Email this article 
Research of the genetic diversity of human rhinoviruses on the territory of Saint Petersburg 2021–2022
- Authors: Ksenafontov A.D.1, Pisareva M.M.1, Eder V.A.1, Musaeva T.D.1, Kiseleva I.V.2,3
-
Affiliations:
- Smorodintsev Research Institute of Influenza
- Saint Petersburg State University
- Institute of Experimental Medicine
- Issue: Vol 22, No 2 (2022)
- Pages: 89-96
- Section: Original research
- URL: https://journals.eco-vector.com/MAJ/article/view/108734
- DOI: https://doi.org/10.17816/MAJ108734
- ID: 108734
Cite item
Open Access
Access granted
Abstract
BACKGROUND: Respiratory viruses circulate everywhere. Rhinoviruses are the most common cause of human upper respiratory tract infections. Therefore, it is necessary to study circulation of their species and types.
AIM: To study circulation of different species and types of rhinoviruses in Saint Petersburg.
MATERIALS AND METHODS: Detection of rhinoviruses was carried out by real-time PCR using commercial kits AmpliSens ORVI-screen-FL (Central Research Institute of Epidemiology of Rospotrebnadzor, Moscow); the study of the genetic diversity of rhinoviruses was carried out by Sanger sequencing method; alignment of the obtained sequences was carried out in the MAFFT program, the phylogenetic tree was built in the RAxML program, visualization was carried out in FigTree.
RESULTS: The most common species of human rhinoviruses circulating in Saint Petersburg are HRV-A, while the types of rhinoviruses almost never repeat.
CONCLUSIONS: On the territory of St. Petersburg, the genetic diversity of rhinoviruses is very widely represented. From December 2020 to October 2021, 70 rhinoviruses were typed, with HRV-A rhinoviruses being the most common (38 rhinoviruses, or 54%). HRV-C and HRV-B were detected in equal numbers (16 rhinoviruses, or 23% each). Phylogenetic trees of genetic types of rhinovirus species A, B, and C were built. The types of rhinoviruses are highly variable, which complicates their study, as well as the development of vaccines against them. Nevertheless, rhinoviruses significantly affect the epidemiological situation, rhinovirus infections can lead to serious health consequences, so they need to be studied.
Full Text
About the authors
Andrey D. Ksenafontov
Smorodintsev Research Institute of Influenza
Author for correspondence.
Email: ksenandrey@yandex.ru
ORCID iD: 0000-0002-4532-6210
Postgraduate Student, Research Assistant
Russian Federation, Saint PetersburgMaria M. Pisareva
Smorodintsev Research Institute of Influenza
Email: maria.pisareva@influenza.spb.ru
ORCID iD: 0000-0002-1499-9957
SPIN-code: 9662-5361
Scopus Author ID: 6506831021
Cand. Sci. (Biol.), Leading Research Associate
Russian Federation, Saint PetersburgVeronica A. Eder
Smorodintsev Research Institute of Influenza
Email: veronika.eder@influenza.spb.ru
ORCID iD: 0000-0002-9970-3325
SPIN-code: 4793-1377
Dr. Sci. (Biol.), Senior Research Associate
Russian Federation, Saint PetersburgTamila D. Musaeva
Smorodintsev Research Institute of Influenza
Email: tamilamusaeva94@mail.ru
ORCID iD: 0000-0002-3050-1936
SPIN-code: 3767-2899
Scopus Author ID: 57189459858
Junior Research Associate
Russian Federation, Saint PetersburgIrina V. Kiseleva
Saint Petersburg State University; Institute of Experimental Medicine
Email: irina.v.kiseleva@mail.ru
ORCID iD: 0000-0002-3892-9873
SPIN-code: 7857-7306
Scopus Author ID: 7102041346
Dr. Sci. (Biol.), Professor, Head of the Laboratory
Russian Federation, Saint Petersburg; Saint PetersburgReferences
- Chonmaitree T, Alvarez-Fernandez P, Jennings K, et al. Symptomatic and asymptomatic respiratory viral infections in the first year of life: association with acute otitis media development. Clin Infect Dis. 2015;60(1):1–9. doi: 10.1093/cid/ciu714
