Mode of action of bacteria bacillus subtilis № 2 and humic preparation on fruit formation in sweet pepper

Cover Page

Cite item

Full Text

Open Access Open Access
Restricted Access Access granted
Restricted Access Subscription or Fee Access

Abstract

The effectivity of action of bacteria Bacillus subtilis № 2 and humic preparation Stimulife on yield and fruit formation in sweet pepper (Capsicum annuum L.) in technologies of separated and joint use was studied. It was established that the use of bacteria Bacillus subtilis № 2 led to a decrease in the coefficient of variation in the mass of the leaves of the pepper plants and the coefficient of variation in the masses of the fruits of the plants. The maximum directivity of action of the preparations studied was revealed with the help of graph analysis. The action of bacteria Bacillus subtilis № 2 is directed on increasing the number of leaves of pepper plants, HP Stimulife - on reducing the vegetative period of plants, and joint application – on increasing the number of fruits on a single plant. In factor analysis, it was shown that a negative synergistic effect appeared with respect to chlorophyll (а+b) and the duration of the growth stages when Bacillus subtilis № 2 and HP Stimulife were combined used. The variety differences in the reactions of pepper plants to the preparations used were revealed.

Full Text

Restricted Access

About the authors

V. N. Pishchik

Agrophysical Research Institute; Scientific Institution All-Russian Research Institute for Agricultural Microbiology

Author for correspondence.
Email: veronika-bio@rambler.ru

candidate of biological sciences

Russian Federation, 196600, Sankt-Peterburg, Grazhdanskiy pr., 14; Sankt.-Petersburg, Pushkin, 196608, sh. Podbelsky, 3

N. I. Vorobyov

Scientific Institution All-Russian Research Institute for Agricultural Microbiology

Email: veronika-bio@rambler.ru

candidate of technical sciences

Russian Federation, Sankt.-Petersburg, Pushkin, 196608, sh. Podbelsky, 3

O. R. Udalova

Agrophysical Research Institute

Email: veronika-bio@rambler.ru

candidate of agricultureal sciences

Russian Federation, 196600, Sankt-Peterburg, Grazhdanskiy pr., 14

V. G. Surin

Center for Interdisciplinary Research on Environmental Problems of the Russian Academy of Sciences

Email: veronika-bio@rambler.ru

candidate of physics-mathematical sciences

Russian Federation, Sankt. Petersburg, nab. Kutuzovat, 14

Yu. V. Khomyakov

Agrophysical Research Institute

Email: veronika-bio@rambler.ru

candidate of biological sciences

Russian Federation, 196600, Sankt-Peterburg, Grazhdanskiy pr., 14

A. A. Popov

Center for Interdisciplinary Research on Environmental Problems of the Russian Academy of Sciences

