Informative value of blood l-lactate and integral leukocyte indices for predicting severe course of mycoplasmosis in calves

Capa

Citar

Texto integral

Resumo

The aim was to study the informativity of blood L-lactate and integral leukocyte indices for predicting a severe course of mycoplasmosis in calves during their natural infection with M. bovis. Daily clinical observation of 36 Holstein calves naturally infected with M. bovis (at the age of 10-15 days) was carried out for one month on the farms of the Ural Federal District. In blood samples obtained from the jugular vein of animals at the first signs of the disease, the levels of hemoglobin, erythrocytes, leukocytes, platelets, hematocrit on Abacus Junior Vet analyzer (Diatron, Austria), L-lactate concentration - by reaction with paraoxydiphenyl on UV-1800 spectrophotometer (Shimadzu, Japan) were studied. Differential leukocyte counts in Romanowsky- Giemsa stained blood smears were performed using Olympus BX43 microscope (Olympus, Japan). The following integral leukocyte indices were calculated: Krebs index (KI), leukocyte index of intoxication by Y. J. Calf- Calif (LII), neutrophil reactive response by T. Sh. Khabirov (NRR), adaptation index (AI), Bredeck index (IB), nuclear shift index (NSI), lymphocyte- granulocyte index (ILG), neutrophil to monocyte ratio index (NMR), leukocyte shift index (LSI), lymphocyte to monocyte ratio index (LMR) and lymphocyte to neutrophil ratio (LNR). In 22 (61,1 %) calves a mild to moderately severe course was detected, in 14 (38,9 %) - a severe course of mycoplasmosis. The animals predisposed to a severe course of mycoplasmosis had higher blood L-lactate > 1.68 mmol/l, AI > 1.57 units, ILG > 16.7 units, LNR > 1.41 units, reduced KI < 0.645 units and LSI < 0.600 units at the first clinical signs of disease. The information value of predictors for prognosing severe mycoplasmosis in calves was evaluated as very good; the sensitivity was 71.4-85.7 %, the specificity made 81.8-90.9 %. The accumulation of L-lactate in the blood of animals and changes in leukocyte indices (AI, ILG, LNR, KI, LSI) were shown to be interrelated.

Sobre autores

A. Chernitskiy

Ural Federal Agrarian Scientific Research Center,Ural Branch of the Russian Academy of Sciences

Email: cherae@mail.ru
620142, Ekaterinburg, ul. Belinskogo, 112a

I. Shkuratova

Ural Federal Agrarian Scientific Research Center,Ural Branch of the Russian Academy of Sciences

620142, Ekaterinburg, ul. Belinskogo, 112a

A. Poryvaeva

Ural Federal Agrarian Scientific Research Center,Ural Branch of the Russian Academy of Sciences

620142, Ekaterinburg, ul. Belinskogo, 112a

E. Pechura

Ural Federal Agrarian Scientific Research Center,Ural Branch of the Russian Academy of Sciences

620142, Ekaterinburg, ul. Belinskogo, 112a

O. Tomskikh

Ural Federal Agrarian Scientific Research Center,Ural Branch of the Russian Academy of Sciences

