FEATURES OF THE MICROBIOTA OF MATURE AND ELDERLY AGE IN NORMAL AND CHRONIC RHINOSINUSITIS


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Abstract

Aging is a natural process that is associated with morpho-functional rearrangement. Objective: to study the microbiota in a comparative aspect in adult patients with CRS aged 60-95 years and 45-59 years inclusive.

Materials and methods. The work was performed in GBUZ VOKB No. 1 on the basis of the otorhinolaryngological adult department, laboratory studies and identification of microorganisms were performed in the bacteriological department of the CDL Clinic No. 1 of the Federal State Budgetary Educational Institution of the Russian Ministry of Health. All patients underwent an endoscopic examination of the nasal cavity with a smear from the middle nasal passage. After sampling, the material was delivered to the laboratory for all types of bacteriological examination and identification of microorganisms.

Results. During the bacteriological study, 6 genera and 12 species of microorganisms were isolated (relative frequency of isolation %), of which Staphylococcus spp. (78.45%) and Enterococcus spp. (16.45%) were the main representatives of the microbiota of the nasal cavity in patients 45-95 years old. Staphylococcus spp. represent the basis of the microbiotype in the sinonasal microbiome and the predominant genus in patients, regardless of the pathology of the nose and paranasal sinuses.

The bacterial species Staphylococcus aureus (45.48%), Staphylococcus haemolyticus (19.57%) and Enterococcus faecalis (9.99%) were the three dominant types in all age groups. However, in patients aged 60-95 years with CRS, in addition to the above groups of Staphylococcus spp. (66.67%), Enterococcus spp. (10.67%), representatives of the groups Pseudomonadales (6.01%), Candidiales (6.0%) were seeded. In patients aged 60-95 years with CRS, the microbial landscape of the nasal mucosa is represented by various types of Staphylococcus spp.

Conclusions. The microbiota in patients with CRS aged 60-95 years is very diverse compared to younger age and patients without inflammatory diseases of the nose and paranasal sinuses.

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About the authors

Natalia V. Tarasova

Volgograd State Medical University

Email: tarasova.nv@ruskmv.ru
ORCID iD: 0000-0003-1929-5155
SPIN-code: 7889-4220

MD, Dr. Sci. (Med.), professor

Russian Federation, Volgograd

Irina S. Stepanenko

Volgograd Medical University

Email: ymahkina@mail.ru
ORCID iD: 0000-0001-5793-438X
SPIN-code: 4826-6040
Scopus Author ID: 643365

Doctor of Medical Sciences , Professor

Russian Federation, Volgograd st. Square of Fallen Fighters, 1

Eleonora B. Belan

Volgograd State Medical University

Email: belan.eleonora@yandex.ru
ORCID iD: 0000-0003-2674-4289

Dr. Sci. (Med.), Professor, Head of the Department of Immunology and Allergology

Russian Federation, Volgograd

Maria Sokolova

Volgograd state medical university

Author for correspondence.
Email: mulia2585@yandex.ru
ORCID iD: 0009-0001-5503-2646

Аспирант кафедры оториноларингологии 

Russian Federation, Volgograd st. Square of Fallen Fighters, 1

Vyacheslav A. Kosov

Volgograd Medical University

Email: Slava.kosov.1999@bk.ru
SPIN-code: 6907-0278
Scopus Author ID: 1236738
Russian Federation, Volgograd st. Square of Fallen Fighters, 1

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