Panicle formation order influence in rice Oryza sativa L in vitro androgenesis

Cover Page

Cite item

Full Text

Abstract

A comparative analysis of rice Oryza sativa L. main and lateral panicles in androgenesis in vitro on F1 plants of two hybrid combinations: L×3P and K×3P to increase the number of cultivated anthers during the season was carried out. Donor plants were grown on the growing area in metal vessels filled with soil. The main panicle was used from three L×3P hybrids and one K×3P hybrid, single lateral panicle was cut from six L×3P plants and three K×3P plants. The genotypes number was calculated as the product of the calli number with doubled haploids by 1.25. In vitro culture 2050 anthers were introduced. According to the intensity of callus formation, the number of green regenerants per callus, and the doubled haploids proportion, there were no statistically significant differences between the main and lateral panicles in the L×3P combination. In total, 215 doubled haploids lines of twenty nine genotypes were obtained, which is theoretically sufficient for breeding a new variety. On the hybrid combination K×3P, 120 doubled haploids of five genotypes were formed from one donor plant with the main panicle. There were no statistically significant differences between the main and lateral panicles. It is concluded that it is expedient to use lateral rice panicles in vitro androgenesis to create the initial breeding material. Their use makes it possible to distribute labor force over time and increase the total volume of anthers cultivated in vitro, and, ultimately, the doubled haploids number and variability of one sample. In the time absence and other restrictions, it is preferable to use the main panicle.

About the authors

M. V Ilyushko

Federal Scientific Centre of Agrobiotechnology of the Far East named A.K. Chaika, 692539

Email: ilyushkoiris@mail.ru
Primorskiy kray, pos. Timiryasevskiy, ul. Volozhenina, 30

M. V Romashova

Federal Scientific Centre of Agrobiotechnology of the Far East named A.K. Chaika, 692539

Primorskiy kray, pos. Timiryasevskiy, ul. Volozhenina, 30

S. S Guchenko

Federal Scientific Centre of Agrobiotechnology of the Far East named A.K. Chaika, 692539

