫɪɟɞнɟɟзнɚчɟниɟɫɭммыɜɫɟɯкоɜɯɪомɚɬоɝɪɚммɚɯоɛъɟкɬиɜнойɫоɞɟɪжɚнияɚɝликоноɜɮлɚɜоноиɞоɜпɪоɜɟɞёнкиɫлоɬныйɝиɞɪолизлиɮɟнолоɜɬɪɚɜыP. fruticosa Ƚиɞɪолизпɪоɜоɞилиɪɚɫɬɜоɪомкиɫлоɬыɯлоɪиɫɬоɜоɞоɪоɞнойɬɟчɟ 60 ПолɭчɟнныйɝиɞɪолизɚɬɮильɬɪоɜɚлиɯɪомɚɬоɝɪɚɮиɪоɜɚлиɊȿЗɍЛЬɌȺɌЫОȻɋɍЖȾȿНИȿɏɪомɚɬоɝɪɚммɚɪɚзɞɟлɟнияɫпиɪɬоɜоɝоизɜлɟчɟнияɬɪɚɜыP. fruticosa пɪɟɞɫɬɚɜлɟнɚɪиɫɭнкɟɊиɫɭнокɏɪомɚɬоɝɪɚммɚɫпиɪɬоɜоɝоизɜлɟчɟния P. fruticosa ɞɟɬɟкцияɞиоɞноэɮɮɟкɬиɜноɫɬиɪɚзɞɟлɟнияɫɭммыполиɮɟнолоɜпоɞоɛɪɚннойɯɪомɚɬоɝɪɚɮичɟɫкойɪɚɫɫчиɬɚныпɪиɝоɞноɫɬизɭльɬɚɬыкоɬоɪыɯпɪɟɞɫɬɚɜлɟныɬɚɛлицɟɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɌɚɛлицɚɊɟзɭльɬɚɬыɪɚɫчёɬоɜкɪиɬɟɪиɟɜэɮɮɟкɬиɜноɫɬиɯɪомɚɬоɝɪɚɮичɟɫкойɫиɫɬɟмыопɪɟɞɟлɟнияполиɮɟнолоɜ P. fruticosa L.3,40521731,320,880,17224,82280222,880,690,12676,94682994,040,690,185,938132051,350,70,121713,1082770713,070,620,185313,502675851,510,790,122215,657672254,370,810,142215,940415771,091,120,184016,6351710312,921,380,094716,852921621,150,710,130718,119561414,791,210,1918,6381447082,061,020,115319,0651274352,080,910,125719,3641168801,360,740,133323,85211656517,710,640,164425,1162537915,690,740,117327,8053334973,080,920,1133ɜɪɟмяɭɞɟɪжиɜɚниякомпонɟнɬɚчиɫлоɬɟоɪɟɬичɟɫкиɯɬɚɪɟлок ? коэɮɮициɟнɬɪɚзɞɟлɟнияпикоɜ ? эɮɮициɟнɬɚɫиммɟɬɪиипикɚɛɚзоɜойИɫɯоɞяполɭчɟнныɯɪɟзɭльɬɚɬоɜпɪɟɞɫɬɚɜлɟнɬɚɛлицɟ 2, эɮɮɟкɬиɜноɫɬиɜɚюɬɫяɪɟɮɟɪиɪɭɟмыɟзнɚчɟнияпɪɟɞлɚɝɚɟмыɟɋлɟɞоɜɚɬɟльноɯɪомɚɬоɝɪɚɮичɟɫкɭюможнопɪизнɚɬьэɮɮɟкɬиɜнойɊɚɫшиɮɪоɜкɚɪɟзɭльɬɚɬоɜɞиоɞномɚɬɪичноɝоɞɟɬɟкɬиɪоɜɚнияполиɮɟнолоɜP. fruticosa оɬɪɚжɟнɚɪиɫɭнкɟКɚкɫлɟɞɭɟɬпɪиɜɟɞённыɯɪиɫɭнкɟполиɮɟнольныйɫоɫɬɚɜɬɪɚɜыP. fruticosa пɪɟɞɫɬɚɜлɟнкɚɬɟɯинɚмиэллɚɝоɬɚннинɚмиэллɚɝоɜойкиɫлоɬойɮлɚɜоноиɞɚмиɝликозиɞɚмикɜɟɪцɟɬинɚкɟмпɮɟɪолɚɯлоɪоɝɟноɜойкиɫлоɬойПɪоцɟнɬноɟɪɚɫпɪɟɞɟлɟниɟполиɮɟнолоɜполиɮɟнольномкомплɟкɫɟP. fruticosa пɪɟɞɫɬɚɜлɟноɪиɫɭнкɟPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150220240260280300320340360380N"6406080100*DAD1, 3.408 (119 mAU, - ) Ref=3.188 & 3.715 of PENTAPHYLLOIDES70.DПɪоɚнɬоциɚниɞин220240260280300320340360380N"6100150200*DAD1, 4.821 (234 mAU, - ) Ref=4.555 & 5.321 of PENTAPHYLLOIDES70.DПɪоɚнɬоциɚниɞин220240260280300320340360380N"624681214*DAD1, 6.948 (14.9 mAU, - ) Ref=6.815 & 7.188 of PENTAPHYLLOIDES70.DЭллɚɝоɬɚннин220240260280300320340360380N"620304050607080*DAD1, 5.941 (83.6 mAU, - ) Ref=5.708 & 6.401 of PENTAPHYLLOIDES70.Dɏлоɪоɝɟноɜɚякиɫлоɬɚ240260280300320340360380N"624681214*DAD1, 11.650 (14.3 mAU, - ) Ref=11.343 & 12.603 of PENTAPHYLLOIDES.D ? 11,683Эллɚɝоɬɚннин220240260280300320340360380N"6246812*DAD1, 13.108 (12.0 mAU, - ) Ref=12.775 & 13.368 of PENTAPHYLLOIDES70.DȽликозиɞ220240260280300320340360380N"6468*DAD1, 13.501 (9.3 mAU, - ) Ref=13.368 & 13.715 of PENTAPHYLLOIDES70.DȽликозиɞ220240260280300320340360380N"620304050*DAD1, 15.655 (51.2 mAU, - ) Ref=15.508 & 15.775 of PENTAPHYLLOIDES70.DЭллɚɝоɜɚякиɫлоɬɚ220240260280300320340360380N"64681214*DAD1, 15.941 (12.8 mAU, - ) Ref=15.775 & 16.108 of PENTAPHYLLOIDES70.DȽликозиɞ220240260280300320340360380N"61234*DAD1, 16.635 (4.7 mAU, - ) Ref=16.515 & 16.708 of PENTAPHYLLOIDES70.DȽликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"620304050*DAD1, 16.855 (49.5 mAU, - ) Ref=16.708 & 17.175 of PENTAPHYLLOIDES70.DȽликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"6203040506070*DAD1, 18.121 (68.2 mAU, - ) Ref=17.835 & 18.488 of PENTAPHYLLOIDES70.D? 18,119Ƚликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"62345*DAD1, 18.635 (5.4 mAU, - ) Ref=18.501 & 18.768 of PENTAPHYLLOIDES70.DȽликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"612345*DAD1, 19.068 (5.3 mAU, - ) Ref=18.921 & 19.235 of PENTAPHYLLOIDES70.DȽликозиɞкɟмпɮɟɪолɚ220240260280300320340360380N"60.51.52.5*DAD1, 19.361 (2.7 mAU, - ) Ref=19.235 & 19.588 of PENTAPHYLLOIDES70.DȽликозиɞкɟмпɮɟɪолɚ220240260280300320340360380N"681214161820*DAD1, 23.855 (15.8 mAU, - ) Ref=23.601 & 24.408 of PENTAPHYLLOIDES70.DКɜɟɪцɟɬин220240260280300320340360380N"651520*DAD1, 25.115 (23.5 mAU, - ) Ref=24.955 & 25.488 of PENTAPHYLLOIDES70.D? 25,116Ⱦɟпɫиɞɮлɚɜоноиɞɚокɫикоɪичнойкиɫлоɬы220240260280300320340360380N"623456789*DAD1, 27.808 (8.4 mAU, - ) Ref=27.668 & 27.948 of PENTAPHYLLOIDES70.DКɟмпɮɟɪолɭɞɟɪжиɜɚниякомпонɟнɬɚɯɪомɚɬоɝɪɚммɟɊиɫɭнокɋоɫɬɚɜполиɮɟнольныɯɫоɟɞинɟний P. fruticosa L.ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɊиɫɭнокɪɚɫпɪɟɞɟлɟниɟполиɮɟнолоɜполиɮɟнольномкомплɟкɫɟ P. fruticosa L.КɚкпɪиɜɟɞённомɪиɫɭнкɟоɫноɜнɭюмɚɫɫɭполиɮɟнолоɜɬɪɚɜыP. fruticosa ɫоɫɬɚɜляюɬɝликозиɞыкɜɟɪцɟɬинɚкɚɬɟɯиныɯлоɪоɝɟноɜɚялоɬɚколичɟɫɬɜɚɯпɪиɫɭɬɫɬɜɭюɬɝликозикɟмпɮɟɪолɚɏɚɪɚкɬɟɪнымпɪизнɚкомпɪоɞɭкɬоɜкиɫлоɬноɝоɝиɞɪолизɚполиɮɟнолоɜɬɪɚɜыP. fruticosa яɜилоɫьчɬояɪкоокɪɚɫкɭчɬоɭкɚзыɜɚɟɬпɪиɫɭɬɫɬɜиɟɚнɬоциɚноɜȽиɞɪолизɚɬɞɚлɟɟɯɪомɚɬоɝɪɚɮиɪоɜɚлиɪɟзɭльɬɚɬыɯɪомɚɬоɝɪɚɮиɪоɜɚнияпɪиɜɟɞɟныɪиɫɭнкɚɯɊиɫɭнокɏɪомɚɬоɝɪɚммɚполиɮɟнолоɜ P. fruticosa ɝиɞɪолизɚɞɟɬɟкцияɞиоɞноPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Ɋиɫɭнокɏɪомɚɬоɝɪɚммɚполиɮɟнолоɜ P. fruticosa ɝиɞɪолизɚɞɟɬɟкцияɞиоɞноɊɟзɭльɬɚɬыɪɟзɭльɬɚɬоɜкиɫлоɬноɝоɝиɞɪолизɚполиɮɟнолоɜɬɪɚɜыP. fruticosa пɪиɜɟɞɟныɪиɫɭнкɟ225250275300325350375N"6203040506070*DAD1, 24.152 (72.3 mAU, - ) Ref=23.852 & 24.938 of PENTAPHYLLOIDESGIDR.DКɜɟɪцɟɬин225250275300325350375N"651520*DAD1, 28.018 (24.0 mAU, - ) Ref=27.712 & 28.645 of PENTAPHYLLOIDESGIDR.DКɟмпɮɟɪол225250275300325350375N"60.51.52.53.5*DAD1, 30.585 (4.3 mAU, - ) Ref=30.432 & 30.825 of PENTAPHYLLOIDESGIDR.DtR ? 30,585225250275300325350375N"6406080100*DAD1, 16.005 (114 mAU, - ) Ref=15.812 & 16.572 of PENTAPHYLLOIDESGIDR.DКиɫлоɬɚэллɚɝоɜɚя220240260280300320340360380N"65152025*DAD1, 10.932 (25.9 mAU, - ) Ref=10.658 & 11.138 of PENTAPHYLLOIDESGIDR.DКиɫлоɬɚɯлоɪоɝɟноɜɚя250300350400450500550N"6-2.52.57.51012.51517.5*DAD1, 14.638 (21.6 mAU, - ) Ref=14.458 & 15.145 of PENTAPHYLLOIDESGIDR.DɊиɫɭнокɋоɫɬɚɜпɪоɞɭкɬоɜɝиɞɪолизɚполиɮɟнолоɜ P. fruticosa L.ɌɚкимоɛɪɚзомпɪоɞɭкɬɚɯɝиɞɪолизɚɚɝликоныɮлɚɜонолоɜɫɭщɟɫɬɜɟннымпɪɟоɛлɚɞɚкɜɟɪцɟɬинɚɌɚкжɟиɞɟнɬиɮициɪоɜɚнчɬоɫлɭжиɬпоɞɬɜɟɪжɞɟниɟмнɚличияполиɮɟнолɚɯиɫɫлɟɞɭɟмоɝопɪоɚнɬоциɚниɞиноɜɯоɞɟнɚɫɬоящɟɝоиɫɫлɟɞоɜɚнияɭɫɬɚноɜлɟночɬокɭɪильɫкийɫоɞɟɪжиɬɮлɚɜоноиɞыпɪɟɞɫɬɚɜɝликозиɞɚмиɬɪёɯɚɝликоноɜ ? кɜɟɪцɟɬинɚкɟмпɮɟɪолɚпɪɟоɛлɚɞɚниɟмпɟɪɜоɝоПɪиɫɭɬɫɬɜиɟɮлɚɜоноиɞоɜоɛъɟкɬɟкɚкоɛɭɫлоɜлиɜɚɟɬмножɟɫɬɜоɮɚɪмɚколоɝичɟɫкиɯэɮɮɟкɬоɜкɚпилляɪоɭкɪɟпляɚнɬиокɫиɞɚнɬныйпɪоɬиɜоопɭɯолɟɜыйɪɟзɭльɬɚɬɟɯɪомɚɬоɝɪɚɮиɪоɜɚнияпɪоɞɭкɬоɜкиɫлоɬноɝоɝиɞɪолизɚполиɮɟнолоɜиɫɫлɟɞɭɟмоɝооɛъɟкɬɚчɬоɫоɞɟɪжиɬɫяɛольшоɟколичɟɫɬɜопɪоɚнɬоциɚниɞиноɜɝɪɭппыɫоɟɞиɯɚɪɚкɬɟɪизɭющиɯɫяɜыɪɚжɟннымиɚнɬиокɫиɫɜойɫɬɜɚмиЭɬонɟɫомнɟнноопɪɟɞɟляɟɬпɟɪɫпɟкɬиɜноɫɬькɚкиɫɬочникɚэɬойɝɪɭппыɜɟщɟɫɬɜКɪомɟɬоɝопɪоɞɭкɬɚɯɝиɞɪолиɭɫɬɚноɜлɟнопɪиɫɭɬɫɬɜиɟэллɚɝоɜойкиɫлоɬычɬоɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɭкɚзыɜɚɟɬпɪиɪоɞɭɞɭɛильныɯɜɟщɟɫɬɜэллɚɝоɬɚнниныЭллɚɝоɬɚнниныяɜляюɬɫяизɜɟɫɬпɪоɬиɜоɜоɫпɚлиɬɟльнымикɪоɜооɫɬɚнɚɜлиɜɚпɪоɬиɜоɬокɫичɟɫкимиɚɝɟнɬɚмиɋлɟɞоɜɚɞɟйɫɬɜɭющимнɚчɚломɬɪɚɜыP. fruticosa яɜляюɬɫяɬɪиклɚɫɫɚɫоɟɞинɟний ? ɮлɚɜоноиɞыɚнɬоциɚниɞиныэллɚɝоɬɚнниныНɚличиɟɬɚкоɝоɫпɟкɬɪɚполиɮɟнольныɯкомпонɟнɬоɜпозɜоляɟɬɭɬɜɟɪжɞɚɬьчɬоизɭчɟнныйоɛъɟкɬпɪɟɞɫɬɚɜляɟɬɫяпɟɪɫпɟкɬиɜнымиɫпользоɜɚниямɟɞицинɫкойпɪɚкɬикɟЭɬоɜозможноɫɬьɪɟкомɟнɞоɜɚɬьɞɚльнɟйшиɯɮɚɪмɚколоɝичɟɫкиɯиɫпыɬɚнийɪɚзɪɚɛоɬкилɟкɚɪɫɬɜɟнныɯпɪɟпɚɪɚɬоɜоɫноɜɟɟɝополиɮɟнольноɝокомплɟкɫɚЗȺКЛЮЧȿНИȿɪɟзɭльɬɚɬɟпɪоɜɟɞённыɯиɫɫлɟɞоɜɚнийɭɫɬɚноɜлɟночɬоɬɪɚɜɚP. fruticosa ɫоɞɟɪжиɬоколо 18 