- Cho GS, Moon BJ, Lee BJ, et al. High rates of detection of respiratory viruses in the nasal washes and mucosae of patients with chronic rhinosinusitis. J Clin Microbiol. 2013;51(3):979–984. doi: 10.1128/JCM.02806-12
- Miller EK, Gebretsadik T, Carroll KN, et al. Viral etiologies of infant bronchiolitis, croup and upper respiratory illness during 4 consecutive years. Pediatr Infect Dis J. 2013;32(9):950–955. doi: 10.1097/INF.0b013e31829b7e43
- Jain S, Self WH, Wunderink RG, et al. CDC EPIC Study Team. Community-acquired pneumonia requiring hospitalization among U.S. adults. N Engl J Med. 2015;373(5):415–427. doi: 10.1056/NEJMoa1500245
- Jain S, Williams DJ, Arnold SR, et al. CDC EPIC Study Team. Community-acquired pneumonia requiring hospitalization among U.S. children. N Engl J Med. 2015;372(9):835–845. doi: 10.1056/NEJMoa1405870
- Greve JM, Davis G, Meyer AM, et al. The major human rhinovirus receptor is ICAM-1. Cell. 1989;56(5):839–847. doi: 10.1016/0092-8674(89)90688-0
- Jensen LM, Walker EJ, Jans DA, Ghildyal R. Proteases of human rhinovirus: role in infection. Methods Mol Biol. 2015;1221:129–141. doi: 10.1007/978-1-4939-1571-2_10
- Tam JC, Bidgood SR, McEwan WA, James LC. Intracellular sensing of complement C3 activates cell autonomous immunity. Science. 2014;345(6201):1256070. doi: 10.1126/science.1256070
- Jacobs SE, Lamson DM, St George K, Walsh TJ. Human rhinoviruses. Clin Microbiol Rev. 2013;26(1):135–162. doi: 10.1128/CMR.00077-12
- Bochkov YA, Watters K, Ashraf S, et al. Cadherin-related family member 3, a childhood asthma susceptibility gene product, mediates rhinovirus C binding and replication. Proc Natl Acad Sci USA. 2015;112(17):5485–5490. doi: 10.1073/pnas.1421178112
- Zlateva KT, van Rijn AL, Simmonds P, et al. Molecular epidemiology and clinical impact of rhinovirus infections in adults during three epidemic seasons in 11 European countries (2007–2010). Thorax. 2020;75(10):882–890. doi: 10.1136/thoraxjnl-2019-214317
- Demirkan E, Kırdar S, Ceylan E, et al. Genotypes of rhinoviruses in children and adults patients with acute respiratory tract infections. Mikrobiyol Bul. 2017;51(4):350–360. (In Turkish). doi: 10.5578/mb.61820
- Panda S, Mohakud NK, Panda S, Kumar S. Epidemiology and phylogenetic analysis of human rhinovirus/Enterovirus in Odisha, Eastern India. Indian J Med Microbiol. 2019;37(4):569–573. doi: 10.4103/ijmm.IJMM_20_23
- Da Costa Souza L, Bello EJM, Dos Santos EM, Nagata T. Molecular and clinical characteristics related to rhinovirus infection in Brasília, Brazil. Braz J Microbiol. 2021;52(1):289–298. doi: 10.1007/s42770-020-00411-0
- Esposito S, Daleno C, Baggi E, et al. Circulation of different rhinovirus groups among children with lower respiratory tract infection in Kiremba, Burundi. Eur J Clin Microbiol Infect Dis. 2012;31(11):3251–3256. doi: 10.1007/s10096-012-1692-9
- Turunen R, Jartti T, Bochkov YA, et al. Rhinovirus species and clinical characteristics in the first wheezing episode in children. J Med Virol. 2016;88(12):2059–2068. doi: 10.1002/jmv.24587
- Zhao Y, Shen J, Wu B, et al. Genotypic diversity and epidemiology of human rhinovirus among children with severe acute respiratory tract infection in Shanghai, 2013–2015. Front Microbiol. 2018;9:1836. doi: 10.3389/fmicb.2018.01836
- Baillie VL, Moore DP, Mathunjwa A, et al. Molecular subtyping of human rhinovirus in children from three Sub-Saharan African countries. J Clin Microbiol. 2019;57(9):e00723–19. doi: 10.1128/JCM.00723-19
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).