The effect methylglyoxal on acute lung injury induced by influenza A(H1N1)PDM09 in mice

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Abstract

The aim of the article. To study of the effect of 2-oxopropanal (methylglyoxal) on virus-induced acute lung injury.

Materials and methods. The study was performed on adult female outbred mice. Methylglyoxal administered subcutaneously at a dose of 50 mg/kg/day to mice for 14 days prior to infection. The pandemic influenza virus A(H1N1)pdm09 was used for modeling viral infection at a dose of 0.75 LD50. Hematology, pathomorphological and histological studies were performed on 4, 7 and 14 days post infection. Level of lung injury was performed by semi-quantitative method.

Results. Methylglyoxal induced 2-fold increase of mortality and lung lesion area (р < 0.05). The structural changes in lung tissue had inflammatory character. These changes had progressive character. The ratio of neutrophiles/lymphocytes was increased by 2.5 times on average in infected animals (р < 0.05 compared to intact animals).

Conclusion. Methylglyoxal aggravated acute lung injury in mice by inducing structural changes in tissue and increased mortality level.

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About the authors

Andrei G. Aleksandrov

Smorodintsev Research Institute of Influenza

Author for correspondence.
Email: forphchemistry@gmail.com
ORCID iD: 0000-0001-9212-3865

a graduate student in the Laboratory of Drug Safety

Russian Federation, Saint Petersburg

Tatiana N. Savateeva-Lyubimova

Smorodintsev Research Institute of Influenza

Email: drugs_safety@mail.ru
ORCID iD: 0000-0003-4516-3308

Professor, MD, PhD in Medicine, Leading Researcher in the Laboratory of Drug Safety

Russian Federation, Saint Petersburg

Arman A. Muzhikyan

Smorodintsev Research Institute of Influenza

Email: vetdiagnostics.spb@gmail.com
ORCID iD: 0000-0002-7093-0014

PhD in Veterinary Sciences, Leading Researcher in the Laboratory of Drug Safety

Russian Federation, Saint Petersburg

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2. Fig. 1. Percent survival during experiment. 1st group — intact mice, 2nd group — infected mice treated with NaCl 0.9%, 3rdgroup — infected mice treated with methylglyoxal. * р > 0,05 compared to intact mice, # р > 0,05 compared to infected mice treated with NaCl 0.9%

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3. Fig. 2. Lung parenchyma of mice. Hematoxylin and eosin staining, magnification ×40 and ×400. a — intact mice; b — 2nd group on 4th day; c — 3rd group on 4th day; d — 2nd group on 7th day; e — 3rd group on 7th day; f — 2nd group on 14th day; g — 3rd group on 14th day

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4. Fig. 2. Lung parenchyma of mice. Hematoxylin and eosin staining, magnification ×40 and ×400. a — intact mice; b — 2nd group on 4th day; c — 3rd group on 4th day; d — 2nd group on 7th day; e — 3rd group on 7th day; f — 2nd group on 14th day; g — 3rd group on 14th day

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Copyright (c) 2019 Aleksandrov A.G., Savateeva-Lyubimova T.N., Muzhikyan A.A.

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