ɫоɟɞинɟнийполиɮɟнольнойɪоɞыпɪɟɞɫɬɚɜлɟнныɟɝликозиɞɚмиɚɝликонɚмиɮлɚɜонолоɜпɪоɚнɬоциɚниɞинɚмиокɫикоɪичнымилоɬɚмиэллɚɝоɬɚннинɚмиэɬомоколо 46% ɜɫɟйɫɭммыполиɮɟнолоɜɫоɫɬɚɜляюɬɮлɚɜоноиɞы, 24% ? пɪоɚнɬоциɚниɞиныокɫикоɪичныɟкиɫлоɬы, 7% эллɚɝоɬɚнниныПолɭчɟнныɟɪɟзɭльɬɚɬыпозɜоляюɬɭɬɜɟɪжɞɚɬьчɬоɫɬɚнɞɚɪɬизɚциюиɫɫлɟɞɭɟмоɝооɛъɟкможнопɪоɜоɞиɬьɫɭммɟполиɮɟнольныɯɫоɟɞипɟɪɟɫчёɬɟкɜɟɪцɟɬинкɚкɞоминиɪɭющɟɝокомпонɟнɬɚɫɭммыполиɮɟнолоɜпоɫлɟпɪɟɞɜɚɪиɬɟльноɝоɝиɞɪолизɚпɪоɚнɬоциɚниɞины L. L.) ? Kuril tea - is a plant from the Rosaceae family. The plant used to be referred to as the [1]. This is a popular plant in folk medicine in China, Tibet, Mongolia. Its owers owers diseases of the gastrointestinal tract, as a means to regu-late metabolism, menstruation. In Mongolian folk medi-cine it is used for diarrhea as a hemostatic [4, 5, 6]. This ammatory, antitumor and antiult-serogennye [4, 7, 8, 9, 10, 11, 12]. The current research cant antioxidant capacity in vitro and protective effect against Escherichia coli at Oxidative produced by phenolic components P. fruticosa L., [13, 14]. A comparative phytochemical study of three revealed the presence of hyperoside, catechin, caffeic and ferulic acid, rutin and ellagic acid in all of these objects. All of these species ex- and and A signi cant increase in the antioxidant activity of P. fruticosa fruticosa Chemical and pharmacological studies have shown that polyphenols of several classes, namely ? avonoids, P. fruticosaL. [17]. However, the content of pharmacologically sig- cant components in the plant composition can vary the growing region. This factor is re ected in the amount of ected in the amount of 23]. Accordingly, the evaluation of the qualitative com-P. fruticosa of this study P. fruticosa L. for standardizing its raw materials. The c direction direction MATERIALS AND METHODS. P. fruticosa L. herb collected in the Altai Territory was taken. The polyphenol complex from P. fruticosa L. herb was extracted with 70% ethyl alcohol at the ratio of 1:50 of raw materials to the extractant. The Agilent Technologies 1200 InAscentisexpress cation water and ethyl alcohol (according to GOST R 51652) were er. cient R and the asymmetry of the cient - was should be not more than - 1.5, and the asymmetry cient TTThe polyphenol complex was chromatographed under the following conditions:the mobile phase speed was 0,5 ml/min.;the column thermostat temperature was +35оɋ;the sample volume was 1 μl.The gradient elution was carried out under the conditions indicated in Table 1.Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Table 1 ? Conditions for the gradient elution of P. fruticosus L polyphenols Time, minA,%B,%09010108020207030305050 401090 avonols at 355 nm, hydroxycinnamic ac-avonols at 355 nm, hydroxycinnamic ac-The component composition was identi? ed by the avonoids aglucones, the acid hydrolysis of P. fruticosa polyphenols was carried out. The hydrolysis was 60 minutes. The resulting hydrolysate was ltered and RESULTS AND DISCUSSION. of the separation of alcohol extract from P. fruticosa L. herb is shown in Figure 1.Figure 1 ? Chromatograms of alcohol extract from herb P. fruticosa L.To assess the effectiveness of the separation of results of which are presented in Table 2.ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyTable 2 ? Results of calculations of ef ciency criteria of the chromatographic system for determination of P. fruticosa L. polyphenols 3.40521731.320.880.17224.82280222.880.690.12676.94682994.040.690.185.938132051.350.70.121713.1082770713.070.620.185313.502675851.510.790.122215.657672254.370.810.142215.940415771.091.120.184016.6351710312.921.380.094716.852921621.150.710.130718.119561414.791.210.1918.6381447082.061.020.115319.0651274352.080.910.125719.3641168801.360.740.133323.85211656517.710.640.164425.1162537915.690.740.117327.8053334973.080.920.1133where t is the component retention time, N is the number of theoretical plates, R is the peak separation factor, TBased on the results obtained in Table 2, the ef t into the referenced values proposed by the European Pharmacopoeia. Therefore, this chromato-graphic system can be considered effective. Decoding P. fruticosa ected in Figure 2. As follows from P. fruticosa L. herb is represented by catechines, avonoids, quercetin gly-P. fruticosa L. is shown in Figure 3.Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150220240260280300320340360380N"6406080100*DAD1, 3.408 (119 mAU, - ) Ref=3.188 & 3.715 of PENTAPHYLLOIDES70.DR*?3,405 Proanthocyanidin1220240260280300320340360380N"6100150200*DAD1, 4.821 (234 mAU, - ) Ref=4.555 & 5.321 of PENTAPHYLLOIDES70.DR ? 4,822Proanthocyanidin2220240260280300320340360380N"624681214*DAD1, 6.948 (14.9 mAU, - ) Ref=6.815 & 7.188 of PENTAPHYLLOIDES70.DR ? 6,946Ellagotannin220240260280300320340360380N"620304050607080*DAD1, 5.941 (83.6 mAU, - ) Ref=5.708 & 6.401 of PENTAPHYLLOIDES70.DR ? 5,938 Chlorogenic acid240260280300320340360380N"624681214*DAD1, 11.650 (14.3 mAU, - ) Ref=11.343 & 12.603 of PENTAPHYLLOIDES.D? 11,683220240260280300320340360380N"6246812*DAD1, 13.108 (12.0 mAU, - ) Ref=12.775 & 13.368 of PENTAPHYLLOIDES70.DR ? 13,108Glycoside oframnetine220240260280300320340360380N"6468*DAD1, 13.501 (9.3 mAU, - ) Ref=13.368 & 13.715 of PENTAPHYLLOIDES70.DR ? 13,502Glycoside of ramnetine220240260280300320340360380N"620304050*DAD1, 15.655 (51.2 mAU, - ) Ref=15.508 & 15.775 of PENTAPHYLLOIDES70.DR ? 15,657Ellagic acid220240260280300320340360380N"64681214*DAD1, 15.941 (12.8 mAU, - ) Ref=15.775 & 16.108 of PENTAPHYLLOIDES70.DR ? 15,940Glycoside oframnetine220240260280300320340360380N"61234*DAD1, 16.635 (4.7 mAU, - ) Ref=16.515 & 16.708 of PENTAPHYLLOIDES70.DtR ? 16,635Glycoside of quercetin220240260280300320340360380N"620304050*DAD1, 16.855 (49.5 mAU, - ) Ref=16.708 & 17.175 of PENTAPHYLLOIDES70.DR ? 16,852Glycoside of quercetin220240260280300320340360380N"6203040506070*DAD1, 18.121 (68.2 mAU, - ) Ref=17.835 & 18.488 of PENTAPHYLLOIDES70.D? 18,119220240260280300320340360380N"62345*DAD1, 18.635 (5.4 mAU, - ) Ref=18.501 & 18.768 of PENTAPHYLLOIDES70.DtR ? 18,638Glycoside of quercetin220240260280300320340360380N"612345*DAD1, 19.068 (5.3 mAU, - ) Ref=18.921 & 19.235 of PENTAPHYLLOIDES70.DtR ? 19,065Glycoside of kaempferol220240260280300320340360380N"60.51.52.5*DAD1, 19.361 (2.7 mAU, - ) Ref=19.235 & 19.588 of PENTAPHYLLOIDES70.DR ? 19,364Glycoside of kaempferol220240260280300320340360380N"681214161820*DAD1, 23.855 (15.8 mAU, - ) Ref=23.601 & 24.408 of PENTAPHYLLOIDES70.DR ? 23,852Quercetin220240260280300320340360380N"651520*DAD1, 25.115 (23.5 mAU, - ) Ref=24.955 & 25.488 of PENTAPHYLLOIDES70.D? 25,116 avonoid and 220240260280300320340360380N"623456789*DAD1, 27.808 (8.4 mAU, - ) Ref=27.668 & 27.948 of PENTAPHYLLOIDES70.Dis component retention time on the chromatogramFigure 2 ? Composition of P. fruticosa L polyphenolic compounds ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyFigure 3 ? Percentage distribution of polyphenols in the P. fruticosa L polyphenol complex gure, the main mass of P. fruticosaA characteristic feature of the products of acid P. fruticosa L. polyphenols was that they had a bright red color, indicating the presence of anthocyanins. The hydrolyzate was further Figure 4 ? Chromatogram of P. fruticosa L. polyphenols after acid hydrolysisPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Figure 5 ? Chromatogram of P. fruticosa L. polyphenols after acid hydrolysis225250275300325350375N"6203040506070*DAD1, 24.152 (72.3 mAU, - ) Ref=23.852 & 24.938 of PENTAPHYLLOIDESGIDR.DR ? 