Role of the c825t polymorphic locus of G-protein beta subunit (GNBp3) in patients with gastroesophageal reflux disease

Genetic factors play an important role in the development of various diseases of the gastrointestinal tract, including gastroesophageal reflux disease (GERD). Aim of the research was to study the prevalence of genetic polymorphism of the GNBB3 825C/Tgene in patients with GERD living in the Trans-Baikal Territory, depending on the clinical picture of the disease and the nature of refluxate. Material and methods. The study involved 139 people who identify themselves as Caucasians and have lived in the Trans-Baikal Territory for at least three generations time. The first group included 79 patients with GERD (30 male and 49 female patients, median age was 43 (33; 57) years), the second (control) group included 60 people (28 males and 32 females, median age - 44 (37; 54) years). Genotyping of the C825T polymorphic locus of the G-protein beta subunit (GNBB3) was performed by PCR methodic. Methods of nonparametric statistics were used, the differences were considered significant at p <0,05. Results. Both groups revealed the presence of three genotypes GNBB3 C825T (C/C, T/T, C/T). There were no differences in the prevalence of genotypes and alleles between patients with GERD and the control group. Patients who experience belching 1 or more times a week are more likely to have the GNBB3 825C/T genotype (OR=3,235; 95% CI: 0,838-12,488; p=0,052), while the prevalence of the T allele in these patients did not differ. In patients with heartburn occurring at least 1 time per week, there was a tendency towards the most frequent spread of this genotype (p=0,066). Conclusion. The prevalence of 825 C / T GNBB3 genotypes was comparable in patients with GERD and in the control group. The CNBB3 825 C / T genotype is associated with GERD with clinical manifestations of the disease at least 1 time per week.

gastroesophageal reflux disease, GNBB3 gene polymorphism, heartburn, regurgitation, impedance pH measurement