Study Of The Influence Of Modification Of The 2Н1Н Isotopic Composition Of The Medium On The Growth Of Biomass And Respiratory Activity Of The Bacterial Culture SHEWANELLA ONEIDENSIS MR-1

The work investigated the effect of modification of the 1Н/2Н isotopic composition of the medium on the growth of biomass and respiratory activity of the bacterial culture Shewanella oneidensis MR-1. An experimental study of the theory of isotope resonance by R.A. Zubarev was carried out. The growth of cultures in the incubation medium was assessed by measuring optical density using a Thermo Scientific Multiskan FC multiplate photometer. To determine the optical density, a Multiscan FC photometer for ELISA studies in microplates was used. The determination of the deuterium concentration in the resulting medium was carried out using a JEOL JNM-ECA 400MHz pulsed NMR spectrometer. It was found that groups with 2H contents of 150, 350 and 370 ppm demonstrated positive trends in both biomass growth and CO2 emissions (p < 0.05). At a 2H level of 200 ppm throughout the experiment, CO2 emissions were slightly less than in all control groups, with the exception of measurements after 14 hours. It has been established that a change in the natural ratio of isotopes in some structural components of living systems is accompanied by a modification of some mechanisms of biochemical reactions in biological objects, which is due to, for example, compartmentalization and, in general, this can lead to faster adaptation under the influence of various stress factors. The obtained results can be explained by the presence of a phenomenon known as “isotope shock” which can be realized through the formation of an isotope gradient that stimulates the work of the nonspecific defense system, leading to the accumulation of biologically active protective factors in the body.

isotope, protium, deuterium, isotope shock, biological systems, bacterial culture