24,152Quercetin225250275300325350375N"651520*DAD1, 28.018 (24.0 mAU, - ) Ref=27.712 & 28.645 of PENTAPHYLLOIDESGIDR.DR ? 28,018Kaempferol225250275300325350375N"60.51.52.53.5*DAD1, 30.585 (4.3 mAU, - ) Ref=30.432 & 30.825 of PENTAPHYLLOIDESGIDR.DtR ? 30,585Ramnetine225250275300325350375N"6406080100*DAD1, 16.005 (114 mAU, - ) Ref=15.812 & 16.572 of PENTAPHYLLOIDESGIDR.DR ? 16,007Ellagic acid220240260280300320340360380N"65152025*DAD1, 10.932 (25.9 mAU, - ) Ref=10.658 & 11.138 of PENTAPHYLLOIDESGIDR.DR ? 10,932Chlorogenic acid250300350400450500550N"6-2.52.57.51012.51517.5*DAD1, 14.638 (21.6 mAU, - ) Ref=14.458 & 15.145 of PENTAPHYLLOIDESGIDR.DFigure 6 ? Composition of products of hydrolysis of P. fruticosa L. polyphenols avonols with a signi cant ed, which rms the presence of proanthocyanidins in the polyphenols of the plant under study.In the course of this study, it has been established avonoids represented by ramnetin, with predominance of quercetin. The presence avonoids in the object, as a rule, causes a variety of diverse pharmacological effects, including capillary-strengthening, antioxidant, antitumor.hydrolysis of the investigated object?s polyphenols, it was found out that the plant contains a large number characterized by a pronounced antioxidant effect. This, of course, determines the prospects of the plant as a source of this valuable group of substances. In addition, the presence of ellagic acid has been established in the products of hydrolysis, which indicates the nature of tannins of the plant ? ellagatannins. Elvolatannins are ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, Botany ammatory, hemostatic, antitoxic agents. Consequently, the principle of Kuril tea (P. fruticosa L.) is avonoids, proanthocyanidins and elligotannins. The presence of such a spectrum of valuable polyphenolic components in the plant makes it possible to assert that the studied object seems promising in medical practice. Therefore, it can be recommended for further pharmacological testing and for the development of Kuril tea (P. fruticosa L.) avonols, proanthocyanidins, oxycinnamic acids, ellagatannins. About 46% of the avonoids, 24% are are ellagatannins. The results obtained make it possible ȻиɛлиоɝɪɚɮичɟɫкийȾикоɪɚɫɬɭщиɟполɟзныɟɊоɫɫии / ɪɟɞȻɭɞɚнцɟɜЛɟɫиоɜɫкɚяИзɞɋПɏɎȺЛɚɜɪɟноɜɚПолнɚяэнциклопɟɞиялɟкɚɪɫɬɜɟнныɯОЛМȺɋоколоɜɎиɬоɬɟɪɚпияɮиɬоɮɚɪмɚколоɝияɊɭкоɜоɞɫɬɜоɜɪɚчɟйМɟɞицинɫкоɟинɮоɪмɚционноɟɚɝɟнɬɫɬɜоG?rb?z I., ?zkan A.M., Yesilada E., Kutsal O. Anti-ulcerogenic activity of some plants used in folk medicine of Pinarbasi (Kayseri, Turkey) // Journal of ethnopharmacology. 2005. No. 101. Feng Y., Wu Z.H., Zhou X., Zhou Z., Fan W. Knowledge discovery in traditional Chinese medicine: state of the art and perspectives // Artif. intell. med. 2006. No. 38. Liu Y.H., Sun Y.L. China traditional Chinese medicine patent database // World ȺɪьяɟɜɚȺжɭноɜɚНиколɚɟɜэкɫɬɪɚкɬɚпоɛɟɝоɜ Pentaphylloides fruticosa (L.) ɬɟчɟниɟэкɫпɟɪимɟнɬɚльноɝоɫɚɯɚɪноɝоɞиɚɛɟɬɚɪɟɫɭɪɫыȿɜɫɬɪопоɜȻɭɪоɜɚȽɪɟк O.P. полиɮɟнольноɝокомплɟкɫɚэкɫɬɪɚɝиɪоɜɚнноɝопяɬилиɫɬникɚкɭɫɬɚɪникоɜоɝо (Penthaphylloides fruticosa (L.) пɪоɮилɚкɬикиКокɫɚкиɪɭɫнойȻюллɟɬɟньɫиɛиɪɫкоймɟɞициныTomczyk M., Leszczyska K., Jakoniuk P. Antimicrobial activity of Potentilla species / M. Tomczyk, K. ska, P. Jakoniuk // Fitoterapia. 2008. Vol. 79. No. 7. Miliauskas G., van Beek T.A., Venskutonis P.R., Linssen J.P., Waard P., Sudholter E.JR. Antioxidant activity of Wang L.-Y., Kou Y.-X., Wu G.-L., Wang Y.-J. Development and characterization of novel microsatellite cation in its sister species - Tomczyk M., Paduch R., Wiater A., Pleszczyska M., Kandefer-Szersze M., Szczodrak J. The in uence of aqueous extracts of selected Potentilla species on normal human colon cells // Actapoloniaepharmaceutica. Drug Miliauskas G., Mulder E., Linssen J.PH, Houben J.H., van Beek T.A., Venskutonis P.R. Evaluation of antioxidative Luo Z., Wang S., Wang D. Phenolic pro les and antioxidant capacities of crude extracts and subsequent fractions Wang S., Wang D., Pu W., Li D. Phytochemical pro les, antioxidant and antimicrobial activities of three Potentilla Liu Z., Luo J.C., Wang D. Synergistic effects of Potentilla fruticose L. leaves combined with green tea polyphenols in a variety of oxidation systems // Journal of food science. No. 81. P. 1091?1101.Chen C., Zhang X.W., Luo Z.M. Measurement of quercetin and kaempferol contents in Potentilla fruticosa from different regions by HPLC method // Chinese journal of analysis laboratory. 2009. ɏɪɚмоɜɚȾинɚмикɚɫоɞɟɪжɚнияɮлɚɜонолоɜнɚɞзɟмныɯ Pentaphylloides fruticosa (L.) O. экоɬипоɜНоɜоɫиɛиɪɫкɟɪɟɫɭɪɫыɏɪɚмоɜɚɋоɫɬɚɜɫоɞɟɪжɚниɟɮлɚɜоноиɞоɜ Pentaphylloides fruticosa пɪиɪоɞɟкɭльɬɭɪɟ // ɪɚɫɬиɬɟльноɝоɏɪɚмоɜɚОɫоɛɟнноɫɬинɚкоплɟнияɮɟнольныɯɫоɟɞинɟний Potentilla fructose (Rosaceae) ɬɟчɟнииɫɭɬокɪɚɫɬиɬɟльноɝоBai D.Y., Ma M.C., Zhang Z.H. Analysis of leaf ingredient in wild Potentilla fruticosa L. of different elevation // Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Li H.C., Sun H.Z., Hu X. Analysis on total avonoid in leaves of Potentilla fruticosa in different environment and Tomczyk M., Pleszczynska M., Wiater A. Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity // Molecules. 2010. Vol. 15. No. 7. ПиɫɚɪɟɜНоɜикоɜɋɟɜɪɭкМɚлюɬинɚЗинчɟнкоȽɭɪьɟɜИɫɫлɟɞоɜɚниɟɪɚмкɚɯнɚɭчноɝонɚпɪɚɜлɟнияɎɚɪмɚцɟɜɬичɟɫкийНɚɭчныйɪɟзɭльɬɚɬɋɟɬɟɜойнɚɭчпɪɚкɬɋɟɪМɟɞицинɚɎɚɪмɚцияStrasbourg, France: 2727.Markham K.R., Bloor S.J. Analysis and identi cation of avonoids in practice // Flavonoids in health and disease / ed. by C. A. Rice-Evans, L. Packer. New York, 1998. P. 1-34. (Antioxidants in health and disease).ReferencesBudantsev AL, Lesiovskaya EE, editors. Dikorastushchiye poleznyye rasteniya Rossii [Wild plants of Russia]. St. Petersburg: SPFhA Publishing House;2001. 663 p. Russian.Lavrenov VK, Lavrenova GV. Polnaya entsiklopediya lekarstvennykh rasteniy. [Complete encyclopedia of me-dicinal plants]. Vol. 1. Moscow: OLMA-PRESS;1999. 736 p. Russian.Sokolov SYa. Fitoterapiya i tofarmakologiya: Rukovodstvo dlya vrachey [Phytotherapy and phytopharmacolo-gy: A guide for doctors]. Moscow: Medical News Agency;2000. 976 p. Russian. AM, Yesilada E, Kutsal O. 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Fitoterapia. 2008;79(7):Miliauskas G, van Beek TA, Venskutonis PR, Linssen JP, Waard P, Sudholter EJR. Antioxidant activity of Poten-Wang L-Y, Kou Y-X, Wu G-L, Wang Y-J. Development and characterization of novel microsatellite markers cation in its sister species ? Potentilla Tomczyk M, Paduch R, Wiater A, ska M, Kandefer-Szersze M, Szczodrak J. uence of aqueous extracts of selected Potentilla species on normal human colon cells. Actapoloniaepharmaceutica. Drug research. Miliauskas G, Mulder E, Linssen JPH, Houben JH, van Beek TA, Venskutonis PR. Evaluation of antioxidative Luo Z, Wang S, Wang D. Phenolic pro les and antioxidant capacities of crude extracts and subsequent fractions Wang S, Wang D, Pu W, Li D. Phytochemical pro les, antioxidant and antimicrobial activities of three Potentilla Liu Z, Luo JC, Wang D. Synergistic effects of Chen C, Zhang XW, Luo ZM. Measurement of quercetin and kaempferol contents in from different regions by HPLC method. Chinese journal of analysis laboratory. 