Morphological characteristics of the oocyte–cumulus complexes bos taurus and bos indicus of different purpose productivity

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In the technology of in vitro embryo production in bovines, qualitative and quantitative indicators of the initial population of viable oocytes determine the successful output of blastocysts suitable for native embryotransfer or vitrification Therefore the study of reproductive potential of cows Bos taurus and Bos indicus (zebu cattle) of different directions of productivity by the method of assessing the quality and quantity of oocytes is of scientific and practical interest. The aim of this study was to conduct a comparative analysis of Russian and Brazilian cattle on the yield of oocyte–cumulus complexes (OCC) and their morphological characteristics in the populations of dairy and beef cattle productivity. The following samples of cows were formed:  Aberdeen–Angus breed n=82 (Russian Federation);  black–and–white breed n=62 (Russian Federation); Nelore n=149 (Brazil);  Gir n=63 (Brazil). The material of the study was oocyte–сumulus complexes obtained in the result of transvaginal puncture of follicles (transvaginal aspiration of oocytes TAO, ovum pick–up OPU). The quality of OCC was assessed by the following morphological parameters: the presence of cumulus layer and its quality, oocytes size, homogeneity and color of ooplasm, turgor. The number of viable OCC from the total amount is: Aberdeen Angus 67.15%; black–mottled 42%; Nelore 83.9%; Gir 71,4%.  Thus, Bos indicus (zebu cattle) is more promising for the technology of production embryos in vitro, as it exceeds Bos taurus by more than 2 times in the output of oocytes, both in the group of beef cattle and in the group of dairy.

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Sobre autores

L. Rotar

Saint–Petersburg State Agrarian University

Autor responsável pela correspondência
Email: valevskaya@bk.ru

postgraduate student

Rússia, 19660, 1 Sankt–Peterburg, Pushkin, Peterburgskoe sh., 2

J. Souza

Labor Brio embryo Assessoria Agropecuaria e Biotecnologia Ltda, Araguaina–TO, rua Ademar Vicente Ferreira

