Features of the microbiota of adults and older people under normal and chronic rhinosinusitis conditions

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BACKGROUND: Aging is naturally associated with morphofunctional rearrangement.

AIM: This study aimed to examine and compare the microbiota of adult patients with chronic rhinosinusitis aged 60–95 and 45–59 years.

MATERIALS AND METHODS: The study was performed in Volgograd Regional Clinical Hospital No. 1 in an otorhinolaryngological adult department. Laboratory studies and microorganism identification were performed in the bacteriological department of the clinical diagnostic laboratory of Clinic No. 1 of Volgograd State Medical University. All patients underwent endoscopic examination of the nasal cavity with a smear from the middle nasal passage. After sampling, the material was delivered to the laboratory for all bacteriological examinations and microorganism identification.

RESULTS: During the bacteriological study, 6 genera and 12 species of microorganisms were isolated (relative frequency of isolation %), and Staphylococcus spp. (78.45%) and Enterococcus spp. (16.45%) were the main representatives of the microbiota in the nasal cavity of patients aged 45–95 years. Staphylococcus spp. represent the basis of the microbiotype in the sinonasal microbiome and the predominant genus in patients regardless of the pathologies of the nose and paranasal sinuses. Staphylococcus aureus (45.48%), Staphylococcus haemolyticus (19.57%), and Enterococcus faecalis (9.99%) were the three dominant types in all age groups. However, in patients aged 60–95 years with chronic rhinosinusitis, in addition to Staphylococcus spp. (66.67%) and Enterococcus spp. (10.67%), representatives of Pseudomonadales (6.01%) and Candidiales (6.0%) were also observed. In patients aged 60–95 years with chronic rhinosinusitis, the microbial landscape of the nasal mucosa was represented by various strains of Staphylococcus spp.

CONCLUSIONS: The microbiota in patients aged 60–95 years with chronic rhinosinusitis is very diverse compared with those in younger individuals and patients without inflammatory diseases of the nose and paranasal sinuses.

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作者简介

Natalia Tarasova

Volgograd State Medical University; Volgograd Regional Clinical Hospital No. 1

Email: tarasova-nv@mail.ru
ORCID iD: 0000-0003-1929-5155
SPIN 代码: 7889-4220

MD, Dr. Sci. (Med.), professor

俄罗斯联邦, Volgograd; Volgograd

Irina Stepanenko

Volgograd State Medical University

Email: ymahkina@mail.ru
ORCID iD: 0000-0001-5793-438X
SPIN 代码: 4826-6040
Scopus 作者 ID: 643365

Doctor of Medical Sciences, Professor

俄罗斯联邦, Volgograd

Eleonora Belan

Volgograd State Medical University

Email: belan.eleonora@yandex.ru
ORCID iD: 0000-0003-2674-4289

MD, Dr. Sci. (Medicine), Professor

俄罗斯联邦, Volgograd

Maria Sokolova

Volgograd state medical university; Volgograd Regional Clinical Hospital No. 1

Email: sokolova.zmv@yandex.ru
ORCID iD: 0009-0001-5503-2646

Assistant of the Department of Otorhinolaryngology

俄罗斯联邦, Volgograd; Volgograd

Vyacheslav Kosov

Volgograd State Medical University

编辑信件的主要联系方式.
Email: Slava.kosov.1999@bk.ru
SPIN 代码: 6907-0278
Scopus 作者 ID: 1236738

Postgraduate student

俄罗斯联邦, Volgograd

参考

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2. Fig. 1. Block diagram of the study design. CRS, chronic rhinosinusitis

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3. Рис. 2. Бактериальная колонизация слизистой оболочки полости носа у пациентов 45–95 лет (группы 1–3)

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4. Рис. 3. Бактериальная колонизация слизистой оболочки полости носа у пациентов 45–95 лет

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5. Fig. 5. Bacterial colonization of the nasal mucosa in patients aged 60–95 years with chronic rhinosinusitis (group 1)

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6. Fig. 6. Types of microorganisms identified during bacteriological examination in patients aged 45–59 years with chronic rhinosinusitis

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7. Fig. 7. Bacterial colonization of the nasal mucosa in patients aged 45–59 years with chronic rhinosinusitis

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