2009;28:54?6.Khramova EP. Dinamika soderzhaniya avonolov v nadzemnykh organakh Pentaphylloides fruticosa (L.) O. Schwarz. razlichnykh ekotipov, vyrashchennykh v Novosibirske [Dynamics of the content of avonols in the aerial organs Pentaphylloides fruticosa (L.) O. Schwarz. different ecotypes grown in Novosibirsk]. Rastitelnye resursy. 1999;35(4):31?8. Russian.Khramova EP. Sostav i soderzhaniye avonoidov Pentaphylloides fruticosa v prirode i kul?ture [Composition avonoids Pentaphylloides fruticosa in nature and culture]. Chemistry of plant raw materials. ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyKhramova EP. Osobennosti nakopleniya fenol?nykh soyedineniy v rasteniyakh Potentilla fructose (Rosaceae) Bai DY, Ma MC, Zhang ZH. Analysis of leaf ingredient in wild L. of different elevation avonoid in leaves of in different environment Tomczyk M, Pleszczynska M, Wiater A. Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity. Molecules. 2010;15(7):4639?4651.Pisarev DI, Novikov OO, Sevruk IA, Malutina AU, Zinchenko AA, Guriev IV. Issledovaniye Ocimum basilicum . Issledovaniye Ocimum basilicum Ocimum basilicum L. within the framework of the scienti? c direction ?Pharmaceutical remake?]. Nauchnyy rezul?tat. Setevoy nauch.-prakt. zhurn. Ser. Meditsina. Farmatsiya. 2016;2(2):54?61. Russian.bourg, France: 2727.Markham KR, Bloor SJ. Analysis and identi cation of avonoids in practice. Flavonoids in health and disease. . New York; 1998. 1-34. (Antioxidants in health and disease). Con ict of interest ict of interest.Malyutina Anastasia Yuryevna ? PhD (Pharmacy)assistant professor of the Department of Pharmaceutical cational Establishment of Higher Professional Education of Belgorod State University, Ministry of Education and Science of the Russian Federation. Research interests: y, pharmacognosy. E-mail: ma-Pravlotskaya Alina Sergeevna ? post-graduate stu-lishment of Higher Professional Education of Belgorod State University, Ministry of Education and Science of Russia. Research interests: pharmaceutical chemistry, pharmacognosy. E-mail: 1163773@bsu.edu.ru ORCID: ? PhD (Pharmacy), Profes-sor, Head of the Department of Pharmaceutical Chem-tional Establishment of Higher Professional Education of Belgorod State University, Ministry of Education and Science of Russia. Research interests: pharmaceutical chemistry, pharmacognosy. PhD (Pharmacy), Associ-ate Professor, Professor of the Department of Pharmaceuti-ucational Establishment of Higher Professional Education of Belgorod State University, Ministry of Education and Sci-Research interests: phar-maceutical chemistry, pharmacognosy. E-mail: pisarev@Sent back for revision: 03.04.2018КонɮликɬинɬɟɪɟɫоɜȺɜɬоɪызɚяɜляюɬоɬɫɭɬɫɬɜииконɮликɬɚинɬɟɪɟɫоɜМɚлюɬинɚ ? кɚнɞиɞɚɬɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкɞоцɟнɬкɚɮɟɞɪыɮɚɪмɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииОɛлɚɫɬьнɚɭчныɯɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозия, ORCID: 0000-0001-6170-2151.Пɪɚɜлоцкɚяɋɟɪɝɟɟɜнɚ ? ɮɟɞɪыɮɚɪмɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫиилɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозияE-mail: 1163773@bsu.edu.ru, ORCID: Ноɜикоɜ ? ɞокɬоɪɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкпɪоɮɟɫɫоɪзɚɜɟɞɭющийкɚɮɟɞɪоймɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииОɛлɚɫɬьнɚɭчинɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозияПиɫɚɪɟɜИɜɚноɜич ? ɞокɬоɪɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкɞоцɟнɬпɪоɮɟɫɫоɪкɚɮɟɞɪымɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииОɛлɚɫɬьнɚɭчинɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозия. E-mail: pisarev@bsu.edu.ru, ПоɫɬɭпилɚɪɟɞɚкциюОɬпɪɚɜлɟнɚɞоɪɚɛоɬкɭПɪиняɬɚпɟчɚɬиPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150ɍȾК 577.13:582.71: 543.544 ʰʯ˄ˋʫʻʰʫКОʺʿОʻʫʻ˃ʻОʧОˁОˁ˃ʤʦʤʿОЛʰˇʫʻОЛОʦ˃ˀʤʦˏPENTAPHYLLOIDES FRUTICOSAМɚлюɬинɚПɪɚɜлоцкɚяНоɜикоɜПиɫɚɪɟɜɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииɊоɫɫияȻɟлɝоɪоɞПоɛɟɞыКɭɪильɫкийяɜляɟɬɫяпопɭляɪнымɪɚɫɬɟниɟмнɚɪоɞноймɟɞициныКиɬɚяМонɝолииИмɟющиɟɫянɚɭчныɟɫɜɟɞɟнияɫɜиɞɟɬɟльɫɬɜɭюɬɜыɪɚжɟнныɯɚнɬиокɫиɞɚнɬныɯɫɜойɫɬɜɫопɪянɚличиɟмполиɮɟнолоɜɌɚкжɟɭɫɬɚноɜлɟнɪяɞɞɪɭɝиɯɮɚɪмɚколоɝичɟɫкиɯэɮɮɟкɬоɜɚнɬиɛɚкɬɟɪиɚльныйɮɭнɝициɞныйɝипоɝликɟмичɟɫкийпɪоɬиɜоɜоɫпɚлиɬɟльныйпɪоɬиɜоязɜɟнОɞнɚкоɫоɞɟɪжɚниɟполиɮɟнолоɜɫоɫɬɚɜɟɜɚɪьиɪɭɟɬɝɟолоɝичɟɫкойзоныкоɬоɪойпɪоизɪɚɫɬɚлооɬɪɚжɚɟɬɫяɬɟɪɚпɟɜɬичɟɫкойɚкɬиɜноɫɬиПоэɬомɭцɟльюнɚɫɬоящɟɝоиɫɫлɟɞоɜɚнияяɜилоɫьизɭчɟниɟкɚчɟɫɬɜɟнноɝоɫоɫɬɚɜɚполиɮɟнолоɜ P. fruticosa ɫɬɚнɞɚɪɬизɚцииМɚɬɟɪиɚлымɟɬоɞыкɚчɟɫɬɜɟоɛъɟкɬɚиɫɫлɟɞоɜɚнияɜзяɬоɛɪɚзɟц P. fruticosa L., ɫоɛɪɚнныйȺлɬɚйɫкоɝопɪоɜоɞилɫяпомощьюɜыɫокоэɮɮɟкɬиɜнойжиɞкоɫɬнойɯɪомɚɬоɝɪɚɮииоɛɪɚщённоɮɚзномɜɚɪиɚнɬɟɊɟзɭльɬɚɬыоɛɫɭжɞɟɍɫɬɚноɜлɟнɯимичɟɫкийɫоɫɬɚɜкоɬоɪыйɜключɚɟɬ 18 ɫоɟɞинɟнийполиɮɟнольнойпɪиɪоɞыпɪɟɞɫɬɚɜлɟнныйɝликозиɞɚмиɚɝликонɚмиɮлɚɜонолоɜокɫикоɪичнымикиɫлоɬɚмиэллɚɝоɬɚннинɚмиМɟɬоɞомноɪмɚлизɚцииɪɚɫɫчиɬɚнооɬноɫиɬɟльноɟɫоɞɟɪжɚниɟкɚжɞоɝокомпонɟнɬɚɫɭммɟɪɟзɭльɬɚɬɟɭɫɬɚноɜлɟнопɪɟоɛлɚɞɚющимиɝликозиɞыкɜɟɪцɟɬинɚОпɪɟɞɟлёнɫоɫɬɚɜɚɝликоноɜɮлɚɜонолоɜпоɫлɟкиɫлоɬноɝоɝиɞɪолизɚɜключɚющийкɜɟɪцɟɬинкɟмпɮɟɪолɪɚмнɟɬинпɪɟоɛлɚɞɚниɟмпɟɪɜоɝокомпонɟнɬоɜкиɫлоɬноɝоɝиɞɪолизɚокɚзɚлɫяяɜляɟɬɫяɜɟɫкимɞокɚзɚɬɟльɫɬɜомɏɚɪɚкɬɟɪноɞоɫɬɚɬочноɜыɫокоɟɫоɞɟɪжɚниɟɫоɫɬɚɜиɜшɟɟоколо 24% оɛщɟйɫɭммыполиɮɟнолоɜЗɚключɟПолɭчɟнныɟɪɟзɭльɬɚɬыпозɜоляюɬɭɬɜɟɪжɞɚɬьɫɬɚнɞɚɪɬизɚциюиɫɫлɟɞɭɟмоɝооɛъɟкɬɚможнопɪоɜоɞиɬьпɟɪɟɫчёɬɟкɜɟɪцɟɬинкɚкɞоминиɪɭющɟɝокомпонɟнɬɚɫɭммыполиɮɟнолоɜпоɫлɟпɪɟɞɜɚɪиɬɟльноɝоɝиɞɪолизɚКлючɟɜыɟɫлоɜɚкɭɪильɫкийɜыɫокоэɮɮɟкɬиɜнɚяжиɞкоɫɬнɚяɯɪомɚɬоɝɪɚɮияɮлɚɜоноиɞыциɬиɪоɜɚнияМɚлюɬинɚПɪɚɜлоцкɚяНоɜикоɜПиɫɚɪɟɜИЗɍЧȿНИȿКОМПОНȿНɌНОȽОɋОɋɌȺȼȺПОЛИɎȿНОЛОȼɌɊȺȼЫPENTAPHYLLOIDES FRUTICOSAɎɚɪмɚцияɮɚɪмɚколоɝияМɚлюɬинɚПɪɚɜлоцкɚяНоɜикоɜПиɫɚɪɟɜSTUDY OF THE COMPONENT COMPOSITION OF POLYPHENOLES OF THE KURIL TEAPLANT PENTAPHYLLOIDES FRUTICOSAA.Yu. Malyutina, A.V. Pravlotskaya, O.O. Novikov, D.I. Pisarev Belgorod National State Research University of the Russian Federation, 85, Pobeda Str., Belgorod, Russia, 308015 c evidence indicates the presence of the plant expressed antioxidant properties, conjugated with the presence of polyphenols. ammatory and antiulcer. However, the content of polyphenols in the plant varies greatly depending on the geological growing region, which affects its therapeutic activity. Therefore, of this research was to study the For citation:Malyutina A.Yu., Pravlotskaya A.V., STUDY OF THE COMPONENT COMPOSITION OF POLYPHENOLES OF THE KURIL PLANT (PENTAPHYLLOIDES FRUTICOSAPharmacy & Pharmacology.2018;6(2):135-150. DOI: 10.19163/2307-9266-2018-6-2-135-150ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, Botanyкɭɪильɫкийɫɟмɟйɫɬɜɚоɬноɫилиɪоɞɭоɞнɚконɚɫɬоящɟɟɟɝоɫмɚɬɪиɜɚюɬкɚкпɪɟɞɫɬɚɜиɬɟльɪоɞɚ. ЭɬопопɭляɪноɟнɚɪоɞноймɟɞицинɟКиɬɚяɌиɛɟɬɚМонɝолииɋɜоёнɚзɜɚниɟполɭчилɬоɝочɬоɟɝоцɜɟɬкиɜыɫɭшɟнномиɫпользоɜɚликɚкнɚɪоɞыпɪожиɜɚющиɟɪиɬоɪииɍɪɚлɚКɭɪильɫкиɯоɫɬɪоɜоɜ [2, 3]. КиɬɚɟэɬоиɫпользɭюɬзɚɛолɟɜɚнияɯжɟлɭɞочкишɟчноɝоɬɪɚкɬɚкɚкɫɪɟɞɫɬɜоɪɟɝɭлиɪɭющɟɟмɟɬɚɛолизммɟнɫɬɪɭɚцииМонɝольɫкойнɚɪоɞноймɟɞицинɟпɪимɟняɟɬɫякɚккɪоɜооɫɬɚнɚɜлиɜɚющɟɟ [4, 5, 6]. нɚɫɬоящɟɝокɚкɞɪɭɝиɯпɪɟɞɫɬɚɜиɬɟлɟйɪоɞɚɯɚɪɚкɬɟɪɟнɜɚжныɯɮɚɪмɚколоɝичɟɫкиɯɫɜойɫɬɜɬɚкиɯкɚкɚнɬиокɫиɞɚнɬныɟпɪоɬиɜомикɪоɛныɟпɪоɬиɜоɜиɪɭɫныɟɝипоɝликɟмичɟɫкиɟпɪоɬиɜоɜоɫпɚлиɬɟльпɪоɬиɜоопɭɯолɟɜыɟɚнɬиɭльцɟɪоɝɟнныɟ [4, 7, 8, 9, 10, 11, 12]. нɚɭчныɟиɫɫлɟɞоɜɚɫɜиɞɟɬɟльɫɬɜɭюɬчɬоɮɟнольныɟкомпонɟнɬыP. L., пɟɪɜɭюочɟɪɟɞьэллɚɝоɜɚякиɫлоɬɚ (+)-кɚɬɟɯинокɚзыɜɚюɬзнɚчиɬɟльнɭюɬиокɫиɞɚнɬнɭюɫпоɫоɛноɫɬьin vitroпɪоɬɟкɬиɜныйэɮɮɟкɬоɬношɟнииокɫиɞɚционномɫɬɪɟɫɫɟ [13, 14]. ɋɪɚɜниɬɟльноɟɮиɬоɯимичɟɫкоɟизɭчɟниɟɬɪɟɯпокɚзɚлочɬоɜɫɟɯпɟɪɟчиɫлɟнныɯоɛъɟкɬɚɯпɪиɫɭɬɫɬɜɭюɬкɚɬɟɯинкоɮɟйнɚяɮɟɪɭлоɜɚякиɫлоɬыэллɚɝоɜɚякиɫлоɬɚȼɫɟпɟɪɟчиɫлɟнныɟпɪояɜлялиɚнɬиокɫиɞɚнɬныɟпɪоɬиɜомикɪоɛныɟɫɜойɫɬɜɚоɬношɟнииɝɪɚмположиɬɟльныɯɛɚкɬɟɪийɬɚкжɟоɬмɟчɟнознɚчиɬɟльноɟɚнɬиокɫиɞɚнɬнойɚкɬиɜноɫɬиэкɫɬɪɚкɬоɜP. L.