The change in the functional status of native and cryopreserved spermatozoa of bulls under the influence of caffeine

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Abstract

The aim of the study is to study the effect of caffeine on the functional status of native and thawed bovine spermatozoa. Incubation of intact non-frozen bovine spermatozoa during 4 hours with caffeine increased the number of capacitated and acrosome-reactive cells, while the incubation in the presence of combination prolactin/ guanosine triphosphate did not affect the proportion of uncapacitated, capacitateded, and acrosome-reactive cells. Pre-incubation of spermatozoa and the subsequent freezing led to the increase in number of capacitated spermatozoa and acrosome-reactive cells in thawed samples, incubated with the caffeine, and there was no effect on functional status of spermatozoa incubated with prolactin and guanosine triphosphate. Thus, pre-incubation of spermatozoa before freezing with the caffeine altered their functional status after thawing (the decrease in the number of capacitated cells), while the combined action of prolactin and guanosine triphosphate did not affect indicator. 

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About the authors

I. V. Chistyakova

All-Russian research institute of genetics and breeding of farm animals –  the branch of Federal Research Center Livestock

Author for correspondence.
Email: itjerena7@gmail.com

postgraduate student

Russian Federation, Sankt-Peterburg-Pushkin, Moscovskoe sh., 55 a

T. I. Kuzmina

All-Russian research institute of genetics and breeding of farm animals –  the branch of Federal Research Center Livestock

Email: itjerena7@gmail.com

doctor of biological sciences

Russian Federation, Sankt-Peterburg-Pushkin, Moscovskoe sh., 55 a

V. Yu. Denisenko

All-Russian research institute of genetics and breeding of farm animals –  the branch of Federal Research Center Livestock

Email: itjerena7@gmail.com

doctor of biological sciences

Russian Federation, Sankt-Peterburg-Pushkin, Moscovskoe sh., 55 a

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2. Fig. 1. The effect of various compounds on the functional status of intact bull sperm. Horizontal: 1 - cells without incubation; 2 - cells incubated for 4 hours without additives (K); 3 - K + caffeine at a concentration of 2 mm; 4 - K + PRL and GTP in a concentration of 10 ng / ml and 10 μM, respectively. The difference is significant at h: e; h: b; i: c; i: l; g: a; g: j - P <0.001, h: k - P <0.05.

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3. Fig. 2. The effect of pre-incubation of bull sperm before freezing on the functional status of cells after thawing. Horizontal: 1 - cells without incubation; 2 - cells incubated for 4 hours without additives (K); 3 - K + caffeine at a concentration of 2 mm; 4 - K + PRL and GTP in a concentration of 10 ng / ml and 10 μM, respectively. The difference is significant at h: k - P <0.001, i: l - P <0.01, h: e; i: f - P <0.05.

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