620142, Ekaterinburg, ul. Belinskogo, 112a

Bibliografia

  1. Mycoplasma bovis infections in cattle / F. P. Maunsell, A. R. Woolums, D. Francoz, et al. //j. Vet.Intern. Med. 2011. Vol. 25. No. 4. P. 772-783. doi: 10.1111/j.1939-1676.2011.0750.x.
  2. Mycoplasma bovis infections - occurrence, diagnosis and control / K. Dudek, R.A.J. Nicholas, E. Szacawa, et al. // Pathogens. 2020. Vol. 9. No. 8. 640. URL: https://www.mdpi.com/2076-0817/9/12/994 (дата обращения: 16.02.2023). doi: 10.3390/pathogens9080640.
  3. A review of Mycoplasma diagnostics in cattle / A. M. Parker, P. A. Sheehy, M. S. Hazelton, et al. //j. Vet.Intern. Med. 2018. Vol. 32. No. 3. P. 1241-1252. doi: 10.1111/jvim.15135.
  4. Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis / E. V. Remizova, A. V. Gorbatov, L. K. Semina, et al. // Veterinary Science Today. 2022. Vol. 11. No. 4. P. 335-340. doi: 10.29326/2304-196X-2022-11-4-335-340.
  5. Perez-Casal J. Pathogenesis and virulence of Mycoplasma bovis // Vet. Clin. North Am. Food Anim. Pract. 2020. Vol. 36. No. 2. P. 269-278. doi: 10.1016/j.cvfa.2020.02.002.
  6. The upper respiratory tract microbiome and its potential role in bovine respiratory disease and otitis media / S. F. Lima, A.G.V. Teixeira, C. H. Higgins, et al. // Sci. Rep. 2016. Vol. 6. 29050. URL: https://www.nature.com/articles/srep29050 (дата обращения: 16.02.2023). doi: 10.1038/srep29050.
  7. Mycoplasma bovis arthritis and pneumonia in calves in Jordan: An emerging disease / W. M. Hananeh, W.M.A. Momani, M. M. Ababneh, et al. // Vet. World. 2018. Vol. 11. No. 12. P. 1663-1668. doi: 10.14202/vetworld.2018.1663-1668.
  8. Методическое пособие по прогнозированию и ранней диагностике респираторных болезней у телят / А. Е. Черницкий, Л. И. Ефанова, А. И. Золотарев и др. Воронеж: Истоки, 2013. 48 с. doi: 10.13140/RG.2.2.11326.28481.
  9. Clinical, behavioral, and pulmonary changes in calves following inoculation with Mycoplasma bovis / B. J. White, D. E. Anderson, D. G. Renter, et al. // Am. J. Vet. Res. 2012. Vol. 73. No. 4. P. 490-497. doi: 10.2460/ajvr.73.4.490.
  10. Assessment of L-lactatemia as a predictor of respiratory disease recognition and severity in feedlot steers / S. Buczinski, R. D. Rademacher, H. M. Tripp, et al. // Prev. Vet. Med. 2015. Vol. 118. No. 4. P. 306-318. doi: 10.1016/j.prevetmed.2014.12.003.
  11. Clinical utilization of point-of-care blood L-lactate concentrations in naturally occurring respiratory disease in feedlot cattle / M. Zeineldin, M. Ghanem, Y. A. El-Raof, et al. // Pak. Vet. J. 2017. Vol. 37. No. 2. P. 210-214.
  12. Lactate regulates metabolic and pro-inflammatory circuits in control of T cell migration and effector functions / R. Haas, J. Smith, V. Rocher-Ros, et al. // PLoS Biol. 2015. Vol. 13. No. 7. e1002202. URL: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.1002202 (дата обращения: 16.02.2023). doi: 10.1371/journal.pbio.1002202.
  13. Lactate at the crossroads of metabolism, inflammation, and autoimmunity / V. Pucino, M. Bombardieri, C. Pitzalis, et al. // Eur. J. Immunol. 2017. Vol. 47. No. 1. P. 14-21. doi: 10.1002/eji.201646477.
  14. Lactate released by inflammatory bone marrow neutrophils induces their mobilization via endothelial GPR81 signaling / E. Khatib-Massalha, S. Bhattacharya, H. Massalha, et al. // Nat.Commun. 2020. Vol. 11. No. 1. 3547. URL: https://www.nature.com/articles/s41467-020-17402-2 (дата обращения: 16.02.2023). doi: 10.1038/s41467-020-17402-2.
  15. Individual reactivity of granulocytic system of newborn calves and its role in pathogenesis of inflammatory diseases of respiratory and gastrointestinal tracts / Sidel'nikova V.I., Chernitskiy A. E., Zolotarev A. I., et al. // Agricultural biology. 2015. Vol. 50. No. 4. P. 486-494. doi: 10.15389/agrobiology.2015.4.486eng.
  16. Чуличикова С.А., Дерхо М. А. Лейкоцитарные индексы как индикатор иммунного статуса организма коров на ранних сроках стельности // АПК России. 2016. Т. 75. № 1. С. 47-51.
  17. Возрастные изменения интегральных гематологических индексов у крупного рогатого скота / А. П. Жуков, Е. Б. Шарафутдинова, А. П. Датский и др. // Известия Оренбургского государственного аграрного университета. 2016. № 4 (60). С. 213-216.
  18. McGuirk S.M., Peek S. F. Timely diagnosis of dairy calf respiratory disease using a standardized scoring system // Anim. Health Res. Rev. 2014. Vol. 15. No. 2. P. 145-147. doi: 10.1017/S1466252314000267.
  19. Pino R.M., Singh J. Appropriate clinical use of lactate measurements // Anesthesiology. 2021. Vol. 134. No. 4. P. 637-644. doi: 10.1097/ALN.0000000000003655.
  20. Сперанский И.И., Самойленко Е. М., Лобачева И. Н. Общий анализ крови - все ли его возможности исчерпаны? Интегральные индексы интоксикации как критерии оценки тяжести течения эндогенной интоксикации, ее осложнений и эффективности проводимого лечения // Здоровье Украины. 2009. № 6 (19). С. 51-57.
  21. DeLong E.R., DeLong D.M., Clarke-Pearson D. L.Comparing the areas under two or more correlated receiver operating characteristic curves: a nonparametric approach // Biometrics. 1988. Vol. 44. No. 3. P. 837-845. doi: 10.2307/2531595.
  22. Analysis of the leukocyte response in calves suffered from Mycoplasma bovis pneumonia / K. Dudek, D. Bednarek, U. Lisiecka, et al. // Pathogens. 2020. Vol. 9, No. 5. 407. URL: https://www.mdpi.com/2076-0817/9/5/407 (дата обращения: 16.02.2023). doi: 10.3390/pathogens9050407.
  23. Effect of Mycoplasma bovis on bovine neutrophils / S. Jimbo, M. Suleman, T. Maina, et al. // Vet. Immunol. Immunopathol. 2017. Vol. 188. P. 27-33. doi: 10.1016/j.vetimm.2017.04.011.
  24. Глотов А.Г., Глотова Т. И. Респираторные болезни телят вирусно-бактериальной этиологии. Новосибирск: Агрос, 2008. 256 с.
  25. Оценка уровней реактивности, интоксикации и активности воспаления у собак при бронхопневмонии с использованием лейкоцитарных индексов / Е. Б. Шарафутдинова, Н. В. Сорокин, А. П. Жуков и др. //Известия Оренбургского государственного аграрного университета. 2022. № 6 (98). С. 204-212. doi: 10.37670/2073-0853-2022-98-6-204-212

Arquivos suplementares

Arquivos suplementares
Ação
1. JATS XML
Baixar

Declaração de direitos autorais © Russian Academy of Sciences, 2023