комɛинɚцииполиɮɟнолɚмизɟлɟноɝоɫооɬношɟнииɫооɬɜɟɬɫɬɜɟнноɏимичɟɫкиɟɮɚɪмɚколоɝичɟɫкиɟиɫɫлɟɞоɜɚпокɚзɚличɬоɜыɫокɭюɚнɬиокɫиɞɚнɬнɭюɬиɜноɫɬьP. fruticosa оɬɜɟɬɫɬɜɟнныполиɮɟнолынɟɫколькиɯɮлɚɜоноиɞыɜоɞныɟкɜɟɪцɟɬинɚкɟмпɮɟɪолɚɞɭɛильныɟщɟɫɬɜɚ [17]. ОɞнɚкоɫоɞɟɪжɚниɟɮɚɪмɚколоɝичɟɫкизнɚчимыɯкомпонɟнɬоɜɫоɫɬɚɜɟɜɚɪьиɪоɜɚɬьзɚɜиɫимоɫɬиэколоɝоцɟноɬичɟɫкиɯпɪоизɪɚɫɬɚнияЭɬооɬɪɚжɚɟɬɫяколичɟɫɬɜɟɛиолоɝичɟɫкиɚкɬиɜныɯɫоɟɞинɟнийкɚкɫлɟɞɫɬɜиɟɬɟɪɚпɟɜɬичɟɫкойɬиɜноɫɬи [18, 19, 20, 21, 22, 23]. эɬойɫɜязиоцɟнкɚкɚчɟɫɬɜɟнноɝоɫоɫɬɚɜɚколичɟɫɬɜɟнноɟɫоɞɟɪжɚɬɪɚɜɟP. fruticosa яɜляɟɬɫяɚкɬɭɚɛлɟмойɍчиɬыɜɚяɜышɟɫкɚзɚнноɟЦȿЛЬЮнɚɫɬоящɟɝоиɫɫлɟɞоɜɚнияяɜилоɫьизɭчɟниɟкɚчɟɫɬɜɟнноɝоɫоɫɬɚполиɮɟнолоɜɬɪɚɜыP. fruticosa ɫɬɚнɞɚɪɬизɚИзɭчɟниɟɞɚнноɝопɪоɜоɞиɬɫяɪɚмкɚɯноɜоɝонɚɭчноɝонɚпɪɚɜлɟниямɚцɟɜɬичɟɫкийМȺɌȿɊИȺЛЫМȿɌОȾЫкɚчɟɫɬɜɟоɛъɟкɬɚиɫɫлɟɞоɜɚнияɬɪɚɜыP. fruticosa L.ɬɟɪɪиɬоɪииȺлɬɚйɫкоɝоПолиɮɟнольныйкомплɟкɫɬɪɚɜыP. fruticosa изɜлɟкɚлипомощьюɫпиɪɬɚэɬилоɜоɝо 70%-концɟнɬɪɚцииɫооɬношɟнии 1:50 экɫɬɪɚɝɟнɬɭПолɭчɟнноɟизɜлɟчɟниɟɯɪомɚɬоɝɪɚɮиɪоɜɚлипɪɟɞɫɬɚɜлɟнныɯнижɟȺнɚлизпɪоɜоɞилɫяпомощьюɜыɫокоэɮɮɟкɬиɜжиɞкоɫɬнойɯɪомɚɬоɝɪɚɮииномɜɚɪиɚнɬɟɏɪомɚɬоɝɪɚɮиɪоɜɚлиɯɪомɚɬоɝɪɚɮɟɮиɪмыAgilent Technologies 1200 Inɏɪомɚɬоɝɪɚɮичɟɫкɚяколонкɚ ? Ascentisexpress кɚчɟɫɬɜɟиɫпользоɜɚныɜоɞɚкɜɚлиɮикɚции HPLC ɫпиɪɬȽОɋɌкиɫлоɬныммоɞиɮикɚɬоɪомɫлɭжилɚкиɫлоɬɚɊɚɫчɟɬɬɟоɪɟɬичɟɫкиɯɬɚɪɟлокɪɚзɞɟляɫпоɫоɛноɫɬиɯɪомɚɬоɝɪɚɮичɟɫкойкоэɮɮициɟнɬɪɚзɞɟлɟнияɚɫиммɟɬɪиюмɚɬоɝɪɚɮичɟɫкоɝопикɚ ? коэɮɮициɟнɬɚɫиммɟɬɪииɪɚɫɫчиɬыɜɚлиɫоɝлɚɫноȿɜɪопɟйɫкойɎɚɪмɚкопɟɟȺɞɟкɜɚɬнымизнɚчɟниямиɬɟоɪɟɬичɟɫкиɯзнɚчɟнийпɪиняɬо 5000, коэɮɮициɟнɬɚɛолɟɟ ? 1,5, коэɮɮициɟнɬɚɚɫиммɟɬɪииqualitative composition of polyphenols in P. fruticosa L. for further standardization of its raw materials. As a research object, a sample of P. fruticosa L. herb collected in the Altai Territory was taken. The analysis was carried out using high-performance liquid chromatography in the reversed-phase version. Results and nature, represented by glycosides and aglycons avonols, proanthocyanidins, oxycinnamic acids, ellagatannins. By the method of internal normalization, the relative content of each component in the sum is has been calculated. As a result, glycosides of quercetin have been found to prevail. After acid hydrolysis the composition of aglycon avonols was determined. It included quercetin, kaempferol and ramnetin, with predominance of quercetin. One of the components of acid hydrolysis was cyanidine, which is a strong proof of the presence of proanthocyanidins in the plant. A fairly high content of proanthocyanidins amounting to about 24% of the total amount of polyphenols is typical of it. The obtained results make it possible to assert that standardization of the investigated object can be carried out in terms of quercetin as the dominant component of the sum of polyphenols after preliminary hydrolysis.Kuril tea (Potentilla fruticosa L.), high-performance liquid chromatography, prolanthocyanidinsPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150ПолиɮɟнольныйкомплɟкɫɯɪомɚɬоɝɪɚɮиɪоɜɚлиɫлɟɞɭющиɯɫкоɪоɫɬьɬɟмпɟɪɚɬɭɪɚɬɟɪмоɫɬɚɬɚколонкиоɛъёмȽɪɚɞиɟнɬныйэлюиɪоɜɚнияпɪоɜоɞилиɭкɚзɚнныɯɬɚɛлицɟɌɚɛлицɚɍɫлоɜияэлюиɪоɜɚнияполиɮɟнолоɜ P. fruticosus LминȺ09010108020207030305050 401090Ɋɟɝиɫɬɪɚциюкомпонɟнɬоɜпɪоɜоɞилиɫлɟɞɭɜолнɮлɚɜонолы ? 355 ɝиɞɪокɫикоɪичныɟкиɫлоɬы ? 310, 325 пɪоɚнɬоциɚниɞиныэллɚɝоɬɚнниныКомпонɟнɬныйɫоɫɬɚɜиɞɟнɬиɮициɪоɜɚлиɫооɬɜɟɬɫɬɜиюɭɞɟɪжиɜɚнияɚнɚлиɬоɜɫɬɚнɞɚɪɬнымиɬɚкжɟɪɟзɭльɬɚɬɚмɞиоɞмɚɬɪичноɝоɞɟɬɟкɬиɪоɜɚнияОɬноɫиɬɟльноɟɫоɞɟɪжɚниɟоɬɞɟльныɯкомпонɟнɬоɜопɪɟɞɟлялимɟɬоɞомɜнɭɬɪɟннɟйноɪмɚлизɚɪɚɫчёɬɜɟлиɮоɪмɭлɟ:ɝɞɟ Si ? ɫɪɟɞнɟɟзнɚчɟниɟпикɚкомпонɟнɬɚɯɪомɚɬоɝɪɚммɚɯɫɭммыɫɪɟɞнɟɟзнɚчɟниɟɫɭммыɜɫɟɯкоɜɯɪомɚɬоɝɪɚммɚɯоɛъɟкɬиɜнойɫоɞɟɪжɚнияɚɝликоноɜɮлɚɜоноиɞоɜпɪоɜɟɞёнкиɫлоɬныйɝиɞɪолизлиɮɟнолоɜɬɪɚɜыP. fruticosa Ƚиɞɪолизпɪоɜоɞилиɪɚɫɬɜоɪомкиɫлоɬыɯлоɪиɫɬоɜоɞоɪоɞнойɬɟчɟ 60 ПолɭчɟнныйɝиɞɪолизɚɬɮильɬɪоɜɚлиɯɪомɚɬоɝɪɚɮиɪоɜɚлиɊȿЗɍЛЬɌȺɌЫОȻɋɍЖȾȿНИȿɏɪомɚɬоɝɪɚммɚɪɚзɞɟлɟнияɫпиɪɬоɜоɝоизɜлɟчɟнияɬɪɚɜыP. fruticosa пɪɟɞɫɬɚɜлɟнɚɪиɫɭнкɟɊиɫɭнокɏɪомɚɬоɝɪɚммɚɫпиɪɬоɜоɝоизɜлɟчɟния P. fruticosa ɞɟɬɟкцияɞиоɞноэɮɮɟкɬиɜноɫɬиɪɚзɞɟлɟнияɫɭммыполиɮɟнолоɜпоɞоɛɪɚннойɯɪомɚɬоɝɪɚɮичɟɫкойɪɚɫɫчиɬɚныпɪиɝоɞноɫɬизɭльɬɚɬыкоɬоɪыɯпɪɟɞɫɬɚɜлɟныɬɚɛлицɟɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɌɚɛлицɚɊɟзɭльɬɚɬыɪɚɫчёɬоɜкɪиɬɟɪиɟɜэɮɮɟкɬиɜноɫɬиɯɪомɚɬоɝɪɚɮичɟɫкойɫиɫɬɟмыопɪɟɞɟлɟнияполиɮɟнолоɜ P. fruticosa L.3,40521731,320,880,17224,82280222,880,690,12676,94682994,040,690,185,938132051,350,70,121713,1082770713,070,620,185313,502675851,510,790,122215,657672254,370,810,142215,940415771,091,120,184016,6351710312,921,380,094716,852921621,150,710,130718,119561414,791,210,1918,6381447082,061,020,115319,0651274352,080,910,125719,3641168801,360,740,133323,85211656517,710,640,164425,1162537915,690,740,117327,8053334973,080,920,1133ɜɪɟмяɭɞɟɪжиɜɚниякомпонɟнɬɚчиɫлоɬɟоɪɟɬичɟɫкиɯɬɚɪɟлок ? коэɮɮициɟнɬɪɚзɞɟлɟнияпикоɜ ? эɮɮициɟнɬɚɫиммɟɬɪиипикɚɛɚзоɜойИɫɯоɞяполɭчɟнныɯɪɟзɭльɬɚɬоɜпɪɟɞɫɬɚɜлɟнɬɚɛлицɟ 2, эɮɮɟкɬиɜноɫɬиɜɚюɬɫяɪɟɮɟɪиɪɭɟмыɟзнɚчɟнияпɪɟɞлɚɝɚɟмыɟɋлɟɞоɜɚɬɟльноɯɪомɚɬоɝɪɚɮичɟɫкɭюможнопɪизнɚɬьэɮɮɟкɬиɜнойɊɚɫшиɮɪоɜкɚɪɟзɭльɬɚɬоɜɞиоɞномɚɬɪичноɝоɞɟɬɟкɬиɪоɜɚнияполиɮɟнолоɜP. fruticosa оɬɪɚжɟнɚɪиɫɭнкɟКɚкɫлɟɞɭɟɬпɪиɜɟɞённыɯɪиɫɭнкɟполиɮɟнольныйɫоɫɬɚɜɬɪɚɜыP. fruticosa пɪɟɞɫɬɚɜлɟнкɚɬɟɯинɚмиэллɚɝоɬɚннинɚмиэллɚɝоɜойкиɫлоɬойɮлɚɜоноиɞɚмиɝликозиɞɚмикɜɟɪцɟɬинɚкɟмпɮɟɪолɚɯлоɪоɝɟноɜойкиɫлоɬойПɪоцɟнɬноɟɪɚɫпɪɟɞɟлɟниɟполиɮɟнолоɜполиɮɟнольномкомплɟкɫɟP. fruticosa пɪɟɞɫɬɚɜлɟноɪиɫɭнкɟPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150220240260280300320340360380N"6406080100*DAD1, 3.408 (119 mAU, - ) Ref=3.188 & 3.715 of PENTAPHYLLOIDES70.DПɪоɚнɬоциɚниɞин220240260280300320340360380N"6100150200*DAD1, 4.821 (234 mAU, - ) Ref=4.555 & 5.321 of PENTAPHYLLOIDES70.DПɪоɚнɬоциɚниɞин220240260280300320340360380N"624681214*DAD1, 6.948 (14.9 mAU, - ) Ref=6.815 & 7.188 of PENTAPHYLLOIDES70.DЭллɚɝоɬɚннин220240260280300320340360380N"620304050607080*DAD1, 5.941 (83.6 mAU, - ) Ref=5.708 & 6.401 of PENTAPHYLLOIDES70.Dɏлоɪоɝɟноɜɚякиɫлоɬɚ240260280300320340360380N"624681214*DAD1, 11.650 (14.3 mAU, - ) Ref=11.343 & 12.603 of PENTAPHYLLOIDES.D ? 