SEARCH FOR BIODEGRADABLE POLYMER MATERIAL FOR RECONSTRUCTION OF TYMPANIC MEMBRANE DEFECTS


Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Тек жазылушылар үшін

Аннотация

Biocompatible polymer matrices are extensively investigated as materials for the reconstruction of chronic tympanic perforations. In this study, bilayer polymer films were prepared by the casting method using chitosan solutions with molecular weights of 500 and 900 kDa (CS500 and CS900, respectively) and hyaluronic acid (HA) with a molecular weight of 1300 kDa. The samples were also treated at 100°C for 5 minutes (samples marked with t). Toxicity, biodegradation rate and biocompatibility of the material were evaluated in 20 Wistar rats weighing 220-240 g. The rats were observed on days 7, 14, 30 and 50 after subcutaneous implantation. No acute toxicity, septic or allergic inflammation, or scarring of surrounding tissues was observed during the post-implantation period. The biodegradation rate was found to decrease in the following order: CS500-HA ≥ CS900-HA > CS500-HA(t) > CS900_HA(t). The study demonstrates the effect of chitosan molecular weight and thermal treatment on the degradation rate and the process of polymer implant biodegradation as well as the type of reactive proliferation of connective tissue. These results support further preclinical research on the polymer film samples for the development of matrices for tympanoplasty tasks.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Maria Naumenko

Pavlov First Saint Petersburg State Medical University, 197022, Russian Federation, Saint Petersburg

Email: naumenkomyu@gmail.com
ORCID iD: 0009-0003-8053-6381

6th year student, Department of Otorhinolaryngology, Laboratory of Hearing and Speech.

Ресей, Санкт-Петербург, ул. Л. Толстого, д. 6-8;

Petr Snetkov

Center of Chemical Engineering, ITMO University.

Email: ppsnetkov@itmo.ru
ORCID iD: 0000-0001-9949-5709
SPIN-код: 2951-3791
Scopus Author ID: 57205168040

Candidate of Technical Sciences, Senior Researcher at the Institute of Advanced Data Transmission Systems

Ресей, 49 Kronverksky Ave., Saint Petersburg

Svetlana Morozkina

Laboratory for the Development of drug Delivery Systems of the Federal State Budgetary Institution "SPb NIIF" of the Ministry of Health of the Russian Federation

Email: morozkinasvetlana@gmail.com
ORCID iD: 0000-0003-0122-0251
SPIN-код: 3215-0328
Scopus Author ID: 6507035544
ResearcherId: M-1252-2013

Candidate of Chemical Sciences, Head of the Laboratory for the Development of Drug delivery systems

Ресей, Санкт-Петербург, Лиговский пр., д.2-4

Anna Bervinova

Pavlov First Saint Petersburg State Medical University, 197022, Russian Federation, Saint Petersburg

Email: anna.bervinova@mail.ru
ORCID iD: 0000-0002-2898-4916

Ph.D. in Medicine, an otorhinolaryngologist

Ресей, Saint Petersburg, L. Tolstoy Street 6-8.

Galina Yukina

Pavlov First Saint Petersburg State Medical University, 197022, Russian Federation, Saint Petersburg

Email: pipson@inbox.ru
ORCID iD: 0000-0001-8888-4135

Candidate of Biological Sciences, Associate Professor, Head of the Scientific Laboratory of Pathomorphology

Ресей, Saint Petersburg, L. Tolstoy Street 6-8.

Sergei Zhuravskii

Pavlov First Saint Petersburg State Medical University

Хат алмасуға жауапты Автор.
Email: s.jour@mail.ru
Scopus Author ID: 8244733500

MD, Senior Researcher at the Laboratory of Hearing and Speech

Ресей, Saint Petersburg, L. Tolstoy Street 6-8.

Әдебиет тізімі

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