Primorskiy kray, pos. Timiryasevskiy, ul. Volozhenina, 30

References

  1. Сельдимирова О.А., Титова Г.Е., Круглова Н.Н. Комплексный морфолого-гистологический подход к изучению морфогенных структур в культуре in vitro пыльников пшеницы // Известия РАН. Серия биологическая. 2016. № 2. С. 155-161. doi: 10.7868/S0002332916020089.
  2. Study of androgenic plant families of alloplasmic introgression lines (H. vulgare) - T. aestivum and the use of sister DH lines in breeding / L. Pershina, N. Trubacheeva, E. Badaeva, et al. // Plants. 2020. Vol. 9. URL: https://pdfs.semanticscholar.org/0f17/6f055840695843ec653e6e8584cfaf14eb2f.pdf? (дата обращения: 16.03.2022). doi: 10.3390/plants9060764.
  3. Tripathy S.K. High-throughput doubled haploid production for indica rice breeding / ed. J.M. Segui-Simarro // Doubled haploid technology. Methods molecular biology. New Yoek: Humana, 2021. Vol. 2287. P. 343-363. doi: 10.1007/978-1-0716-315-3_20.
  4. Goncharova Y.K.,Vereshchagina S.A., Gontcharov S.V. Nutrient media for double haploid production in anther culture of rice hybrids // Plant Cell Biotechnology and Molecular Biology. 2020. Vol. 20. No. 23-24, P. 1215-1223.
  5. Hooghvorst I., Ferreres I., Nogues S. Anther culture and chromosome doubling in Mediterranean japonica rice / ed. J.M. Segui-Simarro // Doubled haploid technology. Methods in molecular biology. New Yoek: Humana, 2021. Vol. 2287. P. 333-341. doi: 10.1007/978-1-0716-315-3_19.
  6. Haploids: constraints and opportunities in plant breeding / S.L. Dwivedi, A.B. Brite, L. Tripathi, et al. // Biotechnology Advances. 2015. Vol. 33. N 6. P. 812-829. doi: 10.1016/j.biotechadv.2015.07.001.
  7. High-frequency androgenic green plant regeneration in indica rice for accelerated breeding / S. Samantaray, B. Dash, S.S. Bhuyan, et al. / eds. S.S. Gosal, S.H.Wani. Accelerated plant Breeding. 2020. Vol. 1. Springer, Cham. URL: https://link.springer.com/content/pdf/10.1007/978-3-030-41866-3.pdf (дата обращения: 16.03.2022). doi: 10.1007/978-3-030-41866-3_10.
  8. Dunwell J.M. Haploids in flowering plants: origins and exploitation // Plant Biotechnology Journal. 2010. Vol. 8. P. 377-424. doi: 10.1111/j.1467-7652.2009.00498.x
  9. Niazian M., Shariatpanahi M.E. In vitro-based doubled haploid production: recent improvements // Euphitica. 2020. Vol. 216. Article 69. URL: https://link.springer.com/article/10.1007/s10681-020-02609-7 (дата обращения: 01.02.2022). doi: 10.1007/s10681-020-02609-7.
  10. Murovec J., Bohanec B. Haploids and doubled haploids in plant breeding / ed. I. Abdurakhmonov. Plant breeding. 2012. P. 87-106. URL: https://www.intechopen.com/chapters/25554 (дата обращения 16.03.2022). doi: 10.5772/29982.
  11. Илюшко М.В., Скапцов М.В., Ромашова М.В. Содержание ядерной ДНК у регенерантов риса (Oryza sativa L.), полученных в культуре пыльников in vitro // Сельскохозяйственная биология. 2018. Т. 53. № 3. С. 531-538. doi: 10.15389/agrobiology.2018.3.531rus.
  12. Ван дер Варден Б.Л. Математическая статистика / пер. с нем. М.: Иностранная литература, 1960. 450 с.
  13. Лакин Г.Ф. Биометрия. М.: Высш. Школа, 1980. 293 с.
  14. Ilyushko M.V., Romashova M.V., Guchenko S.S.Intra-callus genetic variability of rice Oryza sativa L. doubled haploids regenerated throuth androgenesis in vitro / eds. A. Muratov, S. Ignateva. Fundamental and applied scientific research in the development of agriculture in the Far East (AFE-2021). Lecture Notes in Networks and Systems. Cham.: Springer, 2022. Vol. 353. P. 9-18. doi: 10.1007/978-3-030-91402-8_2.
  15. Илюшко М.В. Регенерационный максимум в андрогенных каллусных линиях риса Oryza sativa L. in vitro // Рисоводство. 2019. № 2(43). С. 29-32.
  16. Сельдимирова О.А., Круглова Н.Н. Андроклинный эмбриоидогенез in vitro злаков // Успехи современной биологии. 2014. Т. 134. № 5. С. 476-487.
  17. Antimitotic and hormone effects on green double haploid plant production through anther culture of Mediterranean japonica rice / I. Hooghvorst, E. Ramos-Fuentes, C. Lopez-Cristofannini, et al. // Plant Cell Tissue Organ Culture. 2018. Vol. 134. P. 205-215. doi: 10.1007/s11240-018-1413-x.
  18. Callus induction and regeneration from anther cultures of Indonesian indica black rice cultivar / A. Maharani, W.I.D. Fanata, F.N. Laeli, et al. //j. Crop Sci. Biotech. 2020. Vol. 23. No. 1. P. 21-28. doi: 10.1007/s12892-019-0322-0.
  19. Exploring factors affecting anther culture in rice (Oryza sativa L.) / S.K. Tripathy, D. Swain, P.M. Mohapatra, et al. // Journal of Applied Biology and Biotechnology. 2019. Vol. 7(02). P. 87-92. doi: 10.7324/JABB.2019.70216.
  20. Sarao N.K., Gosal S.S. In vitro androgenesis for accelerated breeding in rice / eds. S.S. Gosal, S.H. Wani // Biotechnologies of crop improvement. Cham.: Springer, 2018. Vol. 1. P. 407-435. doi: 10.1007/978-3-319-78283-6_12.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2023 Russian Academy of Sciences