Genetic diversity of barley accessions from Ethiopia for the powdery mildew resistance

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Abstract


Nine hundred and twenty five Ethiopian barley accessions were studied under laboratory experiments for juvenile resistance to North West population of powdery mildew casual agent Blumeria graminis (DC.) Golovin ex Speer f. sp. hordei Marchal. Based on phenotypic screening, the 27 accessions resistant to the pathogen were selected and 47 forms were heterogeneous for the studied character. The resistant and susceptible plants of the examined accessions were analyzed with the use of molecular markers developed for identification of the mlo11 allele. Fifteen accessions carrying the mlo11 allele which determines durable resistance of most modern barley varieties to powdery mildew were revealed. The accessions differed by the resistance expression that can be explained both by the manifestation of other resistance genes which are inadequate to the mlo11 and also by the presence of different mlo11 allelic variants in the genotypes of the selected accessions. The resistance to B. graminis of other 59 forms is controlled by the effective genes which are different from mlo11.


About the authors

R. A. Abdullaev

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Author for correspondence.
Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

candidate of biological sciences

T. V. Lebedeva

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

candidate of biological sciences

N. V. Alpatieva

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

candidate of biological sciences

O. V. Yakovleva

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

candidate of biological sciences

O. N. Kovaleva

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

candidate of biological sciences

E. E. Radchenko

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

doctor of biological sciences

I. N. Anisimova

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

doctor of biological sciences

B. A. Batasheva

Dagestan Experiment Station of VIR

Email: abdullaev.1988@list.ru

Russian Federation, Derbent, Derbentskiy rayon, s. Vavilovo

doctor of biological sciences

Yu. I. Karabitsina

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

E. B. Kuznetsova

Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources

Email: abdullaev.1988@list.ru

Russian Federation, St. Petersburg

References

  1. Вавилов Н.И. Происхождение и география культурных растений. – Л.: Наука, 1987. – 440с.
  2. Bonman J.M., Bockelman H.E., Jackson L.F., Steffenson B.J. Disease and insect resistance in cultivated barley accessions from the USDA National Small Grains Collection // Crop Sci. – 2005. – V. 45. – № 4. – P. 1271-1280.
  3. Коновалова Г.С., Соболева О.Н. Источники устойчивости ячменя из Юго-Восточной Азии к возбудителю ринхоспориоза (Rhynchosporium secalis) // Микология и фитопатология. – 2010. – Т. 44. – Вып. 3. – С. 248-254.
  4. Хохлова А.П. Устойчивость ячменя к пыльной головне и состав генов, контролирующих этот признак. Дис. … канд. с.-х. наук. – Л.: ВИР. – 1990. – 151 с.
  5. Weibull J., Walther U., Sato K., Habekuβ A., Kopahnke D., Proeseler G. Diversity in resistance to biotic stresses // Diversity in barley (Hordeum vulgare). Elsevier, 2003. – P. 143-178.
  6. Reinstädler A., Müller J., Jerzy H., Czembor J.H., Piffanelli P., Panstruga R. Novel induced mlo mutant alleles in combination with site-directed mutagenesis reveal functionally important domains in the heptahelical barley Mlo protein // BMC Plant Biology. – 2010. – V. 10. – Article 31. https://doi.org/10.1186/1471-2229-10-31.
  7. Iovieno P., Andolfo G., Schiavulli A., Catalano D., Ricciardi L., Frusciante L., Raffaella M., Pavan S. Structure, evolution and functional inference on the Mildew Locus O (MLO) gene family in three cultivated Cucurbitaceae spp. // BMC Genomics. – 2015. – V. 16. – Article 1112. https://doi.org/10.1186/s12864-015-2325-3.
  8. Вüschges R., Hollricher K., Panstruga R., Simons G, Wolter M., Frijters A., Van Daelen R., Van der Lee T., Diergaarde P., Groenendijk J., Töpsch S., Vos P., Salamini F., Schulze-Lefert P. The barley Mlo gene: a novel control element of plant pathogen resistance // Cell. – 1997. – V. 88. – № 5. – P. 695-705.
  9. Wolter M., Hollricher K., Salamini F., Schulze-Lefert P. The mlo resistance alleles to powdery mildew infection in barley trigger a developmentally controlled defense mimic phenotype // Mol. Gen. Genet. – 1993. – V. 239. – № 1-2. – P. 122-128. https://doi.org/10.1007/ BF00281610.
  10. Dreiseitl A. Frequency of powdery mildew resistances in spring barley cultivars in Czech variety trials // Plant Prot. Sci. – 2012. – V. 48. – № 1. – P. 17-20. https://doi.org/10.17221/11/2011-PPS.
  11. Piffanelli P., Ramsay L., Waugh R., Benabdelmouna A., D'Hont A., Hollricher K., Jørgensen J.H., Schulze-Lefert P., Panstruga R. A barley cultivation-associated polymorphism conveys resistance to powdery mildew // Nature. – 2004. – V. 430. – № 7002. – P. 887-891.
  12. Ge X., Deng W., Lee Z.Z., Lopez-Ruiz F.J., Schweizer P., Ellwood S.R. Tempered mlo broad-spectrum resistance to barley powdery mildew in an Ethiopian landrace // Scientific Reports. – 2016. – V.6. – Article 29558. doi: org/10.1038/srep29558.
  13. Алпатьева Н.В., Абдуллаев Р.А., Анисимова И.Н., Губарева Н.К., Ковалева О.Н., Радченко Е.Е. Устойчивые к мучнистой росе образцы местного ячменя из Эфиопии // Труды по прикл. бот., ген. и сел. – 2016. – Т. 177. – Вып. 4. – С. 70-78.
  14. Mains E.B., Dietz S.M. Physiologic forms of barley mildew Erysiphe graminis DC. // Phytopath. – 1930. – V. 20. – № 3. – P. 229-239.
  15. Дорохов Д.Б., Клоке Э. Быстрая и экономичная технология RAPD анализа растительных геномов // Генетика. – 1997. – Т. 33. – № 4. – С. 443-450.
  16. Анисимова И.Н., Алпатьева Н.В., Тимофеева Г.И. Скрининг генетических ресурсов растений с использованием ДНК-маркеров: основные принципы, выделение ДНК, постановка ПЦР, электрофорез в агарозном геле. Методические указания. – СПб.: ВИР, 2010. – 30 с.

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