Email: veronika-bio@rambler.ru

candidate of agricultural sciences

Russian Federation, Sankt. Petersburg, nab. Kutuzovat, 14

References

  1. Завалин А.А. Биопрепараты, удобрения и урожай. − М.: ВНИИА, 2005. − 302 с.
  2. Ahemad M., Kibret M. Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. // J. King Saud Uni. Sci. − 2014. − V. 26. – P. 1-20.
  3. Чеботарь В.К., Петров В.Б., Шапошников А.И., Кравченко Л.В. Биохимические критерии оценки агрономически значимых свойств бацилл, используемых при создании микробиологических препаратов. // Сельскохозяйственная биология. – 2011. − № 3. − С. 119-122.
  4. Кожемяков А.П., Белоброва С.Н., Орлова А.Г. Создание и анализ базы данных по эффективности микробных препаратов комплексного действия. // Сельскохозяйственная биология. − 2011. − № 3. − С. 112-115.
  5. Ruzzi M., Aroca R. Plant growth-promoting rhizobacteria act as biostimulants in horticulture. // Sciencia Horticulturae − V.196. – P. 124-134.
  6. http://www.agrophys.com/Agrophys_files/Stimullife/stimullife.html.
  7. Панова Г.Г., Черноусов И.Н., Удалова О.Р., Александров А.В., Карманов И.В., Аникина Л.М., Судаков В.Л. Научно-технические основы круглогодичного получения высоких урожаев качественной растительной продукции при искусственном освещении. // Доклады Россельхозакадемии. – 2015. − №4. − С. 17-21.
  8. Ермаков Е.И., Желтов Ю.И., Мильто Н.Е., Кучеров В.И. Почвогрунт для выращивания растений «Агрофит» // Патент №2081555 РФ. − БИ №17. − 1997.
  9. Lichtenthaler H.G.. Chlorophylls and carotenoids pigments of photosynthetic biomembranes // Methods Enzymol. – 1987. – V.148. –350-382.
  10. Ермаков А.И., Арасимович В.В., Ярош Н.П., Перуанский Ю.В., Луковникова Г.А., Иконникова М.И. Методы биохимического анализа растений. – Л.: Агропромиздат, 3-е изд., 1987. – 430 C.
  11. Pishchik V.N., Vorobyov N.I., Walsh O.S., Surin V.G., Khomyakov Y.V. Estimation of synergistic effect of humic fertilizer and Bacillus subtilis on lettuce plants by reflectance measurements // Journal of Plant Nutrition. – 2016. – V.39. – №8. –. Р. 1074-1086.
  12. Воробьев Н.И., Свиридова О.В., Кутузова Р.С. Методические рекомендации по использованию граф-анализа в исследованиях систем, состоящих из биотических и абиотических компонентов. – СПб.: ВНИИСХМ, 2006. – 58 C.
  13. Воробьев Н.И., Проворов Н.А., Пищик В.Н., Свиридова О.В. Программа вычисления уровня синергизма – супрессии в ответной реакции биологических объектов на двухфакторное воздействие // Свидетельство о государственной регистрации программы для ЭВМ № 2016618107. – Регистрация в Реестре программ для ЭВМ 21.07, 2016.
  14. Garcia J.A.I., Probanza A., Ramos B., Palomino M.R., Manero F.J.G. Effect of inoculation of Bacillus lichenoformis on tomato and pepper // Agronomie. – 2004. – V.24. – №4. – P.169-176.
  15. Varanini Z, Pinton R. Direct versus indirect effects of soil humic substances on plant growth and nutrition / Pinton R, Varanini Z, Nannipieri P, eds. // In: The Rizosphere. Basel: Marcel Dekker. – 2001. – P. 141-58.
  16. Прохоренко Н.Б., Пахомова В.М., Хабиров Р.Н., Даньшина Е.В. Морфологические параметры и урожайность у растений яровой пшеницы сорта Люба при оптимизации минерального питания // Сельскохозяйственная биология. – 2008. – № 5. – C. 43-47.
  17. Pishchik V.N., Vorobyev N.I. , Ostankova Yu.V., Semenov A.V., Totolian Areg A., Popov A.A., Khomyakov Y.V. , Udalova O.R., Shibanov D.V., Vertebny V.E., Dubovitskaya V.I., Sviridova O.V., Walsh O.S., Shafian S. Impact of Bacillus subtilis on tomato plants growth and some biochemical characteristics under combined application with humic fertilizer. // International Journal of Plant & Soil Science. – 2018. – V.22. – P.1-12.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download
2. Fig. 1. The mass distribution density of leaves and fruits of the Ermak plant variety in the variants: K - control, C - Stimulyf, B - Bacillus subtilis No. 2, C + B - Stimulus + Bacillus subtilis No. 2.

Download (578KB)
Indexing metadata
3. Fig. 2. Density of mass distribution of leaves and fruits of the Cinderella variety plant in variants: C - control, C - Stimulife, B - Bacillus subtilis No. 2, C + B - Stimulus + Bacillus subtilis No. 2.

Download (560KB)
Indexing metadata
4. Fig. 3. The graph of direct connections between the measured characteristics of plants and the action of drugs. H - Cinderella variety; E - grade Ermak; 1 - the number of fruits, pieces / plant; 2 - the mass of fruits, g / plant; 3 - leaf mass, g / plant; 4 - the number of leaves, pieces / plant; 5 - the amount of chlorophylls, mg / g of leaves; 6 - height of plants, cm; 7 - the length of the growing season, days; 8 - Stimulyf; 9 - Bacillus subtilis No. 2; 10 - Stimulyf + Bacillus subtilis No. 2.

Download (142KB)
Indexing metadata

Copyright (c) 2019 Russian academy of sciences