Clinical and diagnostic importance of intestinal microbiota in the development of non-specific ulcerative colitis
- Authors: Lagutina S.N.1, Chizhkov P.A.2, Zuikova A.A.1, Dobrynina I.S.1, Popov V.N.2
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Affiliations:
- N.N. Burdenko Voronezh State Medical University of the Ministry of Healthcare of Russia
- Voronezh State University
- Issue: Vol 9, No 7 (2023)
- Pages: 17-21
- Section: ORIGINAL STUDIES
- URL: https://journals.eco-vector.com/2412-4036/article/view/625418
- DOI: https://doi.org/10.18565/therapy.2023.7.17–21
- ID: 625418
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Abstract
Currently, inflammatory bowel diseases with an autoimmune type of lesion (ulcerative colitis, Crohn’s disease) occupy a leading position among gastrointestinal tract (GIT) pathologies. Changes in the generic composition of the intestinal microbiota may be an early diagnostic sign of autoimmune inflammation.
The aim: to assess the biodiversity of the gut microbiota in patients with ulcerative colitis (UC).
Material and methods. 16s-RNA genetic sequencing (the main method for studying intestinal microbiota) was carried out on 40 persons – 20 patients with UC of varying severity (main group) and 20 somatically healthy individuals (control group). In all patients, the diagnosis of UC was previously verified accordingly to the results of colonoscopy and targeted biopsy followed by histological examination. The severity of the course of UC was identified by Mayo index. In addition, laboratory diagnostics was carried out. It contained the estimation of indexes of general and biochemical (CRP) blood tests in both study groups. The average age of examined patients was 42±3,5 years.
Results. When assessing laboratory parameters, patients with UC showed leukocytosis (11,4±0,68 g/l), as well as an increase in CRP (9,6±1,5 mg/l) comparatively to the control group. When interpreting Mayo index, most patients had an average severity of the underlying disease. As a result of sequencing of the intestinal microbiota in the main group, comparatively to the control one, a significant decrease in representatives of the normal intestinal microflora – the main producers of short-chain fatty acids, and an increase in bacteria of the pathogenic cluster and sulfate-reducing bacteria were revealed.
Conclusion. A significant decrease in normal microflora and an increase in the pathogenic cluster of bacteria in patients with UC were found. The obtained data can form the basis for improving the diagnosis of this pathology.
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About the authors
Svetlana N. Lagutina
N.N. Burdenko Voronezh State Medical University of the Ministry of Healthcare of Russia
Author for correspondence.
Email: svlagutina97@mail.ru
ORCID iD: 0000-0003-3730-5265
resident of the Department of outpatient therapy
Russian Federation, 394036, Voronezh, 10 Studencheskaya Str.Pavel A. Chizhkov
Voronezh State University
Email: qooleer@yandex.ru
ORCID iD: 0000-0002-5626-0579
postgraduate student of the Department of genetics, cytology and bioengineering
Russian Federation, 394036, Voronezh, 10 Studencheskaya Sq.Anna A. Zuikova
N.N. Burdenko Voronezh State Medical University of the Ministry of Healthcare of Russia
Email: zuikova-therapia@vrngmu.ru
ORCID iD: 0000-0002-5378-4959
MD, professor, head of the Department of polyclinic therapy
Russian Federation, 394036, Voronezh, 10 Studencheskaya Str.Irina S. Dobrynina
N.N. Burdenko Voronezh State Medical University of the Ministry of Healthcare of Russia
Email: dobrynina@mail.ru
ORCID iD: 0000-0002-4849-0200
PhD in Medical Sciences, associate professor of the Department of polyclinic therapy
Russian Federation, 394036, Voronezh, 10 Studencheskaya Str.Vasily N. Popov
Voronezh State University
Email: pvn@vsuet.ru
ORCID iD: 0000-0003-1294-8686
MD, professor, head of the Department of genetics, cytology and bioengineering
Russian Federation, 394036, Voronezh, 10 Studencheskaya Sq.References
- Lin L., Zhang J. Role of intestinal microbiota and metabolites on gut homeostasis and human diseases. BMC Immunology. 2017; 18(1): 2. https://dx.doi.org/10.1186/s12865-016-0187-3.
- Derovs A., Laivacuma S., Krumina A. Targeting microbiota: What do we know about it at present? Med (Kaunas). 2019; 55(8): 459. https://dx.doi.org/10.3390/medicina55080459.
- Burakova I., Smirnova Y., Gryaznova M. et al. The effect of short-term consumption of lactic acid bacteria on the gut microbiota in obese people. Nutrients. 2022; 14(16): 3384. https://dx.doi.org/10.3390/nu14163384.
- Chattopadhyay I., Dhar R., Petusami K. et al. Exploring the role of the gut microbiome in colon cancer. Applied Biochemistry Biotechnol. 2021; 193(6): 1780–99. https://dx.doi.org/10.1007/s12010-021-03498-9.
- Loke Y.L., Chew M.T., Ngeow Y.F. et al. Colon carcinogenesis: The interplay between diet and gut microbiota. Front Cell Infect Microbiol. 2020; 10: 603086. https://dx.doi.org/10.3389/fcimb.2020.603086.
- Sanchez-Alcoholado L., Ramos-Molina B., Otero A. et al. The role of the gut microbiome in colorectal cancer development and therapy response. Cancers (Basel). 2020; 12(6): 1406. https://dx.doi.org/10.3390/cancers12061406.
- Sekizuka T., Ogasawara Y., Ohkusa T., Kuroda M. Characterization of Fusobacterium varium Fv113-g1 isolated from a patient with ulcerative colitis based on complete genome sequence and transcriptome analysis. PLoS One. 2017; 12(12): e0189319.
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