Email: valevskaya@bk.ru
Rússia, n. 2241, setor Senador, Brazil

Bibliografia

  1. Кыса И.С. Ускоренное воспроизводство высокопродуктивных племенных животных в молочном и мясном скотоводстве на основе новых биотехнологических методов: Автореферат диссертации на соискание ученой степени доктора биологических наук.– Дубровицы, 2000.
  2. Gordon I. Laboratory production of cattle embryos: Монография. – Dublin, 2003. – 548 p.
  3. Пестис В.К., Голубец Л.В., Дешко А.С., Старовойтова М.П., Стецкевич Е.К., Кысса И.С., Якубец Ю.А., Попов М.В. Эффективность получения ооцитов методом трансвагинальной аспирации у коров–доноров// Сельское хозяйство – проблемы и перспективы Сб. науч. тр. под ред. чл.–корр. НАН Беларуси В. К. Пестиса.– Т. 26 ЗООТЕХНИЯ. – Гродно: ГГАУ, 2014.– 123с.
  4. Карымсаков Т.Н., Бекенов Д.М., Спанов А.А. Сравнительные результаты приживляемости сексированных эмбрионов, полученные методами in vivo и in vitro // Молочное и мясное скотоводство.– 2017. – № 6 – С. 9–11
  5. Souza J.F., .Oliveira C.M., Lienou L.L., Cavalcante T.V., Alexandrino E., Santos R.R., Rodrigues A.P.R., Campello C.C., Figueiredo J.R., Dias F.E.F. Vitrification of bovine embryos followed by in vitro hatching and expansion// Zygote.– 2018. – № 1 (26).– P. 99–103.
  6. Ротарь Л.Н., Souza J.F. Морфологическая оценка ооцит–кумулюсных комплексов коров разного типа продуктивности в технологии получения эмбрионов in vitro// Качественный рост российского агропромышленного комплекса:возможности, проблемы и перспективы: Материалы деловой программы XXVII международной агропромышленной выставки «АГРОРУСЬ – 2018» – СПб: СПбГАУ, 2018. – 318 с.
  7. Ларкин Д.М., Юдин Н.С. Значимость геномных исследований для понимания истории формирования домашних животных // Молекулярная генетика, микробиология и вирусология.– 2016.– № 4,– С. 123–128.
  8. Столповский Ю. А., Ахани Азари М., Евсюков А.Н., Кол Н.В., Рузина М.Н., Воронкова В.Н., Cулимова Г.Е. Сравнительный анализ полиморфизма ISSR маркеров у пород крупного рогатого скота // Генетика.–2011.– № 2 (47). – С. 213–226.
  9. Bastos M.R., Mattos M.C.C., Meschiatti M.A.P., Surjus R.S., Guardieiro M.M., Ferreira J.C.P. Ovarian function and circulating hormones innonlactating Nelore versus Holstein cow s// Acta Scientiae Veterinariae.– 2010.– № 38 (2).–776 р.
  10. Nagano M., Katagiri S., Takahashi Y. Relationship between bovine oocyte morphology and in vitro developmental // Zygote.– 2006.– № 14.– P.53–61.
  11. Baldrighi J.M., Sa Filho M.F., Batista E.O.S., Lopes R.N.V.R. Visintin J.A., Baruselli P.S. Anti–Mullerian hormone concentration and antral ovarian follicle population in Murrah heifers compared to Holstein and Gyr kept under the same management// Reproduction In Domestic Animals.– 2014.– № 49.– P. 1015–1020.
  12. Alvarez P., Spicer L.J., Chase C.C., Payton M.E., Hamilton T.D., Stewart R.E. Ovarian and endocrine characteristics during an estrous cycle in Angus, Brahman, and Senepol cows in a subtropical environment// Journal of Animal Science.– 2000.– № 78.–P. 1291–1302.
  13. Sartori R., Gimenes L.U.,. Monteiro P.L.J., Melo L.F., Baruselli P.S., Bastos M.R. Metabolic and endocrine differences between Bos Taurus and Bos indicus females that impact the interaction of nutrition with reproduction// Theriogenology.–2016.– № 86.– P. 32–40.
  14. Baruselli P.S., Batista E.O.S., Vieira L.M.,. Ferreira R.M, Guerreiro B.G., Bayeux B.M., Sales J.N.S., Souza A.H., Gimenes L.U. Factors that interfere with oocyte quality for in vitro production of cattle embryos: effects of different developmental & reproductive stages Animal Reproduction.–2016.–№ 3 (13).– P.264–272.
  15. Sartori R., Guardieiro M.M., Surjus R.S., Melo L.F., Prata A.B., Ishiguro M. Metabolic hormones and reproductive function in cattle// Animal Reproduction.– 2013.– № 10.– P.199–205.
  16. Sales J.N.S., Iguma L.T., Batista R.I.T.P., Quintao C.C.R., Gama M.A.S., Freitas C. Effects of a high–energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows// Journal of Dairy Science.– 2015.– № 98.– P.3086–99.
  17. Ward F.A., LonerganP., Enright B.P., Boland M.P. Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick–up technology// Theriogenology.– 2000.– № 3 (54).– P. 433–446.
  18. Hyun–Joo Lim, Ho–Beak Yoon, Harim Im, Jihoo Park, Yong–il Cho, Yeon–Seop Jeong, Kwang–Seok Ki, Seok–Ki Im. Survey on the Incidence of Reproductive Disorders in Dairy Cattle // Journal of Embryo Transfer.– 2015.– №1(30).– P.59–64.
  19. Barbat A., Le Mezec P., Ducrocq V., Mattalia S., Fritz S., Boichard D., Ponsart C., Humblot P. Female fertility in French dairy breeds: current situation and strategies for improvement// Journal of Reproduction and Development.–2010.– № 56:– P. 15–21
  20. Meza F.J.T., Souza J.F., Rosales J.H., Angel H.R., Rodríguez A.P., Lucero F.A.M., González A.R. De la congelación de semen y la inseminación artificial a la aspiración folicular y fecundación in vitro, 1a Reunion Regional sobre Reproduccion Asistida en bovines// La biotecnología de la reproducción en rumiantes y su aplicación en la ganadería Mexicana, Torreon Coah, Mexico.– 2018.
  21. Pontes J.H.F., Silva K.C.F., Basso A.C., Rigo A.G., Ferreira C.R. , Santos G.M.G., Sanches B.V., Porcionato J.P.F., Vieira P.H.S., Faifer F.S., Sterza F.A.M, Schenk J.L., Seneda M.M. Large–scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus–taurus dairy cows using sexed sperm // Theriogenology.– 2010.– № 74 P. 1349–1355

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