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Immunogenicity of mRNA encoding RBD SARS-CoV-2 in complex with a polycationic carrier
- Authors: Rudometov A.P.1, Sharabrin S.V.1, Borgoyakova M.B.1, Volosnikova E.A.1, Rudometova N.B.1, Orlova L.A.1, Ilyichev A.A.1, Karpenko L.I.1
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Affiliations:
- State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
- Issue: Vol 22, No 2 (2022)
- Pages: 243-248
- Section: Conference proceedings
- URL: https://journals.eco-vector.com/MAJ/article/view/108601
- DOI: https://doi.org/10.17816/MAJ108601
- ID: 108601
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Abstract
BACKGROUND: RBD, receptor-binding domain, a key region of the SARS-CoV-2 surface glycoprotein for virus binding to host cell receptors and one of the targets of virus-neutralizing antibodies. That is why RBD is a promising immunogen for the development of vaccines that can provide protection against COVID-19. Vaccine mRNA is one of the new and rapidly developing vaccine platforms, and the delivery system is a very important component of it.
AIM: The aim of this work was to present the results of a study of the antigenic properties of mRNA encoding the receptor-binding domain of SARS-CoV-2 when administered in combination with a polycationic carrier.
MATERIALS AND METHODS: Dynamic and electrophoretic light scattering were used to characterize mRNA complexes with a polyglucin-spermidine conjugate. To assess the immunogenicity of mRNA were immunized BALB/c mice. The specific activity of the sera was assessed using enzyme immunoassay.
RESULTS: As a result, the sizes and surface charge of the RBD-encoding mRNA complexes with the polyglucin-spermidine conjugate were determined. It has been shown that wrapping mRNA in a polyglucin-spermidine conjugate shell leads to an increase in the induction of RBD-specific antibodies in BALB/c mice compared to naked mRNA.
CONCLUSIONS: An mRNA encoding the receptor-binding domain of SARS-CoV-2 has been obtained. It has been shown that the packaging of mRNA into the polyglucin-spermidine conjugate shell leads to an increase in immunogenic properties.
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About the authors
Andrey P. Rudometov
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Author for correspondence.
Email: andrei692@mail.ru
ORCID iD: 0000-0003-2808-4309
SPIN-code: 7245-2291
Cand. Sci. (Biol.), Senior Research Associate
Russian Federation, Koltsovo, Novosibirsk RegionSergey V. Sharabrin
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Email: sharabrin_sv@vector.nsc.ru
ORCID iD: 0000-0003-0664-3587
Scopus Author ID: 57221380569
Junior Research Associate
Russian Federation, Koltsovo, Novosibirsk RegionMaria B. Borgoyakova
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Email: borgoyakova_mb@vector.nsc.ru
ORCID iD: 0000-0002-0768-1561
Scopus Author ID: 57221732585
Junior Research Associate
Russian Federation, Koltsovo, Novosibirsk RegionEkaterina A. Volosnikova
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Email: volosnikova_ea@vector.nsc.ru
ORCID iD: 0000-0001-5028-5647
Cand. Sci. (Biol.), Leading Research Associate
Russian Federation, Koltsovo, Novosibirsk RegionNadezhda B. Rudometova
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Email: nadenkaand100@mail.ru
ORCID iD: 0000-0002-1684-9071
SPIN-code: 5283-6608
Cand. Sci. (Biol.), Research Associate
Russian Federation, Koltsovo, Novosibirsk RegionLyubov A. Orlova
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Email: orlova_la@vector.nsc.ru
ORCID iD: 0000-0001-7214-1855
Research Assistant
Russian Federation, Koltsovo, Novosibirsk RegionAlexander A. Ilyichev
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Email: ilyichev@vector.nsc.ru
ORCID iD: 0000-0001-5356-0843
Scopus Author ID: 7006402361
ResearcherId: B-1327-2012
Dr. Sci. (Biol.), Professor, Head of the Department of Bioengineering
Russian Federation, Koltsovo, Novosibirsk RegionLarisa I. Karpenko
State Research Center of Virology and Biotechnology “Vector”, Federal Service for Surveillance on Consumer Rights Protection and Human Well-being
Email: karpenko@vector.nsc.ru
ORCID iD: 0000-0003-4365-8809
SPIN-code: 2026-5992
Scopus Author ID: 7005000410
Dr. Sci. (Biol.), Assistant Professor, Leading Research Associate
Russian Federation, Koltsovo, Novosibirsk RegionReferences
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).