Search for biodegradable polymer material for the reconstruction of tympanic membrane defects

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Дәйексөз келтіру

Толық мәтін

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Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

BACKGROUND: Biocompatible polymer matrices are extensively investigated as materials for the reconstruction of chronic tympanic perforations.

AIM: This study aimed to evaluate the general and local toxicity and biodegradation and biocompatibility mechanism of samples of two-layer polymer films based on chitosan (CS) and hyaluronic acid (HA).

MATERIALS AND METHODS: Bilayer polymer films were prepared by the casting method using CS solutions with molecular weights of 500 and 900 kDa (CS500 and CS900, respectively) and HA with a molecular weight of 1300 kDa. The samples were also treated at 100°C for 5 min (samples marked with t). The toxicity, biodegradation rate, and biocompatibility of the materials were evaluated in 20 Wistar rats weighing 220–240 g. The rats were observed on days 7, 14, 30, and 50 after subcutaneous implantation.

RESULTS: No acute toxicity, septic or allergic inflammation, or scarring of surrounding tissues was observed during the post-implantation period. The biodegradation rate decreased in the following order: CS500-HA (whitout t) ≥ CS900-HA (whitout t) > CS500-HA (t) > CS900_HA (t). The study demonstrated the effect of CS in different molecular weights and thermal treatment on the degradation rate and polymer implant biodegradation as well as the type of reactive proliferation of the connective tissue.

CONCLUSIONS: These results support further preclinical research on polymer film samples for the development of matrices for tympanoplasties.

Толық мәтін

Рұқсат жабық

Авторлар туралы

Maria Naumenko

Academician I.P. Pavlov First St. Petersburg State Medical University

Email: naumenkomyu@gmail.com
ORCID iD: 0009-0003-8053-6381
Ресей, Saint Petersburg

Petr Snetkov

ITMO University; Institute of Macromolecular Compounds; Saint Petersburg State Research Institute of Phthisiopulmonology

Email: ppsnetkov@itmo.ru
ORCID iD: 0000-0001-9949-5709
SPIN-код: 2951-3791
Scopus Author ID: 57205168040

Cand. Sci. (Technology)

Ресей, Saint Petersburg; Saint Petersburg; Saint Petersburg

Svetlana Morozkina

ITMO University; Institute of Macromolecular Compounds; Saint Petersburg State Research Institute of Phthisiopulmonology

Email: morozkina.svetlana@gmail.com
ORCID iD: 0000-0003-0122-0251
SPIN-код: 3215-0328
Scopus Author ID: 6507035544
ResearcherId: M-1252-2013

Cand. Sci. (Chemistry)

Ресей, Saint Petersburg; Saint Petersburg; Saint Petersburg

Anna Bervinova

Academician I.P. Pavlov First St. Petersburg State Medical University

Email: anna.bervinova@mail.ru
ORCID iD: 0000-0002-2898-4916

MD, Cand. Sci. (Medicine)

Ресей, Saint Petersburg

Galina Yukina

Academician I.P. Pavlov First St. Petersburg State Medical University

Email: pipson@inbox.ru
ORCID iD: 0000-0001-8888-4135

Cand. Sci. (Biology), Assistant Professor

Ресей, Saint Petersburg

Sergei Zhuravskii

Academician I.P. Pavlov First St. Petersburg State Medical University

Хат алмасуға жауапты Автор.
Email: s.jour@mail.ru
Scopus Author ID: 8244733500

MD, Dr. Sci. (Medicine)

Ресей, Saint Petersburg

Әдебиет тізімі

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2. Fig. 1. Dynamics of the changes in animal weights during the experiment. CS, chitosan; HA, hyaluronic acid

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3. Fig. 2. Implantation zone on postoperative day 3. Implantation of a polymer film from CS900_GA (without t). Reproduced from article [20] with permission from MDPI, 2023

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4. Fig. 3. Dynamics of implantation zone edema. CS, chitosan; HA, hyaluronic acid

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5. Fig. 4. Implantation site (a) and type of matrix in the swelling state (b) at the fixation stage by day 50 of observation. A sample of the CS900_HA (t) film. Macropreparations: (a) magnification ×2 and (b) magnification ×4. Reproduced from article [20] with the permission of MDPI, 2023

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6. Fig. 5. Samples of chitosan material with a molecular weight of 500 kDa and hyaluronic acid on day 50 of implantation. Staining by the Mallory method. Magnification ratio 200: (a), without heat treatment; matrix fragments (indicated by arrows), fibroblasts in the biodegradation zone; (b) with heat treatment; fragments of the matrix material (indicated by arrows)

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7. Fig. 6. Samples of chitosan materials with a molecular weight of 900 kDa and hyaluronic acid on day 50 of implantation. Decay zone of the matrix and the periimplantation capsule stained by the Mallory method. Magnification ratio, 100: (a) without heat treatment and (b) with heat treatment

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