11,683Эллɚɝоɬɚннин220240260280300320340360380N"6246812*DAD1, 13.108 (12.0 mAU, - ) Ref=12.775 & 13.368 of PENTAPHYLLOIDES70.DȽликозиɞ220240260280300320340360380N"6468*DAD1, 13.501 (9.3 mAU, - ) Ref=13.368 & 13.715 of PENTAPHYLLOIDES70.DȽликозиɞ220240260280300320340360380N"620304050*DAD1, 15.655 (51.2 mAU, - ) Ref=15.508 & 15.775 of PENTAPHYLLOIDES70.DЭллɚɝоɜɚякиɫлоɬɚ220240260280300320340360380N"64681214*DAD1, 15.941 (12.8 mAU, - ) Ref=15.775 & 16.108 of PENTAPHYLLOIDES70.DȽликозиɞ220240260280300320340360380N"61234*DAD1, 16.635 (4.7 mAU, - ) Ref=16.515 & 16.708 of PENTAPHYLLOIDES70.DȽликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"620304050*DAD1, 16.855 (49.5 mAU, - ) Ref=16.708 & 17.175 of PENTAPHYLLOIDES70.DȽликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"6203040506070*DAD1, 18.121 (68.2 mAU, - ) Ref=17.835 & 18.488 of PENTAPHYLLOIDES70.D? 18,119Ƚликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"62345*DAD1, 18.635 (5.4 mAU, - ) Ref=18.501 & 18.768 of PENTAPHYLLOIDES70.DȽликозиɞкɜɟɪцɟɬинɚ220240260280300320340360380N"612345*DAD1, 19.068 (5.3 mAU, - ) Ref=18.921 & 19.235 of PENTAPHYLLOIDES70.DȽликозиɞкɟмпɮɟɪолɚ220240260280300320340360380N"60.51.52.5*DAD1, 19.361 (2.7 mAU, - ) Ref=19.235 & 19.588 of PENTAPHYLLOIDES70.DȽликозиɞкɟмпɮɟɪолɚ220240260280300320340360380N"681214161820*DAD1, 23.855 (15.8 mAU, - ) Ref=23.601 & 24.408 of PENTAPHYLLOIDES70.DКɜɟɪцɟɬин220240260280300320340360380N"651520*DAD1, 25.115 (23.5 mAU, - ) Ref=24.955 & 25.488 of PENTAPHYLLOIDES70.D? 25,116Ⱦɟпɫиɞɮлɚɜоноиɞɚокɫикоɪичнойкиɫлоɬы220240260280300320340360380N"623456789*DAD1, 27.808 (8.4 mAU, - ) Ref=27.668 & 27.948 of PENTAPHYLLOIDES70.DКɟмпɮɟɪолɭɞɟɪжиɜɚниякомпонɟнɬɚɯɪомɚɬоɝɪɚммɟɊиɫɭнокɋоɫɬɚɜполиɮɟнольныɯɫоɟɞинɟний P. fruticosa L.ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɊиɫɭнокɪɚɫпɪɟɞɟлɟниɟполиɮɟнолоɜполиɮɟнольномкомплɟкɫɟ P. fruticosa L.КɚкпɪиɜɟɞённомɪиɫɭнкɟоɫноɜнɭюмɚɫɫɭполиɮɟнолоɜɬɪɚɜыP. fruticosa ɫоɫɬɚɜляюɬɝликозиɞыкɜɟɪцɟɬинɚкɚɬɟɯиныɯлоɪоɝɟноɜɚялоɬɚколичɟɫɬɜɚɯпɪиɫɭɬɫɬɜɭюɬɝликозикɟмпɮɟɪолɚɏɚɪɚкɬɟɪнымпɪизнɚкомпɪоɞɭкɬоɜкиɫлоɬноɝоɝиɞɪолизɚполиɮɟнолоɜɬɪɚɜыP. fruticosa яɜилоɫьчɬояɪкоокɪɚɫкɭчɬоɭкɚзыɜɚɟɬпɪиɫɭɬɫɬɜиɟɚнɬоциɚноɜȽиɞɪолизɚɬɞɚлɟɟɯɪомɚɬоɝɪɚɮиɪоɜɚлиɪɟзɭльɬɚɬыɯɪомɚɬоɝɪɚɮиɪоɜɚнияпɪиɜɟɞɟныɪиɫɭнкɚɯɊиɫɭнокɏɪомɚɬоɝɪɚммɚполиɮɟнолоɜ P. fruticosa ɝиɞɪолизɚɞɟɬɟкцияɞиоɞноPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Ɋиɫɭнокɏɪомɚɬоɝɪɚммɚполиɮɟнолоɜ P. fruticosa ɝиɞɪолизɚɞɟɬɟкцияɞиоɞноɊɟзɭльɬɚɬыɪɟзɭльɬɚɬоɜкиɫлоɬноɝоɝиɞɪолизɚполиɮɟнолоɜɬɪɚɜыP. fruticosa пɪиɜɟɞɟныɪиɫɭнкɟ225250275300325350375N"6203040506070*DAD1, 24.152 (72.3 mAU, - ) Ref=23.852 & 24.938 of PENTAPHYLLOIDESGIDR.DКɜɟɪцɟɬин225250275300325350375N"651520*DAD1, 28.018 (24.0 mAU, - ) Ref=27.712 & 28.645 of PENTAPHYLLOIDESGIDR.DКɟмпɮɟɪол225250275300325350375N"60.51.52.53.5*DAD1, 30.585 (4.3 mAU, - ) Ref=30.432 & 30.825 of PENTAPHYLLOIDESGIDR.DtR ? 30,585225250275300325350375N"6406080100*DAD1, 16.005 (114 mAU, - ) Ref=15.812 & 16.572 of PENTAPHYLLOIDESGIDR.DКиɫлоɬɚэллɚɝоɜɚя220240260280300320340360380N"65152025*DAD1, 10.932 (25.9 mAU, - ) Ref=10.658 & 11.138 of PENTAPHYLLOIDESGIDR.DКиɫлоɬɚɯлоɪоɝɟноɜɚя250300350400450500550N"6-2.52.57.51012.51517.5*DAD1, 14.638 (21.6 mAU, - ) Ref=14.458 & 15.145 of PENTAPHYLLOIDESGIDR.DɊиɫɭнокɋоɫɬɚɜпɪоɞɭкɬоɜɝиɞɪолизɚполиɮɟнолоɜ P. fruticosa L.ɌɚкимоɛɪɚзомпɪоɞɭкɬɚɯɝиɞɪолизɚɚɝликоныɮлɚɜонолоɜɫɭщɟɫɬɜɟннымпɪɟоɛлɚɞɚкɜɟɪцɟɬинɚɌɚкжɟиɞɟнɬиɮициɪоɜɚнчɬоɫлɭжиɬпоɞɬɜɟɪжɞɟниɟмнɚличияполиɮɟнолɚɯиɫɫлɟɞɭɟмоɝопɪоɚнɬоциɚниɞиноɜɯоɞɟнɚɫɬоящɟɝоиɫɫлɟɞоɜɚнияɭɫɬɚноɜлɟночɬокɭɪильɫкийɫоɞɟɪжиɬɮлɚɜоноиɞыпɪɟɞɫɬɚɜɝликозиɞɚмиɬɪёɯɚɝликоноɜ ? кɜɟɪцɟɬинɚкɟмпɮɟɪолɚпɪɟоɛлɚɞɚниɟмпɟɪɜоɝоПɪиɫɭɬɫɬɜиɟɮлɚɜоноиɞоɜоɛъɟкɬɟкɚкоɛɭɫлоɜлиɜɚɟɬмножɟɫɬɜоɮɚɪмɚколоɝичɟɫкиɯэɮɮɟкɬоɜкɚпилляɪоɭкɪɟпляɚнɬиокɫиɞɚнɬныйпɪоɬиɜоопɭɯолɟɜыйɪɟзɭльɬɚɬɟɯɪомɚɬоɝɪɚɮиɪоɜɚнияпɪоɞɭкɬоɜкиɫлоɬноɝоɝиɞɪолизɚполиɮɟнолоɜиɫɫлɟɞɭɟмоɝооɛъɟкɬɚчɬоɫоɞɟɪжиɬɫяɛольшоɟколичɟɫɬɜопɪоɚнɬоциɚниɞиноɜɝɪɭппыɫоɟɞиɯɚɪɚкɬɟɪизɭющиɯɫяɜыɪɚжɟннымиɚнɬиокɫиɫɜойɫɬɜɚмиЭɬонɟɫомнɟнноопɪɟɞɟляɟɬпɟɪɫпɟкɬиɜноɫɬькɚкиɫɬочникɚэɬойɝɪɭппыɜɟщɟɫɬɜКɪомɟɬоɝопɪоɞɭкɬɚɯɝиɞɪолиɭɫɬɚноɜлɟнопɪиɫɭɬɫɬɜиɟэллɚɝоɜойкиɫлоɬычɬоɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyɭкɚзыɜɚɟɬпɪиɪоɞɭɞɭɛильныɯɜɟщɟɫɬɜэллɚɝоɬɚнниныЭллɚɝоɬɚнниныяɜляюɬɫяизɜɟɫɬпɪоɬиɜоɜоɫпɚлиɬɟльнымикɪоɜооɫɬɚнɚɜлиɜɚпɪоɬиɜоɬокɫичɟɫкимиɚɝɟнɬɚмиɋлɟɞоɜɚɞɟйɫɬɜɭющимнɚчɚломɬɪɚɜыP. fruticosa яɜляюɬɫяɬɪиклɚɫɫɚɫоɟɞинɟний ? ɮлɚɜоноиɞыɚнɬоциɚниɞиныэллɚɝоɬɚнниныНɚличиɟɬɚкоɝоɫпɟкɬɪɚполиɮɟнольныɯкомпонɟнɬоɜпозɜоляɟɬɭɬɜɟɪжɞɚɬьчɬоизɭчɟнныйоɛъɟкɬпɪɟɞɫɬɚɜляɟɬɫяпɟɪɫпɟкɬиɜнымиɫпользоɜɚниямɟɞицинɫкойпɪɚкɬикɟЭɬоɜозможноɫɬьɪɟкомɟнɞоɜɚɬьɞɚльнɟйшиɯɮɚɪмɚколоɝичɟɫкиɯиɫпыɬɚнийɪɚзɪɚɛоɬкилɟкɚɪɫɬɜɟнныɯпɪɟпɚɪɚɬоɜоɫноɜɟɟɝополиɮɟнольноɝокомплɟкɫɚЗȺКЛЮЧȿНИȿɪɟзɭльɬɚɬɟпɪоɜɟɞённыɯиɫɫлɟɞоɜɚнийɭɫɬɚноɜлɟночɬоɬɪɚɜɚP. fruticosa ɫоɞɟɪжиɬоколо 18 ɫоɟɞинɟнийполиɮɟнольнойɪоɞыпɪɟɞɫɬɚɜлɟнныɟɝликозиɞɚмиɚɝликонɚмиɮлɚɜонолоɜпɪоɚнɬоциɚниɞинɚмиокɫикоɪичнымилоɬɚмиэллɚɝоɬɚннинɚмиэɬомоколо 46% ɜɫɟйɫɭммыполиɮɟнолоɜɫоɫɬɚɜляюɬɮлɚɜоноиɞы, 24% ? пɪоɚнɬоциɚниɞиныокɫикоɪичныɟкиɫлоɬы, 7% эллɚɝоɬɚнниныПолɭчɟнныɟɪɟзɭльɬɚɬыпозɜоляюɬɭɬɜɟɪжɞɚɬьчɬоɫɬɚнɞɚɪɬизɚциюиɫɫлɟɞɭɟмоɝооɛъɟкможнопɪоɜоɞиɬьɫɭммɟполиɮɟнольныɯɫоɟɞипɟɪɟɫчёɬɟкɜɟɪцɟɬинкɚкɞоминиɪɭющɟɝокомпонɟнɬɚɫɭммыполиɮɟнолоɜпоɫлɟпɪɟɞɜɚɪиɬɟльноɝоɝиɞɪолизɚпɪоɚнɬоциɚниɞины L. L.) ? Kuril tea - is a plant from the Rosaceae family. The plant used to be referred to as the [1]. This is a popular plant in folk medicine in China, Tibet, Mongolia. Its owers owers diseases of the gastrointestinal tract, as a means to regu-late metabolism, menstruation. In Mongolian folk medi-cine it is used for diarrhea as a hemostatic [4, 5, 6]. This ammatory, antitumor and antiult-serogennye [4, 7, 8, 9, 10, 11, 12]. The current research cant antioxidant capacity in vitro and protective effect against Escherichia coli at Oxidative produced by phenolic components P. fruticosa L., [13, 14]. A comparative phytochemical study of three revealed the presence of hyperoside, catechin, caffeic and ferulic acid, rutin and ellagic acid in all of these objects. All of these species ex- and and A signi cant increase in the antioxidant activity of P. fruticosa fruticosa Chemical and pharmacological studies have shown that polyphenols of several classes, namely ? avonoids, P. fruticosaL. [17]. However, the content of pharmacologically sig- cant components in the plant composition can vary the growing region. This factor is re ected in the amount of ected in the amount of 23]. Accordingly, the evaluation of the qualitative com-P. fruticosa of this study P. fruticosa L. for standardizing its raw materials. The c direction direction MATERIALS AND METHODS. P. fruticosa L. herb collected in the Altai Territory was taken. The polyphenol complex from P. fruticosa L. herb was extracted with 70% ethyl alcohol at the ratio of 1:50 of raw materials to the extractant. The Agilent Technologies 1200 InAscentisexpress cation water and ethyl alcohol (according to GOST R 51652) were er. cient R and the asymmetry of the cient - was should be not more than - 1.5, and the asymmetry cient TTThe polyphenol complex was chromatographed under the following conditions:the mobile phase speed was 0,5 ml/min.;the column thermostat temperature was +35оɋ;the sample volume was 1 μl.The gradient elution was carried out under the conditions indicated in Table 1.Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Table 1 ? Conditions for the gradient elution of P. fruticosus L polyphenols Time, minA,%B,%09010108020207030305050 401090 avonols at 355 nm, hydroxycinnamic ac-avonols at 355 nm, hydroxycinnamic ac-The component composition was identi? ed by the avonoids aglucones, the acid hydrolysis of P. fruticosa polyphenols was carried out. The hydrolysis was 60 minutes. The resulting hydrolysate was ltered and RESULTS AND DISCUSSION. of the separation of alcohol extract from P. fruticosa L. herb is shown in Figure 1.Figure 1 ? Chromatograms of alcohol extract from herb P. fruticosa L.To assess the effectiveness of the separation of results of which are presented in Table 2.ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyTable 2 ? Results of calculations of ef ciency criteria of the chromatographic system for determination of P. fruticosa L. polyphenols 3.40521731.320.880.17224.82280222.880.690.12676.94682994.040.690.185.938132051.350.70.121713.1082770713.070.620.185313.502675851.510.790.122215.657672254.370.810.142215.940415771.091.120.184016.6351710312.921.380.094716.852921621.150.710.130718.119561414.791.210.1918.6381447082.061.020.115319.0651274352.080.910.125719.3641168801.360.740.133323.85211656517.710.640.164425.1162537915.690.740.117327.8053334973.080.920.1133where t is the component retention time, N is the number of theoretical plates, R is the peak separation factor, TBased on the results obtained in Table 2, the ef t into the referenced values proposed by the European Pharmacopoeia. Therefore, this chromato-graphic system can be considered effective. Decoding P. fruticosa ected in Figure 2. As follows from P. fruticosa L. herb is represented by catechines, avonoids, quercetin gly-P. fruticosa L. is shown in Figure 3.Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150220240260280300320340360380N"6406080100*DAD1, 3.408 (119 mAU, - ) Ref=3.188 & 3.715 of PENTAPHYLLOIDES70.DR*?3,405 Proanthocyanidin1220240260280300320340360380N"6100150200*DAD1, 4.821 (234 mAU, - ) Ref=4.555 & 5.321 of PENTAPHYLLOIDES70.DR ? 4,822Proanthocyanidin2220240260280300320340360380N"624681214*DAD1, 6.948 (14.9 mAU, - ) Ref=6.815 & 7.188 of PENTAPHYLLOIDES70.DR ? 6,946Ellagotannin220240260280300320340360380N"620304050607080*DAD1, 5.941 (83.6 mAU, - ) Ref=5.708 & 6.401 of PENTAPHYLLOIDES70.DR ? 5,938 Chlorogenic acid240260280300320340360380N"624681214*DAD1, 11.650 (14.3 mAU, - ) Ref=11.343 & 12.603 of PENTAPHYLLOIDES.D? 11,683220240260280300320340360380N"6246812*DAD1, 13.108 (12.0 mAU, - ) Ref=12.775 & 13.368 of PENTAPHYLLOIDES70.DR ? 13,108Glycoside oframnetine220240260280300320340360380N"6468*DAD1, 13.501 (9.3 mAU, - ) Ref=13.368 & 13.715 of PENTAPHYLLOIDES70.DR ? 13,502Glycoside of ramnetine220240260280300320340360380N"620304050*DAD1, 15.655 (51.2 mAU, - ) Ref=15.508 & 15.775 of PENTAPHYLLOIDES70.DR ? 15,657Ellagic acid220240260280300320340360380N"64681214*DAD1, 15.941 (12.8 mAU, - ) Ref=15.775 & 16.108 of PENTAPHYLLOIDES70.DR ? 15,940Glycoside oframnetine220240260280300320340360380N"61234*DAD1, 16.635 (4.7 mAU, - ) Ref=16.515 & 16.708 of PENTAPHYLLOIDES70.DtR ? 16,635Glycoside of quercetin220240260280300320340360380N"620304050*DAD1, 16.855 (49.5 mAU, - ) Ref=16.708 & 17.175 of PENTAPHYLLOIDES70.DR ? 16,852Glycoside of quercetin220240260280300320340360380N"6203040506070*DAD1, 18.121 (68.2 mAU, - ) Ref=17.835 & 18.488 of PENTAPHYLLOIDES70.D? 18,119220240260280300320340360380N"62345*DAD1, 18.635 (5.4 mAU, - ) Ref=18.501 & 18.768 of PENTAPHYLLOIDES70.DtR ? 18,638Glycoside of quercetin220240260280300320340360380N"612345*DAD1, 19.068 (5.3 mAU, - ) Ref=18.921 & 19.235 of PENTAPHYLLOIDES70.DtR ? 19,065Glycoside of kaempferol220240260280300320340360380N"60.51.52.5*DAD1, 19.361 (2.7 mAU, - ) Ref=19.235 & 19.588 of PENTAPHYLLOIDES70.DR ? 19,364Glycoside of kaempferol220240260280300320340360380N"681214161820*DAD1, 23.855 (15.8 mAU, - ) Ref=23.601 & 24.408 of PENTAPHYLLOIDES70.DR ? 23,852Quercetin220240260280300320340360380N"651520*DAD1, 25.115 (23.5 mAU, - ) Ref=24.955 & 25.488 of PENTAPHYLLOIDES70.D? 25,116 avonoid and 220240260280300320340360380N"623456789*DAD1, 27.808 (8.4 mAU, - ) Ref=27.668 & 27.948 of PENTAPHYLLOIDES70.Dis component retention time on the chromatogramFigure 2 ? Composition of P. fruticosa L polyphenolic compounds ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyFigure 3 ? Percentage distribution of polyphenols in the P. fruticosa L polyphenol complex gure, the main mass of P. fruticosaA characteristic feature of the products of acid P. fruticosa L. polyphenols was that they had a bright red color, indicating the presence of anthocyanins. The hydrolyzate was further Figure 4 ? Chromatogram of P. fruticosa L. polyphenols after acid hydrolysisPharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Figure 5 ? Chromatogram of P. fruticosa L. polyphenols after acid hydrolysis225250275300325350375N"6203040506070*DAD1, 24.152 (72.3 mAU, - ) Ref=23.852 & 24.938 of PENTAPHYLLOIDESGIDR.DR ? 24,152Quercetin225250275300325350375N"651520*DAD1, 28.018 (24.0 mAU, - ) Ref=27.712 & 28.645 of PENTAPHYLLOIDESGIDR.DR ? 28,018Kaempferol225250275300325350375N"60.51.52.53.5*DAD1, 30.585 (4.3 mAU, - ) Ref=30.432 & 30.825 of PENTAPHYLLOIDESGIDR.DtR ? 30,585Ramnetine225250275300325350375N"6406080100*DAD1, 16.005 (114 mAU, - ) Ref=15.812 & 16.572 of PENTAPHYLLOIDESGIDR.DR ? 16,007Ellagic acid220240260280300320340360380N"65152025*DAD1, 10.932 (25.9 mAU, - ) Ref=10.658 & 11.138 of PENTAPHYLLOIDESGIDR.DR ? 10,932Chlorogenic acid250300350400450500550N"6-2.52.57.51012.51517.5*DAD1, 14.638 (21.6 mAU, - ) Ref=14.458 & 15.145 of PENTAPHYLLOIDESGIDR.DFigure 6 ? Composition of products of hydrolysis of P. fruticosa L. polyphenols avonols with a signi cant ed, which rms the presence of proanthocyanidins in the polyphenols of the plant under study.In the course of this study, it has been established avonoids represented by ramnetin, with predominance of quercetin. The presence avonoids in the object, as a rule, causes a variety of diverse pharmacological effects, including capillary-strengthening, antioxidant, antitumor.hydrolysis of the investigated object?s polyphenols, it was found out that the plant contains a large number characterized by a pronounced antioxidant effect. This, of course, determines the prospects of the plant as a source of this valuable group of substances. In addition, the presence of ellagic acid has been established in the products of hydrolysis, which indicates the nature of tannins of the plant ? ellagatannins. Elvolatannins are ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, Botany ammatory, hemostatic, antitoxic agents. Consequently, the principle of Kuril tea (P. fruticosa L.) is avonoids, proanthocyanidins and elligotannins. The presence of such a spectrum of valuable polyphenolic components in the plant makes it possible to assert that the studied object seems promising in medical practice. Therefore, it can be recommended for further pharmacological testing and for the development of Kuril tea (P. fruticosa L.) avonols, proanthocyanidins, oxycinnamic acids, ellagatannins. About 46% of the avonoids, 24% are are ellagatannins. The results obtained make it possible ȻиɛлиоɝɪɚɮичɟɫкийȾикоɪɚɫɬɭщиɟполɟзныɟɊоɫɫии / ɪɟɞȻɭɞɚнцɟɜЛɟɫиоɜɫкɚяИзɞɋПɏɎȺЛɚɜɪɟноɜɚПолнɚяэнциклопɟɞиялɟкɚɪɫɬɜɟнныɯОЛМȺɋоколоɜɎиɬоɬɟɪɚпияɮиɬоɮɚɪмɚколоɝияɊɭкоɜоɞɫɬɜоɜɪɚчɟйМɟɞицинɫкоɟинɮоɪмɚционноɟɚɝɟнɬɫɬɜоG?rb?z I., ?zkan A.M., Yesilada E., Kutsal O. Anti-ulcerogenic activity of some plants used in folk medicine of Pinarbasi (Kayseri, Turkey) // Journal of ethnopharmacology. 2005. No. 101. Feng Y., Wu Z.H., Zhou X., Zhou Z., Fan W. Knowledge discovery in traditional Chinese medicine: state of the art and perspectives // Artif. intell. med. 2006. No. 38. Liu Y.H., Sun Y.L. China traditional Chinese medicine patent database // World ȺɪьяɟɜɚȺжɭноɜɚНиколɚɟɜэкɫɬɪɚкɬɚпоɛɟɝоɜ Pentaphylloides fruticosa (L.) ɬɟчɟниɟэкɫпɟɪимɟнɬɚльноɝоɫɚɯɚɪноɝоɞиɚɛɟɬɚɪɟɫɭɪɫыȿɜɫɬɪопоɜȻɭɪоɜɚȽɪɟк O.P. полиɮɟнольноɝокомплɟкɫɚэкɫɬɪɚɝиɪоɜɚнноɝопяɬилиɫɬникɚкɭɫɬɚɪникоɜоɝо (Penthaphylloides fruticosa (L.) пɪоɮилɚкɬикиКокɫɚкиɪɭɫнойȻюллɟɬɟньɫиɛиɪɫкоймɟɞициныTomczyk M., Leszczyska K., Jakoniuk P. Antimicrobial activity of Potentilla species / M. Tomczyk, K. ska, P. Jakoniuk // Fitoterapia. 2008. Vol. 79. No. 7. Miliauskas G., van Beek T.A., Venskutonis P.R., Linssen J.P., Waard P., Sudholter E.JR. Antioxidant activity of Wang L.-Y., Kou Y.-X., Wu G.-L., Wang Y.-J. Development and characterization of novel microsatellite cation in its sister species - Tomczyk M., Paduch R., Wiater A., Pleszczyska M., Kandefer-Szersze M., Szczodrak J. The in uence of aqueous extracts of selected Potentilla species on normal human colon cells // Actapoloniaepharmaceutica. Drug Miliauskas G., Mulder E., Linssen J.PH, Houben J.H., van Beek T.A., Venskutonis P.R. Evaluation of antioxidative Luo Z., Wang S., Wang D. Phenolic pro les and antioxidant capacities of crude extracts and subsequent fractions Wang S., Wang D., Pu W., Li D. Phytochemical pro les, antioxidant and antimicrobial activities of three Potentilla Liu Z., Luo J.C., Wang D. Synergistic effects of Potentilla fruticose L. leaves combined with green tea polyphenols in a variety of oxidation systems // Journal of food science. No. 81. P. 1091?1101.Chen C., Zhang X.W., Luo Z.M. Measurement of quercetin and kaempferol contents in Potentilla fruticosa from different regions by HPLC method // Chinese journal of analysis laboratory. 2009. ɏɪɚмоɜɚȾинɚмикɚɫоɞɟɪжɚнияɮлɚɜонолоɜнɚɞзɟмныɯ Pentaphylloides fruticosa (L.) O. экоɬипоɜНоɜоɫиɛиɪɫкɟɪɟɫɭɪɫыɏɪɚмоɜɚɋоɫɬɚɜɫоɞɟɪжɚниɟɮлɚɜоноиɞоɜ Pentaphylloides fruticosa пɪиɪоɞɟкɭльɬɭɪɟ // ɪɚɫɬиɬɟльноɝоɏɪɚмоɜɚОɫоɛɟнноɫɬинɚкоплɟнияɮɟнольныɯɫоɟɞинɟний Potentilla fructose (Rosaceae) ɬɟчɟнииɫɭɬокɪɚɫɬиɬɟльноɝоBai D.Y., Ma M.C., Zhang Z.H. Analysis of leaf ingredient in wild Potentilla fruticosa L. of different elevation // Pharmacy & Pharmacology V. 6 N 2, 2018DOI:10.19163/2307-9266-2018-6-2-135-150Li H.C., Sun H.Z., Hu X. Analysis on total avonoid in leaves of Potentilla fruticosa in different environment and Tomczyk M., Pleszczynska M., Wiater A. Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity // Molecules. 2010. Vol. 15. No. 7. 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Chinese journal of analysis laboratory. 2009;28:54?6.Khramova EP. Dinamika soderzhaniya avonolov v nadzemnykh organakh Pentaphylloides fruticosa (L.) O. Schwarz. razlichnykh ekotipov, vyrashchennykh v Novosibirske [Dynamics of the content of avonols in the aerial organs Pentaphylloides fruticosa (L.) O. Schwarz. different ecotypes grown in Novosibirsk]. Rastitelnye resursy. 1999;35(4):31?8. Russian.Khramova EP. Sostav i soderzhaniye avonoidov Pentaphylloides fruticosa v prirode i kul?ture [Composition avonoids Pentaphylloides fruticosa in nature and culture]. Chemistry of plant raw materials. ɎɚɪмɚцияɮɚɪмɚколоɝияɎɚɪмɚкоɝнозияɛоɬɚникɚPharmacognosy, BotanyKhramova EP. Osobennosti nakopleniya fenol?nykh soyedineniy v rasteniyakh Potentilla fructose (Rosaceae) Bai DY, Ma MC, Zhang ZH. Analysis of leaf ingredient in wild L. of different elevation avonoid in leaves of in different environment Tomczyk M, Pleszczynska M, Wiater A. Variation in total polyphenolics contents of aerial parts of Potentilla species and their anticariogenic activity. Molecules. 2010;15(7):4639?4651.Pisarev DI, Novikov OO, Sevruk IA, Malutina AU, Zinchenko AA, Guriev IV. Issledovaniye Ocimum basilicum . Issledovaniye Ocimum basilicum Ocimum basilicum L. within the framework of the scienti? c direction ?Pharmaceutical remake?]. Nauchnyy rezul?tat. Setevoy nauch.-prakt. zhurn. Ser. Meditsina. Farmatsiya. 2016;2(2):54?61. Russian.bourg, France: 2727.Markham KR, Bloor SJ. Analysis and identi cation of avonoids in practice. Flavonoids in health and disease. . New York; 1998. 1-34. (Antioxidants in health and disease). Con ict of interest ict of interest.Malyutina Anastasia Yuryevna ? PhD (Pharmacy)assistant professor of the Department of Pharmaceutical cational Establishment of Higher Professional Education of Belgorod State University, Ministry of Education and Science of the Russian Federation. Research interests: y, pharmacognosy. E-mail: ma-Pravlotskaya Alina Sergeevna ? post-graduate stu-lishment of Higher Professional Education of Belgorod State University, Ministry of Education and Science of Russia. Research interests: pharmaceutical chemistry, pharmacognosy. E-mail: 1163773@bsu.edu.ru ORCID: ? PhD (Pharmacy), Profes-sor, Head of the Department of Pharmaceutical Chem-tional Establishment of Higher Professional Education of Belgorod State University, Ministry of Education and Science of Russia. Research interests: pharmaceutical chemistry, pharmacognosy. PhD (Pharmacy), Associ-ate Professor, Professor of the Department of Pharmaceuti-ucational Establishment of Higher Professional Education of Belgorod State University, Ministry of Education and Sci-Research interests: phar-maceutical chemistry, pharmacognosy. E-mail: pisarev@Sent back for revision: 03.04.2018КонɮликɬинɬɟɪɟɫоɜȺɜɬоɪызɚяɜляюɬоɬɫɭɬɫɬɜииконɮликɬɚинɬɟɪɟɫоɜМɚлюɬинɚ ? кɚнɞиɞɚɬɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкɞоцɟнɬкɚɮɟɞɪыɮɚɪмɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииОɛлɚɫɬьнɚɭчныɯɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозия, ORCID: 0000-0001-6170-2151.Пɪɚɜлоцкɚяɋɟɪɝɟɟɜнɚ ? ɮɟɞɪыɮɚɪмɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫиилɚɫɬьнɚɭчныɯинɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозияE-mail: 1163773@bsu.edu.ru, ORCID: Ноɜикоɜ ? ɞокɬоɪɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкпɪоɮɟɫɫоɪзɚɜɟɞɭющийкɚɮɟɞɪоймɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииОɛлɚɫɬьнɚɭчинɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозияПиɫɚɪɟɜИɜɚноɜич ? ɞокɬоɪɮɚɪмɚцɟɜɬичɟɫкиɯнɚɭкɞоцɟнɬпɪоɮɟɫɫоɪкɚɮɟɞɪымɚцɟɜɬичɟɫкойɮɚɪмɚкоɝнозииɎȽȺОɍȻɟлȽɍМиноɛɪнɚɭкиɊоɫɫииОɛлɚɫɬьнɚɭчинɬɟɪɟɫоɜɮɚɪмɚцɟɜɬичɟɫкɚяɮɚɪмɚкоɝнозия. E-mail: pisarev@bsu.edu.ru, ПоɫɬɭпилɚɪɟɞɚкциюОɬпɪɚɜлɟнɚɞоɪɚɛоɬкɭПɪиняɬɚпɟчɚɬи

About the authors

A. Yu. Malyutina

Belgorod National State Research University of the Russian Federation

Email: malyutina_a@bsu.edu.ru

A. V. Pravlotskaya

Belgorod National State Research University of the Russian Federation

Email: 1163773@bsu.edu.ru

O. O. Novikov

Belgorod National State Research University of the Russian Federation

Email: novikov@bsu.edu.ru

D. I. Pisarev

Belgorod National State Research University of the Russian Federation

Email: pisarev@bsu.edu.ru

References

Supplementary files